Effect of elevated CO<sub>2</sub> on the dynamics of particle-attached and free-living bacterioplankton communities in an Arctic fjord
In the frame of the European Project on Ocean Acidification (EPOCA), the response of an Arctic pelagic community (<3 mm) to a gradient of seawater <i>p</i>CO<sub>2</sub> was investigated. For this purpose 9 large-scale in situ mesocosms were deployed in Kongsfjorden, Svalb...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Copernicus Publications
2013-01-01
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| Series: | Biogeosciences |
| Online Access: | http://www.biogeosciences.net/10/181/2013/bg-10-181-2013.pdf |
| _version_ | 1818958168271618048 |
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| author | S. Romac U. Riebesell J. I. Nissimov L. Bittner J. Gilbert J. La Roche A.-S. Roy H. Schunck A. Wichels G. Gerdts J. Piontek M. Sperling A. Engel |
| author_facet | S. Romac U. Riebesell J. I. Nissimov L. Bittner J. Gilbert J. La Roche A.-S. Roy H. Schunck A. Wichels G. Gerdts J. Piontek M. Sperling A. Engel |
| author_sort | S. Romac |
| collection | DOAJ |
| description | In the frame of the European Project on Ocean Acidification (EPOCA), the response of an Arctic pelagic community (<3 mm) to a gradient of seawater <i>p</i>CO<sub>2</sub> was investigated. For this purpose 9 large-scale in situ mesocosms were deployed in Kongsfjorden, Svalbard (78°56.2´ N, 11°53.6´ E), in 2010. The present study investigates effects on the communities of particle-attached (PA; >3 μm) and free-living (FL; < 3 μm > 0.2 μm) bacteria by Automated Ribosomal Intergenic Spacer Analysis (ARISA) in 6 of the mesocosms, ranging from 185 to 1050 μatm initial <i>p</i>CO<sub>2</sub>, and the surrounding fjord. ARISA was able to resolve, on average, 27 bacterial band classes per sample and allowed for a detailed investigation of the explicit richness and diversity. Both, the PA and the FL bacterioplankton community exhibited a strong temporal development, which was driven mainly by temperature and phytoplankton development. In response to the breakdown of a picophytoplankton bloom, numbers of ARISA band classes in the PA community were reduced at low and medium CO<sub>2</sub> (~ 185–685 μatm) by about 25%, while they were more or less stable at high CO<sub>2</sub> (~ 820–1050 μatm). We hypothesise that enhanced viral lysis and enhanced availability of organic substrates at high CO<sub>2</sub> resulted in a more diverse PA bacterial community in the post-bloom phase. Despite lower cell numbers and extracellular enzyme activities in the post-bloom phase, bacterial protein production was enhanced in high CO<sub>2</sub> mesocosms, suggesting a positive effect of community richness on this function and on carbon cycling by bacteria. |
| first_indexed | 2024-12-20T11:21:27Z |
| format | Article |
| id | doaj.art-7fcbad0c1b124087ae2cf9c413c8cee1 |
| institution | Directory Open Access Journal |
| issn | 1726-4170 1726-4189 |
| language | English |
| last_indexed | 2024-12-20T11:21:27Z |
| publishDate | 2013-01-01 |
| publisher | Copernicus Publications |
| record_format | Article |
| series | Biogeosciences |
| spelling | doaj.art-7fcbad0c1b124087ae2cf9c413c8cee12022-12-21T19:42:30ZengCopernicus PublicationsBiogeosciences1726-41701726-41892013-01-0110118119110.5194/bg-10-181-2013Effect of elevated CO<sub>2</sub> on the dynamics of particle-attached and free-living bacterioplankton communities in an Arctic fjordS. RomacU. RiebesellJ. I. NissimovL. BittnerJ. GilbertJ. La RocheA.-S. RoyH. SchunckA. WichelsG. GerdtsJ. PiontekM. SperlingA. EngelIn the frame of the European Project on Ocean Acidification (EPOCA), the response of an Arctic pelagic community (<3 mm) to a gradient of seawater <i>p</i>CO<sub>2</sub> was investigated. For this purpose 9 large-scale in situ mesocosms were deployed in Kongsfjorden, Svalbard (78°56.2´ N, 11°53.6´ E), in 2010. The present study investigates effects on the communities of particle-attached (PA; >3 μm) and free-living (FL; < 3 μm > 0.2 μm) bacteria by Automated Ribosomal Intergenic Spacer Analysis (ARISA) in 6 of the mesocosms, ranging from 185 to 1050 μatm initial <i>p</i>CO<sub>2</sub>, and the surrounding fjord. ARISA was able to resolve, on average, 27 bacterial band classes per sample and allowed for a detailed investigation of the explicit richness and diversity. Both, the PA and the FL bacterioplankton community exhibited a strong temporal development, which was driven mainly by temperature and phytoplankton development. In response to the breakdown of a picophytoplankton bloom, numbers of ARISA band classes in the PA community were reduced at low and medium CO<sub>2</sub> (~ 185–685 μatm) by about 25%, while they were more or less stable at high CO<sub>2</sub> (~ 820–1050 μatm). We hypothesise that enhanced viral lysis and enhanced availability of organic substrates at high CO<sub>2</sub> resulted in a more diverse PA bacterial community in the post-bloom phase. Despite lower cell numbers and extracellular enzyme activities in the post-bloom phase, bacterial protein production was enhanced in high CO<sub>2</sub> mesocosms, suggesting a positive effect of community richness on this function and on carbon cycling by bacteria.http://www.biogeosciences.net/10/181/2013/bg-10-181-2013.pdf |
| spellingShingle | S. Romac U. Riebesell J. I. Nissimov L. Bittner J. Gilbert J. La Roche A.-S. Roy H. Schunck A. Wichels G. Gerdts J. Piontek M. Sperling A. Engel Effect of elevated CO<sub>2</sub> on the dynamics of particle-attached and free-living bacterioplankton communities in an Arctic fjord Biogeosciences |
| title | Effect of elevated CO<sub>2</sub> on the dynamics of particle-attached and free-living bacterioplankton communities in an Arctic fjord |
| title_full | Effect of elevated CO<sub>2</sub> on the dynamics of particle-attached and free-living bacterioplankton communities in an Arctic fjord |
| title_fullStr | Effect of elevated CO<sub>2</sub> on the dynamics of particle-attached and free-living bacterioplankton communities in an Arctic fjord |
| title_full_unstemmed | Effect of elevated CO<sub>2</sub> on the dynamics of particle-attached and free-living bacterioplankton communities in an Arctic fjord |
| title_short | Effect of elevated CO<sub>2</sub> on the dynamics of particle-attached and free-living bacterioplankton communities in an Arctic fjord |
| title_sort | effect of elevated co sub 2 sub on the dynamics of particle attached and free living bacterioplankton communities in an arctic fjord |
| url | http://www.biogeosciences.net/10/181/2013/bg-10-181-2013.pdf |
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