Redox-Activation of Neutrophils Induced by Pericardium Scaffolds
Implantation of scaffolds causes a local inflammatory response whereby the early recruitment of neutrophils is of great importance not only for fighting the infection, but also for facilitating effective regeneration. We used luminol-dependent chemiluminescence, flow cytometry, ELISA, and confocal m...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/24/15468 |
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| author | Irina I. Vlasova Shakir K. Suleimanov Elena V. Mikhalchik Nailya T. Urmantaeva Emin L. Salimov Aligeydar A. Ragimov Tatyana M. Khlebnikova Peter S. Timashev |
| author_facet | Irina I. Vlasova Shakir K. Suleimanov Elena V. Mikhalchik Nailya T. Urmantaeva Emin L. Salimov Aligeydar A. Ragimov Tatyana M. Khlebnikova Peter S. Timashev |
| author_sort | Irina I. Vlasova |
| collection | DOAJ |
| description | Implantation of scaffolds causes a local inflammatory response whereby the early recruitment of neutrophils is of great importance not only for fighting the infection, but also for facilitating effective regeneration. We used luminol-dependent chemiluminescence, flow cytometry, ELISA, and confocal microscopy to assess the responses of neutrophils after the exposure to the scaffold-decellularized bovine pericardium (collagen type I) crosslinked with genipin (DBPG). We demonstrated that DBPG activated neutrophils in whole blood causing respiratory burst, myeloperoxidase (MPO) secretion, and formation of neutrophil extracellular trap-like structures (NETs). In addition, we studied platelets, another important player of the immediate immune host response. We found that platelets triggered redox-activation of isolated neutrophils by the pericardium scaffold, and likely participate in the NETs formation. Free radicals generated by neutrophils and hypochlorous acid produced by MPO are potent oxidizing agents which can oxidatively degrade biological structures. Understanding the mechanisms and consequences of redox activation of neutrophils by pericardium scaffolds is important for the development of new approaches to increase the efficiency of tissue regeneration. |
| first_indexed | 2024-03-09T16:20:46Z |
| format | Article |
| id | doaj.art-7fb300ff73fe44a2be3625635f16ecf1 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:20:46Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7fb300ff73fe44a2be3625635f16ecf12023-11-24T15:21:58ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123241546810.3390/ijms232415468Redox-Activation of Neutrophils Induced by Pericardium ScaffoldsIrina I. Vlasova0Shakir K. Suleimanov1Elena V. Mikhalchik2Nailya T. Urmantaeva3Emin L. Salimov4Aligeydar A. Ragimov5Tatyana M. Khlebnikova6Peter S. Timashev7Institute for Regenerative Medicine, I. M. Sechenov First Moscow State Medical University, Moscow 119991, RussiaInstitute for Regenerative Medicine, I. M. Sechenov First Moscow State Medical University, Moscow 119991, RussiaLopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Moscow 119435, RussiaInstitute for Regenerative Medicine, I. M. Sechenov First Moscow State Medical University, Moscow 119991, RussiaDepartment of Blood Transfusion, I. M. Sechenov First Moscow State Medical University, Moscow 119991, RussiaDepartment of Blood Transfusion, I. M. Sechenov First Moscow State Medical University, Moscow 119991, RussiaInstitute for Regenerative Medicine, I. M. Sechenov First Moscow State Medical University, Moscow 119991, RussiaInstitute for Regenerative Medicine, I. M. Sechenov First Moscow State Medical University, Moscow 119991, RussiaImplantation of scaffolds causes a local inflammatory response whereby the early recruitment of neutrophils is of great importance not only for fighting the infection, but also for facilitating effective regeneration. We used luminol-dependent chemiluminescence, flow cytometry, ELISA, and confocal microscopy to assess the responses of neutrophils after the exposure to the scaffold-decellularized bovine pericardium (collagen type I) crosslinked with genipin (DBPG). We demonstrated that DBPG activated neutrophils in whole blood causing respiratory burst, myeloperoxidase (MPO) secretion, and formation of neutrophil extracellular trap-like structures (NETs). In addition, we studied platelets, another important player of the immediate immune host response. We found that platelets triggered redox-activation of isolated neutrophils by the pericardium scaffold, and likely participate in the NETs formation. Free radicals generated by neutrophils and hypochlorous acid produced by MPO are potent oxidizing agents which can oxidatively degrade biological structures. Understanding the mechanisms and consequences of redox activation of neutrophils by pericardium scaffolds is important for the development of new approaches to increase the efficiency of tissue regeneration.https://www.mdpi.com/1422-0067/23/24/15468neutrophilsplateletspericardium scaffoldsreactive oxygen specieschemiluminescence |
| spellingShingle | Irina I. Vlasova Shakir K. Suleimanov Elena V. Mikhalchik Nailya T. Urmantaeva Emin L. Salimov Aligeydar A. Ragimov Tatyana M. Khlebnikova Peter S. Timashev Redox-Activation of Neutrophils Induced by Pericardium Scaffolds International Journal of Molecular Sciences neutrophils platelets pericardium scaffolds reactive oxygen species chemiluminescence |
| title | Redox-Activation of Neutrophils Induced by Pericardium Scaffolds |
| title_full | Redox-Activation of Neutrophils Induced by Pericardium Scaffolds |
| title_fullStr | Redox-Activation of Neutrophils Induced by Pericardium Scaffolds |
| title_full_unstemmed | Redox-Activation of Neutrophils Induced by Pericardium Scaffolds |
| title_short | Redox-Activation of Neutrophils Induced by Pericardium Scaffolds |
| title_sort | redox activation of neutrophils induced by pericardium scaffolds |
| topic | neutrophils platelets pericardium scaffolds reactive oxygen species chemiluminescence |
| url | https://www.mdpi.com/1422-0067/23/24/15468 |
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