Helicobacter pylori in Gastric biopsy: A Histochemical and Immunohistochemical Assessment
<strong><em>Background:</em></strong> Helicobacter species pylori represent one of the medically prominent and most common infections in the world. Contamination with this microbe has set as a causal factor in the development of gastritis, peptic ulcer, and gastric neoplasia....
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Mosul
2020-01-01
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| Series: | Annals of the College of Medicine Mosul |
| Subjects: | |
| Online Access: | https://mmed.mosuljournals.com/article_164156_027e6ac88ea244ab92643d8d59001f67.pdf |
| Summary: | <strong><em>Background:</em></strong> Helicobacter species pylori represent one of the medically prominent and most common infections in the world. Contamination with this microbe has set as a causal factor in the development of gastritis, peptic ulcer, and gastric neoplasia. Consequently, prompt diagnosis is essential.
<strong><em>Objectives:</em></strong> This study was conveyed to detect <em>H.pylori</em> in gastric biopsies specimens by using routine Hematoxylin, Modified Giemsa dye as well as immunohistochemical stain, besides to assess the specificity and sensitivity of Helicobacter microbe detection in each method.
<strong><em>Patients and methods:</em></strong>The research was both prospective and retrospective, carried out on 100 cases of endoscopically obtained gastric biopsies. Data obtained from archives of the pathology department, at AL-Jamhuri Teaching Hospital, Mosul city, and collected in a period spanning from April 2013 to March 2014. The information included; Age, sex, gastric biopsy location, inflammation status, the presence of dysplasia or carcinoma. <em>Helicobacter pylori</em> infection was assessed histochemically and immunohistochemically.
<strong><em>Results:</em></strong> In a total of 100 gastric samples, patients’ age range was 11 to 82 years (mean age of 46.5 years), with a male to female ratio of 1.38:1. <em>Helicobacter pylori</em> bacilli were positive with H&E/MGS in 71 (71%) of cases, increased to 75 (75%) case with IHC. Chronic gastritis noticed in 85 biopsy specimens, 74% were positive for <em>H.pylori</em>. There was a statistically significant difference between IHC and H&E/MGS (p=0.04) for detection of <em>H.pylori.</em> The sensibility and specificity of the H&E/MGS were measured compared with the recommended standard sensitive and specific IHC test; they were 95% and 100% respectively.
<strong><em>Conclusion:</em></strong> The routine ancillary stains request for the detection of <em>H.pylori</em> remains a laboratory and an institution right. This study revealed that, in our laboratory, the regular application of ancillary dyes is not obliged for the description of <em>H.pylori</em> because it was readily recognizable in the bulk of sections with haematoxylin staining. However, we recommend the use of IHC in specific circumstances. |
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| ISSN: | 0027-1446 2309-6217 |