DNA Barcoding of Marine Mollusks Associated with <i>Corallina officinalis</i> Turfs in Southern Istria (Adriatic Sea)
Presence of mollusk assemblages was studied within red coralligenous algae <i>Corallina officinalis</i> L. along the southern Istrian coast. <i>C. officinalis</i> turfs can be considered a biodiversity reservoir, as they shelter numerous invertebrate species. The aim of this...
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MDPI AG
2021-04-01
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| author | Moira Buršić Ljiljana Iveša Andrej Jaklin Milvana Arko Pijevac Mladen Kučinić Mauro Štifanić Lucija Neal Branka Bruvo Mađarić |
| author_facet | Moira Buršić Ljiljana Iveša Andrej Jaklin Milvana Arko Pijevac Mladen Kučinić Mauro Štifanić Lucija Neal Branka Bruvo Mađarić |
| author_sort | Moira Buršić |
| collection | DOAJ |
| description | Presence of mollusk assemblages was studied within red coralligenous algae <i>Corallina officinalis</i> L. along the southern Istrian coast. <i>C. officinalis</i> turfs can be considered a biodiversity reservoir, as they shelter numerous invertebrate species. The aim of this study was to identify mollusk species within these settlements using DNA barcoding as a method for detailed identification of mollusks. Nine locations and 18 localities with algal coverage range above 90% were chosen at four research areas. From 54 collected samples of <i>C. officinalis</i> turfs, a total of 46 mollusk species were identified. Molecular methods helped identify 16 gastropod, 14 bivalve and one polyplacophoran species. COI sequences for two bivalve species (<i>Musculus</i> cf. <i>costulatus</i> (Risso, 1826) and <i>Gregariella semigranata</i> (Reeve, 1858)) and seven gastropod species (<i>Megastomia winfriedi</i> Peñas & Rolán, 1999, <i>Eatonina</i> sp. Thiele, 1912, <i>Eatonina cossurae</i> (Calcara, 1841), <i>Crisilla</i> cf. <i>maculata</i> (Monterosato, 1869), <i>Alvania</i> cf. <i>carinata</i> (da Costa, 1778), <i>Vitreolina antiflexa</i> (Monterosato, 1884) and <i>Odostomia plicata</i> (Montagu, 1803)) represent new BINs in BOLD database. This study contributes to new findings related to the high biodiversity of mollusks associated with widespread <i>C. officinalis</i> settlements along the southern coastal area of Istria. |
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| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-10T11:47:17Z |
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| spelling | doaj.art-7fc9e197326b4e39b0af1c52d66dd4bd2023-11-21T18:00:19ZengMDPI AGDiversity1424-28182021-04-0113519610.3390/d13050196DNA Barcoding of Marine Mollusks Associated with <i>Corallina officinalis</i> Turfs in Southern Istria (Adriatic Sea)Moira Buršić0Ljiljana Iveša1Andrej Jaklin2Milvana Arko Pijevac3Mladen Kučinić4Mauro Štifanić5Lucija Neal6Branka Bruvo Mađarić7Faculty of Natural Sciences, Juraj Dobrila University of Pula, Zagrebačka 30, 52100 Pula, CroatiaCenter for Marine Research, Ruđer Bošković Institute, G. Paliage 5, 52210 Rovinj, CroatiaCenter for Marine Research, Ruđer Bošković Institute, G. Paliage 5, 52210 Rovinj, CroatiaNatural History Museum Rijeka, Lorenzov Prolaz 1, 51000 Rijeka, CroatiaDepartment of Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, CroatiaFaculty of Natural Sciences, Juraj Dobrila University of Pula, Zagrebačka 30, 52100 Pula, CroatiaKaplan International College, Moulsecoomb Campus, University of Brighton, Watts Building, Lewes Rd., Brighton BN2 4GJ, UKMolecular Biology Division, Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, CroatiaPresence of mollusk assemblages was studied within red coralligenous algae <i>Corallina officinalis</i> L. along the southern Istrian coast. <i>C. officinalis</i> turfs can be considered a biodiversity reservoir, as they shelter numerous invertebrate species. The aim of this study was to identify mollusk species within these settlements using DNA barcoding as a method for detailed identification of mollusks. Nine locations and 18 localities with algal coverage range above 90% were chosen at four research areas. From 54 collected samples of <i>C. officinalis</i> turfs, a total of 46 mollusk species were identified. Molecular methods helped identify 16 gastropod, 14 bivalve and one polyplacophoran species. COI sequences for two bivalve species (<i>Musculus</i> cf. <i>costulatus</i> (Risso, 1826) and <i>Gregariella semigranata</i> (Reeve, 1858)) and seven gastropod species (<i>Megastomia winfriedi</i> Peñas & Rolán, 1999, <i>Eatonina</i> sp. Thiele, 1912, <i>Eatonina cossurae</i> (Calcara, 1841), <i>Crisilla</i> cf. <i>maculata</i> (Monterosato, 1869), <i>Alvania</i> cf. <i>carinata</i> (da Costa, 1778), <i>Vitreolina antiflexa</i> (Monterosato, 1884) and <i>Odostomia plicata</i> (Montagu, 1803)) represent new BINs in BOLD database. This study contributes to new findings related to the high biodiversity of mollusks associated with widespread <i>C. officinalis</i> settlements along the southern coastal area of Istria.https://www.mdpi.com/1424-2818/13/5/196mollusksgastropodsbivalves<i>Corallina officinalis</i>molecular identificationDNA barcoding |
| spellingShingle | Moira Buršić Ljiljana Iveša Andrej Jaklin Milvana Arko Pijevac Mladen Kučinić Mauro Štifanić Lucija Neal Branka Bruvo Mađarić DNA Barcoding of Marine Mollusks Associated with <i>Corallina officinalis</i> Turfs in Southern Istria (Adriatic Sea) Diversity mollusks gastropods bivalves <i>Corallina officinalis</i> molecular identification DNA barcoding |
| title | DNA Barcoding of Marine Mollusks Associated with <i>Corallina officinalis</i> Turfs in Southern Istria (Adriatic Sea) |
| title_full | DNA Barcoding of Marine Mollusks Associated with <i>Corallina officinalis</i> Turfs in Southern Istria (Adriatic Sea) |
| title_fullStr | DNA Barcoding of Marine Mollusks Associated with <i>Corallina officinalis</i> Turfs in Southern Istria (Adriatic Sea) |
| title_full_unstemmed | DNA Barcoding of Marine Mollusks Associated with <i>Corallina officinalis</i> Turfs in Southern Istria (Adriatic Sea) |
| title_short | DNA Barcoding of Marine Mollusks Associated with <i>Corallina officinalis</i> Turfs in Southern Istria (Adriatic Sea) |
| title_sort | dna barcoding of marine mollusks associated with i corallina officinalis i turfs in southern istria adriatic sea |
| topic | mollusks gastropods bivalves <i>Corallina officinalis</i> molecular identification DNA barcoding |
| url | https://www.mdpi.com/1424-2818/13/5/196 |
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