Marine Bioprospecting: Enzymes and Stress Proteins from the Sea Anemones <i>Anthopleura dowii</i> and <i>Lebrunia neglecta</i>
The bioprospecting of sea anemone tissues and secretions has revealed that they are natural libraries of polypeptides with diverse biological activities that can be utilized to develop of biotechnological tools with potential medical and industrial applications. This study conducted a proteomic anal...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/22/1/12 |
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| author | Santos Ramírez-Carreto Beatriz Miranda-Zaragoza Nuno Simões Ricardo González-Muñoz Claudia Rodríguez-Almazán |
| author_facet | Santos Ramírez-Carreto Beatriz Miranda-Zaragoza Nuno Simões Ricardo González-Muñoz Claudia Rodríguez-Almazán |
| author_sort | Santos Ramírez-Carreto |
| collection | DOAJ |
| description | The bioprospecting of sea anemone tissues and secretions has revealed that they are natural libraries of polypeptides with diverse biological activities that can be utilized to develop of biotechnological tools with potential medical and industrial applications. This study conducted a proteomic analysis of crude venom extracts from <i>Anthopleura dowii</i> Verrill, 1869, and <i>Lebrunia neglecta</i> Duchassaing & Michelotti, 1860. The obtained data allowed us to identify 201 polypeptides, of which 39% were present in both extracts. Among the obtained sequences, hydrolase-type enzymes, oxidoreductases, transferases, heat shock proteins, adhesion proteins, and protease inhibitors, among others, were identified. Interaction analysis and functional annotation indicated that these proteins are primarily involved in endoplasmic reticulum metabolic processes such as carbon metabolism and protein processing. In addition, several proteins related to oxidative stress were identified, including superoxide dismutase, peroxiredoxins, thioredoxin, and glutathione oxidase. Our results provide novel information on the polypeptide composition of the crude venom extract from sea anemones, which can be utilized to develop molecules for therapeutic tools and industrial applications. |
| first_indexed | 2024-03-08T10:43:26Z |
| format | Article |
| id | doaj.art-7fcd71fd250b47d69415faca303e69f8 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-08T10:43:26Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-7fcd71fd250b47d69415faca303e69f82024-01-26T17:25:51ZengMDPI AGMarine Drugs1660-33972023-12-012211210.3390/md22010012Marine Bioprospecting: Enzymes and Stress Proteins from the Sea Anemones <i>Anthopleura dowii</i> and <i>Lebrunia neglecta</i>Santos Ramírez-Carreto0Beatriz Miranda-Zaragoza1Nuno Simões2Ricardo González-Muñoz3Claudia Rodríguez-Almazán4Instituto Nacional de Salud Pública, Centro de Investigación Sobre Enfermedades Infecciosas, Av. Universidad #655, Santa María Ahuacatitlan, Cuernavaca C.P. 62100, MexicoDepartamento de Micro y Nanotecnologías, Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México, Cto. Exterior S/N, C.U., Coyoacán, Ciudad de México C.P. 04510, MexicoUnidad Multidisciplinaria de Docencia e Investigación en Sisal, Facultad de Ciencias, Universidad Nacional Autónoma de México, Puerto Abrigo s/n, Sisal C.P. 97356, MexicoInstituto de Investigaciones Marinas y Costeras, CONICET, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Dean Funes 3350, Mar del Plata C.P. 7600, ArgentinaDepartamento de Micro y Nanotecnologías, Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México, Cto. Exterior S/N, C.U., Coyoacán, Ciudad de México C.P. 04510, MexicoThe bioprospecting of sea anemone tissues and secretions has revealed that they are natural libraries of polypeptides with diverse biological activities that can be utilized to develop of biotechnological tools with potential medical and industrial applications. This study conducted a proteomic analysis of crude venom extracts from <i>Anthopleura dowii</i> Verrill, 1869, and <i>Lebrunia neglecta</i> Duchassaing & Michelotti, 1860. The obtained data allowed us to identify 201 polypeptides, of which 39% were present in both extracts. Among the obtained sequences, hydrolase-type enzymes, oxidoreductases, transferases, heat shock proteins, adhesion proteins, and protease inhibitors, among others, were identified. Interaction analysis and functional annotation indicated that these proteins are primarily involved in endoplasmic reticulum metabolic processes such as carbon metabolism and protein processing. In addition, several proteins related to oxidative stress were identified, including superoxide dismutase, peroxiredoxins, thioredoxin, and glutathione oxidase. Our results provide novel information on the polypeptide composition of the crude venom extract from sea anemones, which can be utilized to develop molecules for therapeutic tools and industrial applications.https://www.mdpi.com/1660-3397/22/1/12<i>Lebrunia neglecta</i><i>Anthopleura dowii</i>proteomecrude venom extractenzymessea anemone |
| spellingShingle | Santos Ramírez-Carreto Beatriz Miranda-Zaragoza Nuno Simões Ricardo González-Muñoz Claudia Rodríguez-Almazán Marine Bioprospecting: Enzymes and Stress Proteins from the Sea Anemones <i>Anthopleura dowii</i> and <i>Lebrunia neglecta</i> Marine Drugs <i>Lebrunia neglecta</i> <i>Anthopleura dowii</i> proteome crude venom extract enzymes sea anemone |
| title | Marine Bioprospecting: Enzymes and Stress Proteins from the Sea Anemones <i>Anthopleura dowii</i> and <i>Lebrunia neglecta</i> |
| title_full | Marine Bioprospecting: Enzymes and Stress Proteins from the Sea Anemones <i>Anthopleura dowii</i> and <i>Lebrunia neglecta</i> |
| title_fullStr | Marine Bioprospecting: Enzymes and Stress Proteins from the Sea Anemones <i>Anthopleura dowii</i> and <i>Lebrunia neglecta</i> |
| title_full_unstemmed | Marine Bioprospecting: Enzymes and Stress Proteins from the Sea Anemones <i>Anthopleura dowii</i> and <i>Lebrunia neglecta</i> |
| title_short | Marine Bioprospecting: Enzymes and Stress Proteins from the Sea Anemones <i>Anthopleura dowii</i> and <i>Lebrunia neglecta</i> |
| title_sort | marine bioprospecting enzymes and stress proteins from the sea anemones i anthopleura dowii i and i lebrunia neglecta i |
| topic | <i>Lebrunia neglecta</i> <i>Anthopleura dowii</i> proteome crude venom extract enzymes sea anemone |
| url | https://www.mdpi.com/1660-3397/22/1/12 |
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