Helicobacter pylori in Gastric biopsy: A Histochemical and Immunohistochemical Assessment
<strong><em>Background:</em></strong> Helicobacter species pylori represent one of the medically prominent and most common infections in the world. Contamination with this microbe has set as a causal factor in the development of gastritis, peptic ulcer, and gastric neoplasia....
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| Format: | Article |
| Language: | English |
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University of Mosul
2020-01-01
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| Series: | Annals of the College of Medicine Mosul |
| Subjects: | |
| Online Access: | https://mmed.mosuljournals.com/article_164156_027e6ac88ea244ab92643d8d59001f67.pdf |
| _version_ | 1818836039065665536 |
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| author | Zainab Waleed Aziz Shuaib Hashim Saleem Hatim Abdulmajeed Al-Nuaimy |
| author_facet | Zainab Waleed Aziz Shuaib Hashim Saleem Hatim Abdulmajeed Al-Nuaimy |
| author_sort | Zainab Waleed Aziz |
| collection | DOAJ |
| description | <strong><em>Background:</em></strong> Helicobacter species pylori represent one of the medically prominent and most common infections in the world. Contamination with this microbe has set as a causal factor in the development of gastritis, peptic ulcer, and gastric neoplasia. Consequently, prompt diagnosis is essential.
<strong><em>Objectives:</em></strong> This study was conveyed to detect <em>H.pylori</em> in gastric biopsies specimens by using routine Hematoxylin, Modified Giemsa dye as well as immunohistochemical stain, besides to assess the specificity and sensitivity of Helicobacter microbe detection in each method.
<strong><em>Patients and methods:</em></strong>The research was both prospective and retrospective, carried out on 100 cases of endoscopically obtained gastric biopsies. Data obtained from archives of the pathology department, at AL-Jamhuri Teaching Hospital, Mosul city, and collected in a period spanning from April 2013 to March 2014. The information included; Age, sex, gastric biopsy location, inflammation status, the presence of dysplasia or carcinoma. <em>Helicobacter pylori</em> infection was assessed histochemically and immunohistochemically.
<strong><em>Results:</em></strong> In a total of 100 gastric samples, patients’ age range was 11 to 82 years (mean age of 46.5 years), with a male to female ratio of 1.38:1. <em>Helicobacter pylori</em> bacilli were positive with H&E/MGS in 71 (71%) of cases, increased to 75 (75%) case with IHC. Chronic gastritis noticed in 85 biopsy specimens, 74% were positive for <em>H.pylori</em>. There was a statistically significant difference between IHC and H&E/MGS (p=0.04) for detection of <em>H.pylori.</em> The sensibility and specificity of the H&E/MGS were measured compared with the recommended standard sensitive and specific IHC test; they were 95% and 100% respectively.
<strong><em>Conclusion:</em></strong> The routine ancillary stains request for the detection of <em>H.pylori</em> remains a laboratory and an institution right. This study revealed that, in our laboratory, the regular application of ancillary dyes is not obliged for the description of <em>H.pylori</em> because it was readily recognizable in the bulk of sections with haematoxylin staining. However, we recommend the use of IHC in specific circumstances. |
| first_indexed | 2024-12-19T03:00:16Z |
| format | Article |
| id | doaj.art-7fb38ac0124e49f394250e1877ed89da |
| institution | Directory Open Access Journal |
| issn | 0027-1446 2309-6217 |
| language | English |
| last_indexed | 2024-12-19T03:00:16Z |
| publishDate | 2020-01-01 |
| publisher | University of Mosul |
| record_format | Article |
| series | Annals of the College of Medicine Mosul |
