Effects of Nitrite Exposure on the Hematological Properties, Antioxidant and Stress Responses of Juvenile Hybrid Groupers, <i>Epinephelus lanceolatus</i> ♂ × <i>Epinephelus fuscoguttatus</i> ♀
Nitrite concentrations can reach high levels in indoor aquaculture systems, thus it is vital to determine the nitrite tolerance of aquaculture fish species. Here, juvenile hybrid groupers (<i>Epinephelus lanceolatus</i> ♂ × <i>Epinephelus fuscoguttatus</i> ♀, Family: Serranid...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-03-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/11/3/545 |
| _version_ | 1797473142957932544 |
|---|---|
| author | Jun-Hwan Kim Yue Jai Kang Kyung Mi Lee |
| author_facet | Jun-Hwan Kim Yue Jai Kang Kyung Mi Lee |
| author_sort | Jun-Hwan Kim |
| collection | DOAJ |
| description | Nitrite concentrations can reach high levels in indoor aquaculture systems, thus it is vital to determine the nitrite tolerance of aquaculture fish species. Here, juvenile hybrid groupers (<i>Epinephelus lanceolatus</i> ♂ × <i>Epinephelus fuscoguttatus</i> ♀, Family: Serranidae) were exposed to waterborne nitrite at 0, 10, 20, 40, and 80 mg NO<sub>2</sub><sup>−</sup>/L for 2 weeks. Nitrite exposure caused significant reductions in hematocrit and hemoglobin levels, significant increases in plasma calcium and plasma ALP levels, but had no significant effects on magnesium and total protein levels. Of the antioxidant responses investigated, SOD activity increased significantly in the liver and gills, but GST activity and GSH levels were significantly inhibited by nitrite exposure. Stress indicators, such as plasma cortisol and HSP 70 levels, were significantly stimulated by nitrite exposure. In brief, nitrite exposure over 20 mg NO<sub>2</sub><sup>−</sup>/L had toxic effects and affected the hematological properties, antioxidant responses, and stress indicators of juvenile hybrid groupers. |
| first_indexed | 2024-03-09T20:10:46Z |
| format | Article |
| id | doaj.art-7fcb6ba1ef274caf99700c87d192b65c |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T20:10:46Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-7fcb6ba1ef274caf99700c87d192b65c2023-11-24T00:18:34ZengMDPI AGAntioxidants2076-39212022-03-0111354510.3390/antiox11030545Effects of Nitrite Exposure on the Hematological Properties, Antioxidant and Stress Responses of Juvenile Hybrid Groupers, <i>Epinephelus lanceolatus</i> ♂ × <i>Epinephelus fuscoguttatus</i> ♀Jun-Hwan Kim0Yue Jai Kang1Kyung Mi Lee2Department of Aquatic Life and Medical Science, Sun Moon University, Asan 31460, KoreaDepartment of Aquatic Life and Medical Science, Sun Moon University, Asan 31460, KoreaNational Institute of Fisheries Science, West Sea Fisheries Research Institute, Incheon 22383, KoreaNitrite concentrations can reach high levels in indoor aquaculture systems, thus it is vital to determine the nitrite tolerance of aquaculture fish species. Here, juvenile hybrid groupers (<i>Epinephelus lanceolatus</i> ♂ × <i>Epinephelus fuscoguttatus</i> ♀, Family: Serranidae) were exposed to waterborne nitrite at 0, 10, 20, 40, and 80 mg NO<sub>2</sub><sup>−</sup>/L for 2 weeks. Nitrite exposure caused significant reductions in hematocrit and hemoglobin levels, significant increases in plasma calcium and plasma ALP levels, but had no significant effects on magnesium and total protein levels. Of the antioxidant responses investigated, SOD activity increased significantly in the liver and gills, but GST activity and GSH levels were significantly inhibited by nitrite exposure. Stress indicators, such as plasma cortisol and HSP 70 levels, were significantly stimulated by nitrite exposure. In brief, nitrite exposure over 20 mg NO<sub>2</sub><sup>−</sup>/L had toxic effects and affected the hematological properties, antioxidant responses, and stress indicators of juvenile hybrid groupers.https://www.mdpi.com/2076-3921/11/3/545hybrid groupernitrite exposurehematological propertiesantioxidant responsestress indicator |
| spellingShingle | Jun-Hwan Kim Yue Jai Kang Kyung Mi Lee Effects of Nitrite Exposure on the Hematological Properties, Antioxidant and Stress Responses of Juvenile Hybrid Groupers, <i>Epinephelus lanceolatus</i> ♂ × <i>Epinephelus fuscoguttatus</i> ♀ Antioxidants hybrid grouper nitrite exposure hematological properties antioxidant response stress indicator |
| title | Effects of Nitrite Exposure on the Hematological Properties, Antioxidant and Stress Responses of Juvenile Hybrid Groupers, <i>Epinephelus lanceolatus</i> ♂ × <i>Epinephelus fuscoguttatus</i> ♀ |
| title_full | Effects of Nitrite Exposure on the Hematological Properties, Antioxidant and Stress Responses of Juvenile Hybrid Groupers, <i>Epinephelus lanceolatus</i> ♂ × <i>Epinephelus fuscoguttatus</i> ♀ |
| title_fullStr | Effects of Nitrite Exposure on the Hematological Properties, Antioxidant and Stress Responses of Juvenile Hybrid Groupers, <i>Epinephelus lanceolatus</i> ♂ × <i>Epinephelus fuscoguttatus</i> ♀ |
| title_full_unstemmed | Effects of Nitrite Exposure on the Hematological Properties, Antioxidant and Stress Responses of Juvenile Hybrid Groupers, <i>Epinephelus lanceolatus</i> ♂ × <i>Epinephelus fuscoguttatus</i> ♀ |
| title_short | Effects of Nitrite Exposure on the Hematological Properties, Antioxidant and Stress Responses of Juvenile Hybrid Groupers, <i>Epinephelus lanceolatus</i> ♂ × <i>Epinephelus fuscoguttatus</i> ♀ |
| title_sort | effects of nitrite exposure on the hematological properties antioxidant and stress responses of juvenile hybrid groupers i epinephelus lanceolatus i ♂ i epinephelus fuscoguttatus i ♀ |
| topic | hybrid grouper nitrite exposure hematological properties antioxidant response stress indicator |
| url | https://www.mdpi.com/2076-3921/11/3/545 |
| work_keys_str_mv | AT junhwankim effectsofnitriteexposureonthehematologicalpropertiesantioxidantandstressresponsesofjuvenilehybridgroupersiepinepheluslanceolatusiiepinephelusfuscoguttatusi AT yuejaikang effectsofnitriteexposureonthehematologicalpropertiesantioxidantandstressresponsesofjuvenilehybridgroupersiepinepheluslanceolatusiiepinephelusfuscoguttatusi AT kyungmilee effectsofnitriteexposureonthehematologicalpropertiesantioxidantandstressresponsesofjuvenilehybridgroupersiepinepheluslanceolatusiiepinephelusfuscoguttatusi |