Amplicon-based skin microbiome profiles collected by tape stripping with different adhesive film dressings: a comparative study
Abstract Background Medical film dressings have been used to obtain skin microbiota for skin microbiome studies, although their adhesive force may be so strong that the skin could be injured when applied to those who have fragile skin, such as older people. Several products with less adhesive force...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-02-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-021-02122-4 |
| _version_ | 1818326030356578304 |
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| author | Kazuhiro Ogai Kana Shibata Natsuki Takahashi Kohei Ogura Shigefumi Okamoto Junko Sugama |
| author_facet | Kazuhiro Ogai Kana Shibata Natsuki Takahashi Kohei Ogura Shigefumi Okamoto Junko Sugama |
| author_sort | Kazuhiro Ogai |
| collection | DOAJ |
| description | Abstract Background Medical film dressings have been used to obtain skin microbiota for skin microbiome studies, although their adhesive force may be so strong that the skin could be injured when applied to those who have fragile skin, such as older people. Several products with less adhesive force are available, although their applicability for skin microbiome studies remains unknown. This study aimed to test whether the dressings with less adhesive force could be used for amplicon-based skin microbiome studies. A set of three different film dressings, with acrylic, urethane, or silicone adhesive, was applied to the back skin of nine healthy young participants. The copy number of the 16S ribosomal RNA (rRNA) gene, microbial compositions, and alpha and beta diversity indices were analyzed by amplicon analysis of the 16S rRNA gene using next-generation sequencing and were compared among the three film dressings. Results The dressing with acrylic adhesive yielded the highest copy number of 16S rRNA genes, followed by that with urethane adhesive. The silicone-adhesive dressing yielded a significantly lower copy number of the 16S rRNA gene. The microbial composition of skin microbiota was similar among the three film dressings, although significant differences in the relative abundance of Pseudomonas species and alpha diversity indices were found in the silicone-adhesive dressing. The Bray–Curtis dissimilarity was significantly higher between the acrylic- and silicone-adhesive dressings than between the acrylic- and urethane-adhesive dressings. No adverse effects related to tape stripping were observed for any of the film dressings. Conclusion We recommend dressings with acrylic or urethane adhesive for amplicon-based skin microbiome studies. An acrylic adhesive has an advantage in the yield of skin microbiota, and a urethane adhesive should be chosen when applied to fragile skin. The adhesive force of the dressing with silicone adhesive was too weak to be used for collecting skin microbiota. |
| first_indexed | 2024-12-13T11:53:53Z |
| format | Article |
| id | doaj.art-7fc21da9242e4e0c95a73d9a1b030554 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-13T11:53:53Z |
| publishDate | 2021-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-7fc21da9242e4e0c95a73d9a1b0305542022-12-21T23:47:16ZengBMCBMC Microbiology1471-21802021-02-012111910.1186/s12866-021-02122-4Amplicon-based skin microbiome profiles collected by tape stripping with different adhesive film dressings: a comparative studyKazuhiro Ogai0Kana Shibata1Natsuki Takahashi2Kohei Ogura3Shigefumi Okamoto4Junko Sugama5AI Hospital/Macro Signal Dynamics Research and Development Center, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa UniversityDepartment of Clinical Laboratory Science, Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa UniversityDepartment of Clinical Laboratory Science, Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa UniversityAdvanced Health Care Science Research Unit, Institute for Frontier Science Initiative, Kanazawa UniversityDepartment of Clinical Laboratory Science, Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa UniversityDepartment of Nursing, Faculty of Health Sciences, Institute of Medical, Pharmaceutical and Health Sciences, Kanazawa UniversityAbstract Background Medical film dressings have been used to obtain skin microbiota for skin microbiome studies, although their adhesive force may be so strong that the skin could be injured when applied to those who have fragile skin, such as older people. Several products with less adhesive force are available, although their applicability for skin microbiome studies remains unknown. This study aimed to test whether the dressings with less adhesive force could be used for amplicon-based skin microbiome studies. A set of three different film dressings, with acrylic, urethane, or silicone adhesive, was applied to the back skin of nine healthy young participants. The copy number of the 16S ribosomal RNA (rRNA) gene, microbial compositions, and alpha and beta diversity indices were analyzed by amplicon analysis of the 16S rRNA gene using next-generation sequencing and were compared among the three film dressings. Results The dressing with acrylic adhesive yielded the highest copy number of 16S rRNA genes, followed by that with urethane adhesive. The silicone-adhesive dressing yielded a significantly lower copy number of the 16S rRNA gene. The microbial composition of skin microbiota was similar among the three film dressings, although significant differences in the relative abundance of Pseudomonas species and alpha diversity indices were found in the silicone-adhesive dressing. The Bray–Curtis dissimilarity was significantly higher between the acrylic- and silicone-adhesive dressings than between the acrylic- and urethane-adhesive dressings. No adverse effects related to tape stripping were observed for any of the film dressings. Conclusion We recommend dressings with acrylic or urethane adhesive for amplicon-based skin microbiome studies. An acrylic adhesive has an advantage in the yield of skin microbiota, and a urethane adhesive should be chosen when applied to fragile skin. The adhesive force of the dressing with silicone adhesive was too weak to be used for collecting skin microbiota.https://doi.org/10.1186/s12866-021-02122-4SkinMicrobiomeTape-strippingAdhesive16S rRNANext generation sequencing |
| spellingShingle | Kazuhiro Ogai Kana Shibata Natsuki Takahashi Kohei Ogura Shigefumi Okamoto Junko Sugama Amplicon-based skin microbiome profiles collected by tape stripping with different adhesive film dressings: a comparative study BMC Microbiology Skin Microbiome Tape-stripping Adhesive 16S rRNA Next generation sequencing |
| title | Amplicon-based skin microbiome profiles collected by tape stripping with different adhesive film dressings: a comparative study |
| title_full | Amplicon-based skin microbiome profiles collected by tape stripping with different adhesive film dressings: a comparative study |
| title_fullStr | Amplicon-based skin microbiome profiles collected by tape stripping with different adhesive film dressings: a comparative study |
| title_full_unstemmed | Amplicon-based skin microbiome profiles collected by tape stripping with different adhesive film dressings: a comparative study |
| title_short | Amplicon-based skin microbiome profiles collected by tape stripping with different adhesive film dressings: a comparative study |
| title_sort | amplicon based skin microbiome profiles collected by tape stripping with different adhesive film dressings a comparative study |
| topic | Skin Microbiome Tape-stripping Adhesive 16S rRNA Next generation sequencing |
| url | https://doi.org/10.1186/s12866-021-02122-4 |
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