Analysis of Antioxidant and Antiviral Effects of Olive (<i>Olea europaea</i> L.) Leaf Extracts and Pure Compound Using Cancer Cell Model
The present study aims to assess the antioxidant and antiviral effectiveness of leaf extracts obtained from <i>Olea europaea</i> L. var. <i>sativa</i> and <i>Olea europaea</i> L. var. <i>sylvestris</i>. The total antioxidant activity was determined via...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/2/238 |
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| author | Rosamaria Pennisi Ichrak Ben Amor Bochra Gargouri Hamadi Attia Rihab Zaabi Ahlem Ben Chira Mongi Saoudi Anna Piperno Paola Trischitta Maria Pia Tamburello Maria Teresa Sciortino |
| author_facet | Rosamaria Pennisi Ichrak Ben Amor Bochra Gargouri Hamadi Attia Rihab Zaabi Ahlem Ben Chira Mongi Saoudi Anna Piperno Paola Trischitta Maria Pia Tamburello Maria Teresa Sciortino |
| author_sort | Rosamaria Pennisi |
| collection | DOAJ |
| description | The present study aims to assess the antioxidant and antiviral effectiveness of leaf extracts obtained from <i>Olea europaea</i> L. var. <i>sativa</i> and <i>Olea europaea</i> L. var. <i>sylvestris</i>. The total antioxidant activity was determined via both an ammonium phosphomolybdate assay and a nitric oxide radical inhibition assay. Both extracts showed reducing abilities in an in vitro system and in human HeLa cells. Indeed, after oxidative stress induction, we found that exposition to olive leaf extracts protects human HeLa cells from lipid peroxidation and increases the concentration of enzyme antioxidants such as catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase. Additionally, OESA treatment affects viral DNA accumulation more than OESY, probably due to the exclusive oleuropein content. In fact, subtoxic concentrations of oleuropein inhibit HSV-1 replication, stimulating the phosphorylation of PKR, c-FOS, and c-JUN proteins. These results provide new knowledge about the potential health benefits and mechanisms of action of oleuropein and oleuropein-rich extracts. |
| first_indexed | 2024-03-11T09:06:02Z |
| format | Article |
| id | doaj.art-7fb52faa3ebf48598ebc45e89d78abe0 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T09:06:02Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-7fb52faa3ebf48598ebc45e89d78abe02023-11-16T19:22:18ZengMDPI AGBiomolecules2218-273X2023-01-0113223810.3390/biom13020238Analysis of Antioxidant and Antiviral Effects of Olive (<i>Olea europaea</i> L.) Leaf Extracts and Pure Compound Using Cancer Cell ModelRosamaria Pennisi0Ichrak Ben Amor1Bochra Gargouri2Hamadi Attia3Rihab Zaabi4Ahlem Ben Chira5Mongi Saoudi6Anna Piperno7Paola Trischitta8Maria Pia Tamburello9Maria Teresa Sciortino10Department of Chemical, Biological, Pharmaceutical, and Environmental Sciences, University of Messina, Viale SS. Annunziata, 98168 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical, and Environmental Sciences, University of Messina, Viale SS. Annunziata, 98168 Messina, ItalyUnit of Biotechnology and Pathologies, Higher Institute of Biotechnology of Sfax, University of Sfax, Sfax 3029, TunisiaUnit of Biotechnology and Pathologies, Higher Institute of Biotechnology of Sfax, University of Sfax, Sfax 3029, TunisiaUnit of Biotechnology and Pathologies, Higher Institute of Biotechnology of Sfax, University of Sfax, Sfax 3029, TunisiaAnimal Ecophysiology Laboratory, Sciences Faculty of Sfax, University of Sfax, Sfax 3054, TunisiaAnimal Ecophysiology Laboratory, Sciences Faculty of Sfax, University of Sfax, Sfax 3054, TunisiaDepartment of Chemical, Biological, Pharmaceutical, and Environmental Sciences, University of Messina, Viale SS. Annunziata, 98168 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical, and Environmental Sciences, University of Messina, Viale SS. Annunziata, 98168 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical, and Environmental Sciences, University of Messina, Viale SS. Annunziata, 98168 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical, and Environmental Sciences, University of Messina, Viale SS. Annunziata, 98168 Messina, ItalyThe present study aims to assess the antioxidant and antiviral effectiveness of leaf extracts obtained from <i>Olea europaea</i> L. var. <i>sativa</i> and <i>Olea europaea</i> L. var. <i>sylvestris</i>. The total antioxidant activity was determined via both an ammonium phosphomolybdate assay and a nitric oxide radical inhibition assay. Both extracts showed reducing abilities in an in vitro system and in human HeLa cells. Indeed, after oxidative stress induction, we found that exposition to olive leaf extracts protects human HeLa cells from lipid peroxidation and increases the concentration of enzyme antioxidants such as catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase. Additionally, OESA treatment affects viral DNA accumulation more than OESY, probably due to the exclusive oleuropein content. In fact, subtoxic concentrations of oleuropein inhibit HSV-1 replication, stimulating the phosphorylation of PKR, c-FOS, and c-JUN proteins. These results provide new knowledge about the potential health benefits and mechanisms of action of oleuropein and oleuropein-rich extracts.https://www.mdpi.com/2218-273X/13/2/238<i>Olea europaea</i> L.oleuropeinoxidative stressantiviral activityherpes simplex virus 1innate immune response |
| spellingShingle | Rosamaria Pennisi Ichrak Ben Amor Bochra Gargouri Hamadi Attia Rihab Zaabi Ahlem Ben Chira Mongi Saoudi Anna Piperno Paola Trischitta Maria Pia Tamburello Maria Teresa Sciortino Analysis of Antioxidant and Antiviral Effects of Olive (<i>Olea europaea</i> L.) Leaf Extracts and Pure Compound Using Cancer Cell Model Biomolecules <i>Olea europaea</i> L. oleuropein oxidative stress antiviral activity herpes simplex virus 1 innate immune response |
| title | Analysis of Antioxidant and Antiviral Effects of Olive (<i>Olea europaea</i> L.) Leaf Extracts and Pure Compound Using Cancer Cell Model |
| title_full | Analysis of Antioxidant and Antiviral Effects of Olive (<i>Olea europaea</i> L.) Leaf Extracts and Pure Compound Using Cancer Cell Model |
| title_fullStr | Analysis of Antioxidant and Antiviral Effects of Olive (<i>Olea europaea</i> L.) Leaf Extracts and Pure Compound Using Cancer Cell Model |
| title_full_unstemmed | Analysis of Antioxidant and Antiviral Effects of Olive (<i>Olea europaea</i> L.) Leaf Extracts and Pure Compound Using Cancer Cell Model |
| title_short | Analysis of Antioxidant and Antiviral Effects of Olive (<i>Olea europaea</i> L.) Leaf Extracts and Pure Compound Using Cancer Cell Model |
| title_sort | analysis of antioxidant and antiviral effects of olive i olea europaea i l leaf extracts and pure compound using cancer cell model |
| topic | <i>Olea europaea</i> L. oleuropein oxidative stress antiviral activity herpes simplex virus 1 innate immune response |
| url | https://www.mdpi.com/2218-273X/13/2/238 |
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