The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model
Abstract The pituitary gland plays an important endocrinal role, however its damage after cardiac arrest (CA) has not been well elucidated. The aim of this study was to determine a pituitary gland damage induced by CA. Rats were subjected to 10-min asphyxia and cardiopulmonary resuscitation (CPR). I...
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| Format: | Article |
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Nature Portfolio
2021-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-79780-3 |
| _version_ | 1818346211547021312 |
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| author | Yu Okuma Tomoaki Aoki Santiago J. Miyara Kei Hayashida Mitsuaki Nishikimi Ryosuke Takegawa Tai Yin Junhwan Kim Lance B. Becker Koichiro Shinozaki |
| author_facet | Yu Okuma Tomoaki Aoki Santiago J. Miyara Kei Hayashida Mitsuaki Nishikimi Ryosuke Takegawa Tai Yin Junhwan Kim Lance B. Becker Koichiro Shinozaki |
| author_sort | Yu Okuma |
| collection | DOAJ |
| description | Abstract The pituitary gland plays an important endocrinal role, however its damage after cardiac arrest (CA) has not been well elucidated. The aim of this study was to determine a pituitary gland damage induced by CA. Rats were subjected to 10-min asphyxia and cardiopulmonary resuscitation (CPR). Immunohistochemistry and ELISA assays were used to evaluate the pituitary damage and endocrine function. Samples were collected at pre-CA, and 30 and 120 min after cardio pulmonary resuscitation. Triphenyltetrazolium chloride (TTC) staining demonstrated the expansion of the pituitary damage over time. There was phenotypic validity between the pars distalis and nervosa. Both CT-proAVP (pars nervosa hormone) and GH/IGF-1 (pars distalis hormone) decreased over time, and a different expression pattern corresponding to the damaged areas was noted (CT-proAVP, 30.2 ± 6.2, 31.5 ± 5.9, and 16.3 ± 7.6 pg/mg protein, p < 0.01; GH/IGF-1, 2.63 ± 0.61, 0.62 ± 0.36, and 2.01 ± 0.41 ng/mg protein, p < 0.01 respectively). Similarly, the expression pattern between these hormones in the end-organ systems showed phenotypic validity. Plasma CT-proAVP (r = 0.771, p = 0.025) and IGF-1 (r = −0.775, p = 0.024) demonstrated a strong correlation with TTC staining area. Our data suggested that CA induces pathological and functional damage to the pituitary gland. |
| first_indexed | 2024-12-13T17:14:40Z |
| format | Article |
| id | doaj.art-7fc1625c4de34b6c93af84813e35c18c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-13T17:14:40Z |
| publishDate | 2021-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7fc1625c4de34b6c93af84813e35c18c2022-12-21T23:37:27ZengNature PortfolioScientific Reports2045-23222021-01-0111111210.1038/s41598-020-79780-3The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent modelYu Okuma0Tomoaki Aoki1Santiago J. Miyara2Kei Hayashida3Mitsuaki Nishikimi4Ryosuke Takegawa5Tai Yin6Junhwan Kim7Lance B. Becker8Koichiro Shinozaki9The Feinstein Institutes for Medical Research, Northwell HealthThe Feinstein Institutes for Medical Research, Northwell HealthThe Feinstein Institutes for Medical Research, Northwell HealthThe Feinstein Institutes for Medical Research, Northwell HealthThe Feinstein Institutes for Medical Research, Northwell HealthThe Feinstein Institutes for Medical Research, Northwell HealthThe Feinstein Institutes for Medical Research, Northwell HealthThe Feinstein Institutes for Medical Research, Northwell HealthThe Feinstein Institutes for Medical Research, Northwell HealthThe Feinstein Institutes for Medical Research, Northwell HealthAbstract The pituitary gland plays an important endocrinal role, however its damage after cardiac arrest (CA) has not been well elucidated. The aim of this study was to determine a pituitary gland damage induced by CA. Rats were subjected to 10-min asphyxia and cardiopulmonary resuscitation (CPR). Immunohistochemistry and ELISA assays were used to evaluate the pituitary damage and endocrine function. Samples were collected at pre-CA, and 30 and 120 min after cardio pulmonary resuscitation. Triphenyltetrazolium chloride (TTC) staining demonstrated the expansion of the pituitary damage over time. There was phenotypic validity between the pars distalis and nervosa. Both CT-proAVP (pars nervosa hormone) and GH/IGF-1 (pars distalis hormone) decreased over time, and a different expression pattern corresponding to the damaged areas was noted (CT-proAVP, 30.2 ± 6.2, 31.5 ± 5.9, and 16.3 ± 7.6 pg/mg protein, p < 0.01; GH/IGF-1, 2.63 ± 0.61, 0.62 ± 0.36, and 2.01 ± 0.41 ng/mg protein, p < 0.01 respectively). Similarly, the expression pattern between these hormones in the end-organ systems showed phenotypic validity. Plasma CT-proAVP (r = 0.771, p = 0.025) and IGF-1 (r = −0.775, p = 0.024) demonstrated a strong correlation with TTC staining area. Our data suggested that CA induces pathological and functional damage to the pituitary gland.https://doi.org/10.1038/s41598-020-79780-3 |
| spellingShingle | Yu Okuma Tomoaki Aoki Santiago J. Miyara Kei Hayashida Mitsuaki Nishikimi Ryosuke Takegawa Tai Yin Junhwan Kim Lance B. Becker Koichiro Shinozaki The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model Scientific Reports |
| title | The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model |
| title_full | The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model |
| title_fullStr | The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model |
| title_full_unstemmed | The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model |
| title_short | The evaluation of pituitary damage associated with cardiac arrest: An experimental rodent model |
| title_sort | evaluation of pituitary damage associated with cardiac arrest an experimental rodent model |
| url | https://doi.org/10.1038/s41598-020-79780-3 |
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