BCAS2 Regulates Delta-Notch Signaling Activity through Delta Pre-mRNA Splicing in Drosophila Wing Development.
Previously, we showed that BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing. In this study, we provide strong evidence that BCAS2 regulates the activity of Delta-Notch signaling via Delta pre-mRNA splicing. Depletion of dBCAS2 reduces Delta mRNA expression and leads to...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4475048?pdf=render |
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| author | Meng-Hsuan Chou Yi-Chen Hsieh Chu-Wei Huang Po-Han Chen Shih-Peng Chan Yeou-Ping Tsao Hsiu-Hsiang Lee June-Tai Wu Show-Li Chen |
| author_facet | Meng-Hsuan Chou Yi-Chen Hsieh Chu-Wei Huang Po-Han Chen Shih-Peng Chan Yeou-Ping Tsao Hsiu-Hsiang Lee June-Tai Wu Show-Li Chen |
| author_sort | Meng-Hsuan Chou |
| collection | DOAJ |
| description | Previously, we showed that BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing. In this study, we provide strong evidence that BCAS2 regulates the activity of Delta-Notch signaling via Delta pre-mRNA splicing. Depletion of dBCAS2 reduces Delta mRNA expression and leads to accumulation of Delta pre-mRNA, resulting in diminished transcriptions of Delta-Notch signaling target genes, such as cut and E(spl)m8. Furthermore, ectopic expression of human BCAS2 (hBCAS2) and Drosophila BCAS2 (dBCAS2) in a dBCAS2-deprived fly can rescue dBCAS2 depletion-induced wing damage to the normal phenotypes. These rescued phenotypes are correlated with the restoration of Delta pre-mRNA splicing, which affects Delta-Notch signaling activity. Additionally, overexpression of Delta can rescue the wing deformation by deprivation of dBCAS2; and the depletion of dBCAS2 can restore the aberrant eye associated with Delta-overexpressing retinas; providing supporting evidence for the regulation of Delta-Notch signaling by dBCAS2. Taken together, dBCAS2 participates in Delta pre-mRNA splicing that affects the regulation of Delta-Notch signaling in Drosophila wing development. |
| first_indexed | 2024-12-12T17:47:29Z |
| format | Article |
| id | doaj.art-7fcb61702acb458784f82e1c81a45f07 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T17:47:29Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7fcb61702acb458784f82e1c81a45f072022-12-22T00:16:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01106e013070610.1371/journal.pone.0130706BCAS2 Regulates Delta-Notch Signaling Activity through Delta Pre-mRNA Splicing in Drosophila Wing Development.Meng-Hsuan ChouYi-Chen HsiehChu-Wei HuangPo-Han ChenShih-Peng ChanYeou-Ping TsaoHsiu-Hsiang LeeJune-Tai WuShow-Li ChenPreviously, we showed that BCAS2 is essential for Drosophila viability and functions in pre-mRNA splicing. In this study, we provide strong evidence that BCAS2 regulates the activity of Delta-Notch signaling via Delta pre-mRNA splicing. Depletion of dBCAS2 reduces Delta mRNA expression and leads to accumulation of Delta pre-mRNA, resulting in diminished transcriptions of Delta-Notch signaling target genes, such as cut and E(spl)m8. Furthermore, ectopic expression of human BCAS2 (hBCAS2) and Drosophila BCAS2 (dBCAS2) in a dBCAS2-deprived fly can rescue dBCAS2 depletion-induced wing damage to the normal phenotypes. These rescued phenotypes are correlated with the restoration of Delta pre-mRNA splicing, which affects Delta-Notch signaling activity. Additionally, overexpression of Delta can rescue the wing deformation by deprivation of dBCAS2; and the depletion of dBCAS2 can restore the aberrant eye associated with Delta-overexpressing retinas; providing supporting evidence for the regulation of Delta-Notch signaling by dBCAS2. Taken together, dBCAS2 participates in Delta pre-mRNA splicing that affects the regulation of Delta-Notch signaling in Drosophila wing development.http://europepmc.org/articles/PMC4475048?pdf=render |
| spellingShingle | Meng-Hsuan Chou Yi-Chen Hsieh Chu-Wei Huang Po-Han Chen Shih-Peng Chan Yeou-Ping Tsao Hsiu-Hsiang Lee June-Tai Wu Show-Li Chen BCAS2 Regulates Delta-Notch Signaling Activity through Delta Pre-mRNA Splicing in Drosophila Wing Development. PLoS ONE |
| title | BCAS2 Regulates Delta-Notch Signaling Activity through Delta Pre-mRNA Splicing in Drosophila Wing Development. |
| title_full | BCAS2 Regulates Delta-Notch Signaling Activity through Delta Pre-mRNA Splicing in Drosophila Wing Development. |
| title_fullStr | BCAS2 Regulates Delta-Notch Signaling Activity through Delta Pre-mRNA Splicing in Drosophila Wing Development. |
| title_full_unstemmed | BCAS2 Regulates Delta-Notch Signaling Activity through Delta Pre-mRNA Splicing in Drosophila Wing Development. |
| title_short | BCAS2 Regulates Delta-Notch Signaling Activity through Delta Pre-mRNA Splicing in Drosophila Wing Development. |
| title_sort | bcas2 regulates delta notch signaling activity through delta pre mrna splicing in drosophila wing development |
| url | http://europepmc.org/articles/PMC4475048?pdf=render |
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