| spelling | doaj.art-7fb38ac0124e49f394250e1877ed89da2022-12-21T20:38:13ZengUniversity of MosulAnnals of the College of Medicine Mosul0027-14462309-62172020-01-0141213914710.33899/mmed.2020.164156164156Helicobacter pylori in Gastric biopsy: A Histochemical and Immunohistochemical AssessmentZainab Waleed Aziz0Shuaib Hashim Saleem1Hatim Abdulmajeed Al-Nuaimy2Department of Pathology, College of Medicine, Ninevah University, Mosul, IraqDepartment of Pathology, College of Medicine, University of Mosul, Mosul, IraqSenior Histopathology , Al-Jumhorii Teaching Hospital, Mosul, Iraq<strong><em>Background:</em></strong> Helicobacter species pylori represent one of the medically prominent and most common infections in the world. Contamination with this microbe has set as a causal factor in the development of gastritis, peptic ulcer, and gastric neoplasia. Consequently, prompt diagnosis is essential. <strong><em>Objectives:</em></strong> This study was conveyed to detect <em>H.pylori</em> in gastric biopsies specimens by using routine Hematoxylin, Modified Giemsa dye as well as immunohistochemical stain, besides to assess the specificity and sensitivity of Helicobacter microbe detection in each method. <strong><em>Patients and methods:</em></strong>The research was both prospective and retrospective, carried out on 100 cases of endoscopically obtained gastric biopsies. Data obtained from archives of the pathology department, at AL-Jamhuri Teaching Hospital, Mosul city, and collected in a period spanning from April 2013 to March 2014. The information included; Age, sex, gastric biopsy location, inflammation status, the presence of dysplasia or carcinoma. <em>Helicobacter pylori</em> infection was assessed histochemically and immunohistochemically. <strong><em>Results:</em></strong> In a total of 100 gastric samples, patients’ age range was 11 to 82 years (mean age of 46.5 years), with a male to female ratio of 1.38:1. <em>Helicobacter pylori</em> bacilli were positive with H&E/MGS in 71 (71%) of cases, increased to 75 (75%) case with IHC. Chronic gastritis noticed in 85 biopsy specimens, 74% were positive for <em>H.pylori</em>. There was a statistically significant difference between IHC and H&E/MGS (p=0.04) for detection of <em>H.pylori.</em> The sensibility and specificity of the H&E/MGS were measured compared with the recommended standard sensitive and specific IHC test; they were 95% and 100% respectively. <strong><em>Conclusion:</em></strong> The routine ancillary stains request for the detection of <em>H.pylori</em> remains a laboratory and an institution right. This study revealed that, in our laboratory, the regular application of ancillary dyes is not obliged for the description of <em>H.pylori</em> because it was readily recognizable in the bulk of sections with haematoxylin staining. However, we recommend the use of IHC in specific circumstances.https://mmed.mosuljournals.com/article_164156_027e6ac88ea244ab92643d8d59001f67.pdfhelicobacter pylorigastric biopsymodified giemsa stainimmunohistochemistrycancer |
| spellingShingle | Zainab Waleed Aziz Shuaib Hashim Saleem Hatim Abdulmajeed Al-Nuaimy Helicobacter pylori in Gastric biopsy: A Histochemical and Immunohistochemical Assessment Annals of the College of Medicine Mosul helicobacter pylori gastric biopsy modified giemsa stain immunohistochemistry cancer |
| title | Helicobacter pylori in Gastric biopsy: A Histochemical and Immunohistochemical Assessment |
| title_full | Helicobacter pylori in Gastric biopsy: A Histochemical and Immunohistochemical Assessment |
| title_fullStr | Helicobacter pylori in Gastric biopsy: A Histochemical and Immunohistochemical Assessment |
| title_full_unstemmed | Helicobacter pylori in Gastric biopsy: A Histochemical and Immunohistochemical Assessment |
| title_short | Helicobacter pylori in Gastric biopsy: A Histochemical and Immunohistochemical Assessment |
| title_sort | helicobacter pylori in gastric biopsy a histochemical and immunohistochemical assessment |
| topic | helicobacter pylori gastric biopsy modified giemsa stain immunohistochemistry cancer |
| url | https://mmed.mosuljournals.com/article_164156_027e6ac88ea244ab92643d8d59001f67.pdf |
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