Changes in adipose tissue lipolysis gene expression and insulin sensitivity after weight loss
Objective: Insulin resistance is a major pathophysiological link between obesity and its metabolic complications. Weight loss (WL) is an effective tool t o prevent obesity-related diseases; however, the mechanisms of an improvement in insulin sensitivity (IS) after weight-reducing interventions are...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Bioscientifica
2020-02-01
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| Series: | Endocrine Connections |
| Subjects: | |
| Online Access: | https://ec.bioscientifica.com/view/journals/ec/9/2/EC-19-0507.xml |
| Summary: | Objective: Insulin resistance is a major pathophysiological link between obesity and its metabolic complications. Weight loss (WL) is an effective tool t o prevent obesity-related diseases; however, the mechanisms of an improvement in insulin sensitivity (IS) after weight-reducing interventions are not completely understood. The aim of the present study was to analyze the relationships between IS and adipose t issue (AT) expression of the genes involved in the regulation of lipolysis in obese subj ects after WL.
Methods: Fifty-two obese subjects underwent weight-reducing dietary int ervention program. The control group comprised 20 normal-weight subjects, examined at baseline only. Hyperinsulinemic-euglycemic clamp and s.c. AT biopsy with subsequent gene expression analysis were performed before and after the program .
Results: AT expression of genes encoding lipases (PNPLA2, LIPE and MGLL) and lipid-droplet proteins enhancing (ABHD5) and inhibiting lipolysis (PLIN1 and CIDEA) were decreased in obese individuals in comparison with normal-weight individuals. The group of 38 obese participants completed dietary intervention program and clamp studies, which resulted in a significant WL and an improvement in mean IS . However, in nine subjects from this group IS did not improve in response to WL. AT expression of PNPLA2, LIPE and PLIN1 increased only in the group without IS improvement.
Conclusions: Excessive lipolysis may prevent an improvement in IS during WL . The change in AT PNPLA2 and LIPE expression was a negative predictor of the change in IS after WL. |
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| ISSN: | 2049-3614 2049-3614 |