Systemic changes in cell size throughout the body of Drosophila melanogaster associated with mutations in molecular cell cycle regulators
Abstract Along with different life strategies, organisms have evolved dramatic cellular composition differences. Understanding the molecular basis and fitness effects of these differences is key to elucidating the fundamental characteristics of life. TOR/insulin pathways are key regulators of cell s...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-34674-y |
| _version_ | 1797827579946729472 |
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| author | Valeriya Privalova Anna Maria Labecka Ewa Szlachcic Anna Sikorska Marcin Czarnoleski |
| author_facet | Valeriya Privalova Anna Maria Labecka Ewa Szlachcic Anna Sikorska Marcin Czarnoleski |
| author_sort | Valeriya Privalova |
| collection | DOAJ |
| description | Abstract Along with different life strategies, organisms have evolved dramatic cellular composition differences. Understanding the molecular basis and fitness effects of these differences is key to elucidating the fundamental characteristics of life. TOR/insulin pathways are key regulators of cell size, but whether their activity determines cell size in a systemic or tissue-specific manner awaits exploration. To that end, we measured cells in four tissues in genetically modified Drosophila melanogaster (rictor Δ2 and Mnt 1 ) and corresponding controls. While rictor Δ2 flies lacked the Rictor protein in TOR complex 2, downregulating the functions of this element in TOR/insulin pathways, Mnt 1 flies lacked the transcriptional regulator protein Mnt, weakening the suppression of downstream signalling from TOR/insulin pathways. rictor Δ2 flies had smaller epidermal (leg and wing) and ommatidial cells and Mnt 1 flies had larger cells in these tissues than the controls. Females had consistently larger cells than males in the three tissue types. In contrast, dorsal longitudinal flight muscle cells (measured only in males) were not altered by mutations. We suggest that mutations in cell cycle control pathways drive the evolution of systemic changes in cell size throughout the body, but additional mechanisms shape the cellular composition of some tissues independent of these mutations. |
| first_indexed | 2024-04-09T12:50:35Z |
| format | Article |
| id | doaj.art-7fcec753147d4ca3a82c769a46b574d6 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T12:50:35Z |
| publishDate | 2023-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7fcec753147d4ca3a82c769a46b574d62023-05-14T11:13:51ZengNature PortfolioScientific Reports2045-23222023-05-0113111110.1038/s41598-023-34674-ySystemic changes in cell size throughout the body of Drosophila melanogaster associated with mutations in molecular cell cycle regulatorsValeriya Privalova0Anna Maria Labecka1Ewa Szlachcic2Anna Sikorska3Marcin Czarnoleski4Life History Evolution Group, Institute of Environmental Sciences, Faculty of Biology, Jagiellonian UniversityLife History Evolution Group, Institute of Environmental Sciences, Faculty of Biology, Jagiellonian UniversityLife History Evolution Group, Institute of Environmental Sciences, Faculty of Biology, Jagiellonian UniversityLife History Evolution Group, Institute of Environmental Sciences, Faculty of Biology, Jagiellonian UniversityLife History Evolution Group, Institute of Environmental Sciences, Faculty of Biology, Jagiellonian UniversityAbstract Along with different life strategies, organisms have evolved dramatic cellular composition differences. Understanding the molecular basis and fitness effects of these differences is key to elucidating the fundamental characteristics of life. TOR/insulin pathways are key regulators of cell size, but whether their activity determines cell size in a systemic or tissue-specific manner awaits exploration. To that end, we measured cells in four tissues in genetically modified Drosophila melanogaster (rictor Δ2 and Mnt 1 ) and corresponding controls. While rictor Δ2 flies lacked the Rictor protein in TOR complex 2, downregulating the functions of this element in TOR/insulin pathways, Mnt 1 flies lacked the transcriptional regulator protein Mnt, weakening the suppression of downstream signalling from TOR/insulin pathways. rictor Δ2 flies had smaller epidermal (leg and wing) and ommatidial cells and Mnt 1 flies had larger cells in these tissues than the controls. Females had consistently larger cells than males in the three tissue types. In contrast, dorsal longitudinal flight muscle cells (measured only in males) were not altered by mutations. We suggest that mutations in cell cycle control pathways drive the evolution of systemic changes in cell size throughout the body, but additional mechanisms shape the cellular composition of some tissues independent of these mutations.https://doi.org/10.1038/s41598-023-34674-y |
| spellingShingle | Valeriya Privalova Anna Maria Labecka Ewa Szlachcic Anna Sikorska Marcin Czarnoleski Systemic changes in cell size throughout the body of Drosophila melanogaster associated with mutations in molecular cell cycle regulators Scientific Reports |
| title | Systemic changes in cell size throughout the body of Drosophila melanogaster associated with mutations in molecular cell cycle regulators |
| title_full | Systemic changes in cell size throughout the body of Drosophila melanogaster associated with mutations in molecular cell cycle regulators |
| title_fullStr | Systemic changes in cell size throughout the body of Drosophila melanogaster associated with mutations in molecular cell cycle regulators |
| title_full_unstemmed | Systemic changes in cell size throughout the body of Drosophila melanogaster associated with mutations in molecular cell cycle regulators |
| title_short | Systemic changes in cell size throughout the body of Drosophila melanogaster associated with mutations in molecular cell cycle regulators |
| title_sort | systemic changes in cell size throughout the body of drosophila melanogaster associated with mutations in molecular cell cycle regulators |
| url | https://doi.org/10.1038/s41598-023-34674-y |
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