Metabolomics of Cerebrospinal Fluid from Healthy Subjects Reveal Metabolites Associated with Ageing
To increase our understanding of age-related diseases affecting the central nervous system (CNS) it is important to understand the molecular processes of biological ageing. Metabolomics of cerebrospinal fluid (CSF) is a promising methodology to increase our understanding of naturally occurring proce...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/11/2/126 |
| _version_ | 1797395512265015296 |
|---|---|
| author | Henrik Carlsson Niclas Rollborn Stephanie Herman Eva Freyhult Anders Svenningsson Joachim Burman Kim Kultima |
| author_facet | Henrik Carlsson Niclas Rollborn Stephanie Herman Eva Freyhult Anders Svenningsson Joachim Burman Kim Kultima |
| author_sort | Henrik Carlsson |
| collection | DOAJ |
| description | To increase our understanding of age-related diseases affecting the central nervous system (CNS) it is important to understand the molecular processes of biological ageing. Metabolomics of cerebrospinal fluid (CSF) is a promising methodology to increase our understanding of naturally occurring processes of ageing of the brain and CNS that could be reflected in CSF. In the present study the CSF metabolomes of healthy subjects aged 30–74 years (<i>n</i> = 23) were studied using liquid chromatography high-resolution mass spectrometry (LC-HRMS), and investigated in relation to age. Ten metabolites were identified with high confidence as significantly associated with ageing, eight with increasing levels with ageing: isoleucine, acetylcarnitine, pipecolate, methionine, glutarylcarnitine, 5-hydroxytryptophan, ketoleucine, and hippurate; and two decreasing with ageing: methylthioadenosine and 3-methyladenine. To our knowledge, this is the first time the CSF metabolomes of healthy subjects are assessed in relation to ageing. The present study contributes to the field of ageing metabolomics by presenting a number of metabolites present in CSF with potential relevance for ageing and the results motivate further studies. |
| first_indexed | 2024-03-09T00:36:35Z |
| format | Article |
| id | doaj.art-7fd0f9c2027d4ce88cbee67673d42e6a |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T00:36:35Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-7fd0f9c2027d4ce88cbee67673d42e6a2023-12-11T18:08:11ZengMDPI AGMetabolites2218-19892021-02-0111212610.3390/metabo11020126Metabolomics of Cerebrospinal Fluid from Healthy Subjects Reveal Metabolites Associated with AgeingHenrik Carlsson0Niclas Rollborn1Stephanie Herman2Eva Freyhult3Anders Svenningsson4Joachim Burman5Kim Kultima6Department of Medical Sciences, Clinical Chemistry, Uppsala University, 751 85 Uppsala, SwedenDepartment of Medical Sciences, Clinical Chemistry, Uppsala University, 751 85 Uppsala, SwedenDepartment of Medical Sciences, Clinical Chemistry, Uppsala University, 751 85 Uppsala, SwedenDepartment of Medical Sciences, Clinical Chemistry, Uppsala University, 751 85 Uppsala, SwedenDepartment of Clinical Sciences, Karolinska Institutet, Danderyd Hospital, 182 88 Stockholm, SwedenDepartment of Neuroscience, Uppsala University, 751 85 Uppsala, SwedenDepartment of Medical Sciences, Clinical Chemistry, Uppsala University, 751 85 Uppsala, SwedenTo increase our understanding of age-related diseases affecting the central nervous system (CNS) it is important to understand the molecular processes of biological ageing. Metabolomics of cerebrospinal fluid (CSF) is a promising methodology to increase our understanding of naturally occurring processes of ageing of the brain and CNS that could be reflected in CSF. In the present study the CSF metabolomes of healthy subjects aged 30–74 years (<i>n</i> = 23) were studied using liquid chromatography high-resolution mass spectrometry (LC-HRMS), and investigated in relation to age. Ten metabolites were identified with high confidence as significantly associated with ageing, eight with increasing levels with ageing: isoleucine, acetylcarnitine, pipecolate, methionine, glutarylcarnitine, 5-hydroxytryptophan, ketoleucine, and hippurate; and two decreasing with ageing: methylthioadenosine and 3-methyladenine. To our knowledge, this is the first time the CSF metabolomes of healthy subjects are assessed in relation to ageing. The present study contributes to the field of ageing metabolomics by presenting a number of metabolites present in CSF with potential relevance for ageing and the results motivate further studies.https://www.mdpi.com/2218-1989/11/2/126metabolomicscerebrospinal fluidageinghigh-resolution mass spectrometry |
| spellingShingle | Henrik Carlsson Niclas Rollborn Stephanie Herman Eva Freyhult Anders Svenningsson Joachim Burman Kim Kultima Metabolomics of Cerebrospinal Fluid from Healthy Subjects Reveal Metabolites Associated with Ageing Metabolites metabolomics cerebrospinal fluid ageing high-resolution mass spectrometry |
| title | Metabolomics of Cerebrospinal Fluid from Healthy Subjects Reveal Metabolites Associated with Ageing |
| title_full | Metabolomics of Cerebrospinal Fluid from Healthy Subjects Reveal Metabolites Associated with Ageing |
| title_fullStr | Metabolomics of Cerebrospinal Fluid from Healthy Subjects Reveal Metabolites Associated with Ageing |
| title_full_unstemmed | Metabolomics of Cerebrospinal Fluid from Healthy Subjects Reveal Metabolites Associated with Ageing |
| title_short | Metabolomics of Cerebrospinal Fluid from Healthy Subjects Reveal Metabolites Associated with Ageing |
| title_sort | metabolomics of cerebrospinal fluid from healthy subjects reveal metabolites associated with ageing |
| topic | metabolomics cerebrospinal fluid ageing high-resolution mass spectrometry |
| url | https://www.mdpi.com/2218-1989/11/2/126 |
| work_keys_str_mv | AT henrikcarlsson metabolomicsofcerebrospinalfluidfromhealthysubjectsrevealmetabolitesassociatedwithageing AT niclasrollborn metabolomicsofcerebrospinalfluidfromhealthysubjectsrevealmetabolitesassociatedwithageing AT stephanieherman metabolomicsofcerebrospinalfluidfromhealthysubjectsrevealmetabolitesassociatedwithageing AT evafreyhult metabolomicsofcerebrospinalfluidfromhealthysubjectsrevealmetabolitesassociatedwithageing AT anderssvenningsson metabolomicsofcerebrospinalfluidfromhealthysubjectsrevealmetabolitesassociatedwithageing AT joachimburman metabolomicsofcerebrospinalfluidfromhealthysubjectsrevealmetabolitesassociatedwithageing AT kimkultima metabolomicsofcerebrospinalfluidfromhealthysubjectsrevealmetabolitesassociatedwithageing |