Reversal of Chemotherapy Resistance to Cisplatin in NSCLC by miRNA-195-5p via Targeting the FGF2 Gene
Hao Wang,1,2 Zhi-lin Sui,1 Xian-xian Wu,1 Peng Tang,1 Hong-dian Zhang,1 Zhen-tao Yu1,3 1Department of Esophageal Cancer, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy of Tianjin City, Tianjin, 300060, People’s Republic of China; 2...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2021-04-01
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| Series: | Pharmacogenomics and Personalized Medicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/reversal-of-chemotherapy-resistance-to-cisplatin-in-nsclc-by-mirna-195-peer-reviewed-fulltext-article-PGPM |
| _version_ | 1818414223103885312 |
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| author | Wang H Sui Z Wu X Tang P Zhang H Yu Z |
| author_facet | Wang H Sui Z Wu X Tang P Zhang H Yu Z |
| author_sort | Wang H |
| collection | DOAJ |
| description | Hao Wang,1,2 Zhi-lin Sui,1 Xian-xian Wu,1 Peng Tang,1 Hong-dian Zhang,1 Zhen-tao Yu1,3 1Department of Esophageal Cancer, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy of Tianjin City, Tianjin, 300060, People’s Republic of China; 2Department of Surgical Oncology, Baotou Cancer Hospital, Baotou, People’s Republic of China; 3Department of Thoracic Surgery, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Shenzhen, 518116, People’s Republic of ChinaCorrespondence: Zhen-tao Yu; Hong-dian ZhangDepartment of Esophageal Cancer, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy of Tianjin City, Tianjin, 300060, People’s Republic of ChinaTel +86 22-23340123Fax +86 22-23359984Email yuzhentao@tjmuch.com; zhdiantjzl@tmu.edu.cnObjective: To explore the mechanism of miR-195-5p in the pathogenesis non-small cell lung cancer (NSCLC) and cisplatin resistance.Methods: The function of miR-195-5p in NSCLC and cisplatin resistance were determined by MTT, scratch assay, transwell assay, and nude mice xenograft experiments. miR-195-5p target gene was identified by dual-luciferase reporter assays and real-time PCR analysis.Results: miR-195-5p content was lower in A549/DDP than that in A549 cells, with reduced chemotherapy sensitivity and increased cell invasion and migration ability. The loss-of-function and gain-of-function assays illustrated that miR-195-5p might have increased the chemosensitivity to cisplatin in the A549/DDP cells and decreased cell migration and invasion. FGF2 is a negatively correlated action target of miR-195-5p. miR-195-5p might affect EMT by inhibiting FGF2. Overexpression of FGF2 resulted in enhanced cisplatin resistance in the cells, while miR-195-5p might have reversed this resistance.Conclusion: Overall, miR-195-5p might target FGF2 to reduce cisplatin resistance in A549/DDP cells and enhance chemosensitivity.Keywords: miR-195-5p, NSCLC, cisplatin, chemosensitivity, FGF2 |
| first_indexed | 2024-12-14T11:15:41Z |
| format | Article |
| id | doaj.art-7fb41e7b2eca48438a24c1b7a2a11f7d |
| institution | Directory Open Access Journal |
| issn | 1178-7066 |
| language | English |
| last_indexed | 2024-12-14T11:15:41Z |
| publishDate | 2021-04-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Pharmacogenomics and Personalized Medicine |
| spelling | doaj.art-7fb41e7b2eca48438a24c1b7a2a11f7d2022-12-21T23:04:03ZengDove Medical PressPharmacogenomics and Personalized Medicine1178-70662021-04-01Volume 1449750864317Reversal of Chemotherapy Resistance to Cisplatin in NSCLC by miRNA-195-5p via Targeting the FGF2 GeneWang HSui ZWu XTang PZhang HYu ZHao Wang,1,2 Zhi-lin Sui,1 Xian-xian Wu,1 Peng Tang,1 Hong-dian Zhang,1 Zhen-tao Yu1,3 1Department of Esophageal Cancer, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy of Tianjin City, Tianjin, 300060, People’s Republic of China; 2Department of Surgical Oncology, Baotou Cancer Hospital, Baotou, People’s Republic of China; 3Department of Thoracic Surgery, National Cancer Center, National Clinical Research Center for Cancer, Cancer Hospital & Shenzhen Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Shenzhen, 518116, People’s Republic of ChinaCorrespondence: Zhen-tao Yu; Hong-dian ZhangDepartment of Esophageal Cancer, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy of Tianjin City, Tianjin, 300060, People’s Republic of ChinaTel +86 22-23340123Fax +86 22-23359984Email yuzhentao@tjmuch.com; zhdiantjzl@tmu.edu.cnObjective: To explore the mechanism of miR-195-5p in the pathogenesis non-small cell lung cancer (NSCLC) and cisplatin resistance.Methods: The function of miR-195-5p in NSCLC and cisplatin resistance were determined by MTT, scratch assay, transwell assay, and nude mice xenograft experiments. miR-195-5p target gene was identified by dual-luciferase reporter assays and real-time PCR analysis.Results: miR-195-5p content was lower in A549/DDP than that in A549 cells, with reduced chemotherapy sensitivity and increased cell invasion and migration ability. The loss-of-function and gain-of-function assays illustrated that miR-195-5p might have increased the chemosensitivity to cisplatin in the A549/DDP cells and decreased cell migration and invasion. FGF2 is a negatively correlated action target of miR-195-5p. miR-195-5p might affect EMT by inhibiting FGF2. Overexpression of FGF2 resulted in enhanced cisplatin resistance in the cells, while miR-195-5p might have reversed this resistance.Conclusion: Overall, miR-195-5p might target FGF2 to reduce cisplatin resistance in A549/DDP cells and enhance chemosensitivity.Keywords: miR-195-5p, NSCLC, cisplatin, chemosensitivity, FGF2https://www.dovepress.com/reversal-of-chemotherapy-resistance-to-cisplatin-in-nsclc-by-mirna-195-peer-reviewed-fulltext-article-PGPMmir-195-5pnsclccisplatin: chemosensitivityfgf2 |
| spellingShingle | Wang H Sui Z Wu X Tang P Zhang H Yu Z Reversal of Chemotherapy Resistance to Cisplatin in NSCLC by miRNA-195-5p via Targeting the FGF2 Gene Pharmacogenomics and Personalized Medicine mir-195-5p nsclc cisplatin: chemosensitivity fgf2 |
| title | Reversal of Chemotherapy Resistance to Cisplatin in NSCLC by miRNA-195-5p via Targeting the FGF2 Gene |
| title_full | Reversal of Chemotherapy Resistance to Cisplatin in NSCLC by miRNA-195-5p via Targeting the FGF2 Gene |
| title_fullStr | Reversal of Chemotherapy Resistance to Cisplatin in NSCLC by miRNA-195-5p via Targeting the FGF2 Gene |
| title_full_unstemmed | Reversal of Chemotherapy Resistance to Cisplatin in NSCLC by miRNA-195-5p via Targeting the FGF2 Gene |
| title_short | Reversal of Chemotherapy Resistance to Cisplatin in NSCLC by miRNA-195-5p via Targeting the FGF2 Gene |
| title_sort | reversal of chemotherapy resistance to cisplatin in nsclc by mirna 195 5p via targeting the fgf2 gene |
| topic | mir-195-5p nsclc cisplatin: chemosensitivity fgf2 |
| url | https://www.dovepress.com/reversal-of-chemotherapy-resistance-to-cisplatin-in-nsclc-by-mirna-195-peer-reviewed-fulltext-article-PGPM |
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