Abnormal Changes of Monocyte Subsets in Patients With Sjögren’s Syndrome
BackgroundRecent studies have proven the existence of distinct monocyte subsets, which play a significant role in the development of some rheumatic diseases such as systemic lupus erythematosus (SLE). This study was performed to define the changes of monocyte subsets in patients with Sjögren’s Syndr...
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.864920/full |
| _version_ | 1818487615716851712 |
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| author | Yan He Yan He Rongjuan Chen Rongjuan Chen Mengqin Zhang Mengqin Zhang Bin Wang Bin Wang Zhangdi Liao Zhangdi Liao Guixiu Shi Guixiu Shi Yan Li Yan Li |
| author_facet | Yan He Yan He Rongjuan Chen Rongjuan Chen Mengqin Zhang Mengqin Zhang Bin Wang Bin Wang Zhangdi Liao Zhangdi Liao Guixiu Shi Guixiu Shi Yan Li Yan Li |
| author_sort | Yan He |
| collection | DOAJ |
| description | BackgroundRecent studies have proven the existence of distinct monocyte subsets, which play a significant role in the development of some rheumatic diseases such as systemic lupus erythematosus (SLE). This study was performed to define the changes of monocyte subsets in patients with Sjögren’s Syndrome (SjS).MethodsSingle cell RNA-sequencing (scRNA-seq) data of monocytes from SjS patients and controls were analyzed. The transcriptomic changes in monocyte subsets between SjS and controls were identified and potential key functional pathways involved in SjS development were also explored.ResultsA total of 11 monocyte subsets were identified in the scRNA-seq analyses of monocytes. A new monocyte subset characterized by higher expression of VNN2 (GPI-80) and S100A12 (Monocyte cluster 3) was identified, and it was increased in SjS patients. Compared with controls, almost all monocyte subsets from SjS patients had increased expression of TNFSF10 (TRAIL). Moreover, interferon (IFN)-related and neutrophil activation-associated pathways were main up-regulated pathways in the monocytes of SjS patients.ConclusionThis study uncovered the abnormal changes in monocyte subsets and their transcriptomic changes in SjS patients, and identified TNFSF10 high/+ monocytes as a potential key player in SjS pathogenesis and a promising target for SjS treatment. |
| first_indexed | 2024-12-10T16:40:24Z |
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| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-10T16:40:24Z |
| publishDate | 2022-03-01 |
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| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-7fc0329664f242eab50a77f2b956f6f32022-12-22T01:41:15ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-03-011310.3389/fimmu.2022.864920864920Abnormal Changes of Monocyte Subsets in Patients With Sjögren’s SyndromeYan He0Yan He1Rongjuan Chen2Rongjuan Chen3Mengqin Zhang4Mengqin Zhang5Bin Wang6Bin Wang7Zhangdi Liao8Zhangdi Liao9Guixiu Shi10Guixiu Shi11Yan Li12Yan Li13Department of Rheumatology and Clinical Immunology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Rheumatology and Clinical Immunology, Xiamen Science and Technology Bureau, Xiamen, ChinaDepartment of Rheumatology and Clinical Immunology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Rheumatology and Clinical Immunology, Xiamen Science and Technology Bureau, Xiamen, ChinaDepartment of Rheumatology and Clinical Immunology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Rheumatology and Clinical Immunology, Xiamen Science and Technology Bureau, Xiamen, ChinaDepartment of Rheumatology and Clinical Immunology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Rheumatology and Clinical Immunology, Xiamen Science and Technology Bureau, Xiamen, ChinaDepartment of Rheumatology and Clinical Immunology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Rheumatology and Clinical Immunology, Xiamen Science and Technology Bureau, Xiamen, ChinaDepartment of Rheumatology and Clinical Immunology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Rheumatology and Clinical Immunology, Xiamen Science and Technology Bureau, Xiamen, ChinaDepartment of Rheumatology and Clinical Immunology, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, ChinaXiamen Key Laboratory of Rheumatology and Clinical Immunology, Xiamen Science and Technology Bureau, Xiamen, ChinaBackgroundRecent studies have proven the existence of distinct monocyte subsets, which play a significant role in the development of some rheumatic diseases such as systemic lupus erythematosus (SLE). This study was performed to define the changes of monocyte subsets in patients with Sjögren’s Syndrome (SjS).MethodsSingle cell RNA-sequencing (scRNA-seq) data of monocytes from SjS patients and controls were analyzed. The transcriptomic changes in monocyte subsets between SjS and controls were identified and potential key functional pathways involved in SjS development were also explored.ResultsA total of 11 monocyte subsets were identified in the scRNA-seq analyses of monocytes. A new monocyte subset characterized by higher expression of VNN2 (GPI-80) and S100A12 (Monocyte cluster 3) was identified, and it was increased in SjS patients. Compared with controls, almost all monocyte subsets from SjS patients had increased expression of TNFSF10 (TRAIL). Moreover, interferon (IFN)-related and neutrophil activation-associated pathways were main up-regulated pathways in the monocytes of SjS patients.ConclusionThis study uncovered the abnormal changes in monocyte subsets and their transcriptomic changes in SjS patients, and identified TNFSF10 high/+ monocytes as a potential key player in SjS pathogenesis and a promising target for SjS treatment.https://www.frontiersin.org/articles/10.3389/fimmu.2022.864920/fullSjögren’s syndromemonocyte subsetssingle cell RNA-sequencingpathogenesistranscriptomic analyses |
| spellingShingle | Yan He Yan He Rongjuan Chen Rongjuan Chen Mengqin Zhang Mengqin Zhang Bin Wang Bin Wang Zhangdi Liao Zhangdi Liao Guixiu Shi Guixiu Shi Yan Li Yan Li Abnormal Changes of Monocyte Subsets in Patients With Sjögren’s Syndrome Frontiers in Immunology Sjögren’s syndrome monocyte subsets single cell RNA-sequencing pathogenesis transcriptomic analyses |
| title | Abnormal Changes of Monocyte Subsets in Patients With Sjögren’s Syndrome |
| title_full | Abnormal Changes of Monocyte Subsets in Patients With Sjögren’s Syndrome |
| title_fullStr | Abnormal Changes of Monocyte Subsets in Patients With Sjögren’s Syndrome |
| title_full_unstemmed | Abnormal Changes of Monocyte Subsets in Patients With Sjögren’s Syndrome |
| title_short | Abnormal Changes of Monocyte Subsets in Patients With Sjögren’s Syndrome |
| title_sort | abnormal changes of monocyte subsets in patients with sjogren s syndrome |
| topic | Sjögren’s syndrome monocyte subsets single cell RNA-sequencing pathogenesis transcriptomic analyses |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.864920/full |
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