Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, Brazil
Mycobacterium bovis is the causative agent of bovine tuberculosis, a disease that affects dairy herds throughout the Brazilian territory, constituting a neglected zoonosis transmitted by raw milk and its derivatives. In this study, we evaluated the presence of M. bovis and other mycobacteria in Mina...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade de São Paulo
2018-12-01
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| Series: | Brazilian Journal of Veterinary Research and Animal Science |
| Subjects: | |
| Online Access: | http://www.revistas.usp.br/bjvras/article/view/146525 |
| _version_ | 1828409980483534848 |
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| author | Patricia Rossi Moriconi Cássia Yumi Ikuta Fábio Gregori Gisele de Oliveira Sheila de Oliveira Paloma de Oliveira Tonietti José Soares Ferreira Neto Fernando Ferreira Adriana Cortez Evelise Oliveira Telles |
| author_facet | Patricia Rossi Moriconi Cássia Yumi Ikuta Fábio Gregori Gisele de Oliveira Sheila de Oliveira Paloma de Oliveira Tonietti José Soares Ferreira Neto Fernando Ferreira Adriana Cortez Evelise Oliveira Telles |
| author_sort | Patricia Rossi Moriconi |
| collection | DOAJ |
| description | Mycobacterium bovis is the causative agent of bovine tuberculosis, a disease that affects dairy herds throughout the Brazilian territory, constituting a neglected zoonosis transmitted by raw milk and its derivatives. In this study, we evaluated the presence of M. bovis and other mycobacteria in Minas cheese obtained from open fairs in the city of São Paulo between 2012 and 2013. Samples (n = 133) were decontaminated using hexa-cetylpyridinium chloride and seeded on Stonebrink–Leslie medium. The isolates were submitted to molecular identification by TB Multiplex PCR targeting the 16S rRNA gene and amplicon nucleotide sequencing. From 16 cheese samples (12%), we obtained 26 putative colonies of Mycobacterium spp., none of which belonged to any of the Mycobacterium tuberculosis, Mycobacterium avium, or Mycobacterium intracellulare complexes. Phylogenetic analysis showed that sample sequences were grouped in a clade that includes only non-tuberculous mycobacteria with proximity to sequences obtained from Mycobacterium novocastrense (3 sequences), Mycobacterium holsaticum (1 sequence), and
Mycobacterium elephantis (2 sequences). Although no epidemiological evidence was found regarding the importance of oral transmission of mycobacteria in healthy people, their importance in the immunosuppressed population remains uncertain. |
| first_indexed | 2024-12-10T12:04:48Z |
| format | Article |
| id | doaj.art-7fb441279e5948d8939d6c529d10414e |
| institution | Directory Open Access Journal |
| issn | 1413-9596 1678-4456 |
| language | English |
| last_indexed | 2024-12-10T12:04:48Z |
| publishDate | 2018-12-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Veterinary Research and Animal Science |
| spelling | doaj.art-7fb441279e5948d8939d6c529d10414e2022-12-22T01:49:29ZengUniversidade de São PauloBrazilian Journal of Veterinary Research and Animal Science1413-95961678-44562018-12-0155410.11606/issn.1678-4456.bjvras.2018.146525Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, BrazilPatricia Rossi MoriconiCássia Yumi IkutaFábio GregoriGisele de OliveiraSheila de OliveiraPaloma de Oliveira ToniettiJosé Soares Ferreira NetoFernando FerreiraAdriana CortezEvelise Oliveira TellesMycobacterium bovis is the causative agent of bovine tuberculosis, a disease that affects dairy herds throughout the Brazilian territory, constituting a neglected zoonosis transmitted by raw milk and its derivatives. In this study, we evaluated the presence of M. bovis and other mycobacteria in Minas cheese obtained from open fairs in the city of São Paulo between 2012 and 2013. Samples (n = 133) were decontaminated using hexa-cetylpyridinium chloride and seeded on Stonebrink–Leslie medium. The isolates were submitted to molecular identification by TB Multiplex PCR targeting the 16S rRNA gene and amplicon nucleotide sequencing. From 16 cheese samples (12%), we obtained 26 putative colonies of Mycobacterium spp., none of which belonged to any of the Mycobacterium tuberculosis, Mycobacterium avium, or Mycobacterium intracellulare complexes. Phylogenetic analysis showed that sample sequences were grouped in a clade that includes only non-tuberculous mycobacteria with proximity to sequences obtained from Mycobacterium novocastrense (3 sequences), Mycobacterium holsaticum (1 sequence), and Mycobacterium elephantis (2 sequences). Although no epidemiological evidence was found regarding the importance of oral transmission of mycobacteria in healthy people, their importance in the immunosuppressed population remains uncertain.http://www.revistas.usp.br/bjvras/article/view/146525MycobacteriaNon-tuberculous mycobacteriaMycobacterium bovisMinas cheeseFree-trade fair |
| spellingShingle | Patricia Rossi Moriconi Cássia Yumi Ikuta Fábio Gregori Gisele de Oliveira Sheila de Oliveira Paloma de Oliveira Tonietti José Soares Ferreira Neto Fernando Ferreira Adriana Cortez Evelise Oliveira Telles Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, Brazil Brazilian Journal of Veterinary Research and Animal Science Mycobacteria Non-tuberculous mycobacteria Mycobacterium bovis Minas cheese Free-trade fair |
| title | Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, Brazil |
| title_full | Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, Brazil |
| title_fullStr | Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, Brazil |
| title_full_unstemmed | Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, Brazil |
| title_short | Mycobacteria in Minas cheese commercialized in open fairs in São Paulo, Brazil |
| title_sort | mycobacteria in minas cheese commercialized in open fairs in sao paulo brazil |
| topic | Mycobacteria Non-tuberculous mycobacteria Mycobacterium bovis Minas cheese Free-trade fair |
| url | http://www.revistas.usp.br/bjvras/article/view/146525 |
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