Antimicrobial Materials with Lime Oil and a Poly(3-hydroxyalkanoate) Produced via Valorisation of Sugar Cane Molasses
A medium chain-length polyhydroxyalkanoate (PHA) was produced by <i>Pseudomonas mendocina</i> CH50 using a cheap carbon substrate, sugarcane molasses. A PHA yield of 14.2% dry cell weight was achieved. Chemical analysis confirmed that the polymer produced was a medium chain-length PHA, a...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-04-01
|
| Series: | Journal of Functional Biomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4983/11/2/24 |
| _version_ | 1797571043244638208 |
|---|---|
| author | Pooja Basnett Elena Marcello Barbara Lukasiewicz Rinat Nigmatullin Alexandra Paxinou Muhammad Haseeb Ahmad Bhavana Gurumayum Ipsita Roy |
| author_facet | Pooja Basnett Elena Marcello Barbara Lukasiewicz Rinat Nigmatullin Alexandra Paxinou Muhammad Haseeb Ahmad Bhavana Gurumayum Ipsita Roy |
| author_sort | Pooja Basnett |
| collection | DOAJ |
| description | A medium chain-length polyhydroxyalkanoate (PHA) was produced by <i>Pseudomonas mendocina</i> CH50 using a cheap carbon substrate, sugarcane molasses. A PHA yield of 14.2% dry cell weight was achieved. Chemical analysis confirmed that the polymer produced was a medium chain-length PHA, a copolymer of 3-hydroxyoctanoate and 3-hydroxydecanoate, P(3HO-<i>co</i>-3HD). Lime oil, an essential oil with known antimicrobial activity, was used as an additive to P(3HO-<i>co</i>-3HD) to confer antibacterial properties to this biodegradable polymer. The incorporation of lime oil induced a slight decrease in crystallinity of P(3HO-co-3HD) films. The antibacterial properties of lime oil were investigated using ISO 20776 against <i>Staphylococcus aureus</i> 6538P and <i>Escherichia coli</i> 8739, showing a higher activity against the Gram-positive bacteria. The higher activity of the oil against <i>S. aureus</i> 6538P defined the higher efficiency of loaded polymer films against this strain. The effect of storage on the antimicrobial properties of the loaded films was investigated. After one-year storage, the content of lime oil in the films decreased, causing a reduction of the antimicrobial activity of the materials produced. However, the films still possessed antibacterial activity against <i>S. aureus</i> 6538P. |
| first_indexed | 2024-03-10T20:33:59Z |
| format | Article |
| id | doaj.art-7fc51db2fcbe4b6494a92e9424837a1d |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-10T20:33:59Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-7fc51db2fcbe4b6494a92e9424837a1d2023-11-19T21:11:23ZengMDPI AGJournal of Functional Biomaterials2079-49832020-04-011122410.3390/jfb11020024Antimicrobial Materials with Lime Oil and a Poly(3-hydroxyalkanoate) Produced via Valorisation of Sugar Cane MolassesPooja Basnett0Elena Marcello1Barbara Lukasiewicz2Rinat Nigmatullin3Alexandra Paxinou4Muhammad Haseeb Ahmad5Bhavana Gurumayum6Ipsita Roy7Faculty of Science and Technology, University of Westminster, London W1W 6UW, UKFaculty of Science and Technology, University of Westminster, London W1W 6UW, UKFaculty of Science and Technology, University of Westminster, London W1W 6UW, UKFaculty of Science and Technology, University of Westminster, London W1W 6UW, UKFaculty of Science and Technology, University of Westminster, London W1W 6UW, UKFaculty of Science and Technology, University of Westminster, London W1W 6UW, UKFaculty of Science and Technology, University of Westminster, London W1W 6UW, UKDepartment of Material Science and Engineering, Faculty of Engineering, University of Sheffield, Sheffield S1 3JD, UKA medium chain-length polyhydroxyalkanoate (PHA) was produced by <i>Pseudomonas mendocina</i> CH50 using a cheap carbon substrate, sugarcane molasses. A PHA yield of 14.2% dry cell weight was achieved. Chemical analysis confirmed that the polymer produced was a medium chain-length PHA, a copolymer of 3-hydroxyoctanoate and 3-hydroxydecanoate, P(3HO-<i>co</i>-3HD). Lime oil, an essential oil with known antimicrobial activity, was used as an additive to P(3HO-<i>co</i>-3HD) to confer antibacterial properties to this biodegradable polymer. The incorporation of lime oil induced a slight decrease in crystallinity of P(3HO-co-3HD) films. The antibacterial properties of lime oil were investigated using ISO 20776 against <i>Staphylococcus aureus</i> 6538P and <i>Escherichia coli</i> 8739, showing a higher activity against the Gram-positive bacteria. The higher activity of the oil against <i>S. aureus</i> 6538P defined the higher efficiency of loaded polymer films against this strain. The effect of storage on the antimicrobial properties of the loaded films was investigated. After one-year storage, the content of lime oil in the films decreased, causing a reduction of the antimicrobial activity of the materials produced. However, the films still possessed antibacterial activity against <i>S. aureus</i> 6538P.https://www.mdpi.com/2079-4983/11/2/24polyhydroxyalkanotessugarcane molassesantibacterial materialsessential oils |
| spellingShingle | Pooja Basnett Elena Marcello Barbara Lukasiewicz Rinat Nigmatullin Alexandra Paxinou Muhammad Haseeb Ahmad Bhavana Gurumayum Ipsita Roy Antimicrobial Materials with Lime Oil and a Poly(3-hydroxyalkanoate) Produced via Valorisation of Sugar Cane Molasses Journal of Functional Biomaterials polyhydroxyalkanotes sugarcane molasses antibacterial materials essential oils |
| title | Antimicrobial Materials with Lime Oil and a Poly(3-hydroxyalkanoate) Produced via Valorisation of Sugar Cane Molasses |
| title_full | Antimicrobial Materials with Lime Oil and a Poly(3-hydroxyalkanoate) Produced via Valorisation of Sugar Cane Molasses |
| title_fullStr | Antimicrobial Materials with Lime Oil and a Poly(3-hydroxyalkanoate) Produced via Valorisation of Sugar Cane Molasses |
| title_full_unstemmed | Antimicrobial Materials with Lime Oil and a Poly(3-hydroxyalkanoate) Produced via Valorisation of Sugar Cane Molasses |
| title_short | Antimicrobial Materials with Lime Oil and a Poly(3-hydroxyalkanoate) Produced via Valorisation of Sugar Cane Molasses |
| title_sort | antimicrobial materials with lime oil and a poly 3 hydroxyalkanoate produced via valorisation of sugar cane molasses |
| topic | polyhydroxyalkanotes sugarcane molasses antibacterial materials essential oils |
| url | https://www.mdpi.com/2079-4983/11/2/24 |
| work_keys_str_mv | AT poojabasnett antimicrobialmaterialswithlimeoilandapoly3hydroxyalkanoateproducedviavalorisationofsugarcanemolasses AT elenamarcello antimicrobialmaterialswithlimeoilandapoly3hydroxyalkanoateproducedviavalorisationofsugarcanemolasses AT barbaralukasiewicz antimicrobialmaterialswithlimeoilandapoly3hydroxyalkanoateproducedviavalorisationofsugarcanemolasses AT rinatnigmatullin antimicrobialmaterialswithlimeoilandapoly3hydroxyalkanoateproducedviavalorisationofsugarcanemolasses AT alexandrapaxinou antimicrobialmaterialswithlimeoilandapoly3hydroxyalkanoateproducedviavalorisationofsugarcanemolasses AT muhammadhaseebahmad antimicrobialmaterialswithlimeoilandapoly3hydroxyalkanoateproducedviavalorisationofsugarcanemolasses AT bhavanagurumayum antimicrobialmaterialswithlimeoilandapoly3hydroxyalkanoateproducedviavalorisationofsugarcanemolasses AT ipsitaroy antimicrobialmaterialswithlimeoilandapoly3hydroxyalkanoateproducedviavalorisationofsugarcanemolasses |