Serum IGF-1, IGFBP-3 and their ratio: Potential biochemical growth maturity indicators
Abstract Background Determination of skeletal maturation and remaining growth potential is an essential part of treatment planning in orthodontics. The aim of our study was to determine the relationship between IGF-1 levels, IGFBP-3 levels with CVM staging to track the pre pubertal and pubertal grow...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-05-01
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| Series: | Progress in Orthodontics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40510-017-0165-1 |
| _version_ | 1818951034231324672 |
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| author | Nimisha Jain Tulika Tripathi S. K. Gupta Priyank Rai Anup Kanase Shilpa Kalra |
| author_facet | Nimisha Jain Tulika Tripathi S. K. Gupta Priyank Rai Anup Kanase Shilpa Kalra |
| author_sort | Nimisha Jain |
| collection | DOAJ |
| description | Abstract Background Determination of skeletal maturation and remaining growth potential is an essential part of treatment planning in orthodontics. The aim of our study was to determine the relationship between IGF-1 levels, IGFBP-3 levels with CVM staging to track the pre pubertal and pubertal growth spurts in female patients in North Indian population. Methods This cross-sectional study was conducted on ninety female subjects in the age group of 8-20 years. Blood samples were collected and centrifuged and serum samples were then analysed by Human IGF-1 and IGFBP-3 enzyme-linked immunosorbent assay kits, specific for IGF-1 and IGFBP-3, respectively. CVM staging on lateral cephalometric radiograph was determined for all patients. Analysis of variance test followed by a post hoc test was used to compare mean IGF-1 and IGFBP-3 corresponding to six stages of cervical vertebrae maturation stages. Linear Pearson’s correlations were performed to determine the trends of IGF-1, IGFBP-3, and its ratio relating to CVM stage. The kappa statistic was used to measure inter and intra examiner reliability. P value <0.05 was considered as statistically significant. Results Mean serum IGF-1 levels were found to be highest (403.3 ± 12.3 ng/ml) at CVMI3 stage of CVMI. The post-hoc test revealed a significant difference in IGF-1 levels between all stages of CVMI, thereby indicating a specific range of IGF-1 levels for a specific skeletal stage. Mean serum IGFBP-3 levels were found to be highest (5186.8 ± 1384.2 ng/ml) at CVMI4 stage of CVMI. The mean serum IGFBP-3 levels at CVMI4 were found to be significantly higher than the levels at all other CVMI stages except CVMI3 stage. Conclusions IGF-1 and IGFBP-3 can serve as a potential biochemical indicator for assessment of skeletal maturity. |
| first_indexed | 2024-12-20T09:28:04Z |
| format | Article |
| id | doaj.art-7fd29f2e80cc46f798483dbd01248cdb |
| institution | Directory Open Access Journal |
| issn | 2196-1042 |
| language | English |
| last_indexed | 2024-12-20T09:28:04Z |
| publishDate | 2017-05-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Progress in Orthodontics |
| spelling | doaj.art-7fd29f2e80cc46f798483dbd01248cdb2022-12-21T19:45:09ZengSpringerOpenProgress in Orthodontics2196-10422017-05-011811810.1186/s40510-017-0165-1Serum IGF-1, IGFBP-3 and their ratio: Potential biochemical growth maturity indicatorsNimisha JainTulika Tripathi0S. K. Gupta1Priyank Rai2Anup Kanase3Shilpa Kalra4Department of Orthodontics and Dentofacial Orthopaedics, Maulana Azad Institute of Dental SciencesDepartment of Biochemistry, Maulana Azad Medical CollegeDepartment of Orthodontics and Dentofacial Orthopaedics, Maulana Azad Institute of Dental SciencesDepartment of Orthodontics and Dentofacial Orthopaedics, Maulana Azad Institute of Dental SciencesDepartment of Orthodontics and Dentofacial Orthopaedics, Maulana Azad Institute of Dental SciencesAbstract Background Determination of skeletal maturation and remaining growth potential is an essential part of treatment planning in orthodontics. The aim of our study was to determine the relationship between IGF-1 levels, IGFBP-3 levels with CVM staging to track the pre pubertal and pubertal growth spurts in female patients in North Indian population. Methods This cross-sectional study was conducted on ninety female subjects in the age group of 8-20 years. Blood samples were collected and centrifuged and serum samples were then analysed by Human IGF-1 and IGFBP-3 enzyme-linked immunosorbent assay kits, specific for IGF-1 and IGFBP-3, respectively. CVM staging on lateral cephalometric radiograph was determined for all patients. Analysis of variance test followed by a post hoc test was used to compare mean IGF-1 and IGFBP-3 corresponding to six stages of cervical vertebrae maturation stages. Linear Pearson’s correlations were performed to determine the trends of IGF-1, IGFBP-3, and its ratio relating to CVM stage. The kappa statistic was used to measure inter and intra examiner reliability. P value <0.05 was considered as statistically significant. Results Mean serum IGF-1 levels were found to be highest (403.3 ± 12.3 ng/ml) at CVMI3 stage of CVMI. The post-hoc test revealed a significant difference in IGF-1 levels between all stages of CVMI, thereby indicating a specific range of IGF-1 levels for a specific skeletal stage. Mean serum IGFBP-3 levels were found to be highest (5186.8 ± 1384.2 ng/ml) at CVMI4 stage of CVMI. The mean serum IGFBP-3 levels at CVMI4 were found to be significantly higher than the levels at all other CVMI stages except CVMI3 stage. Conclusions IGF-1 and IGFBP-3 can serve as a potential biochemical indicator for assessment of skeletal maturity.http://link.springer.com/article/10.1186/s40510-017-0165-1IGF-1IGFBP-3Biochemical growth marker |
| spellingShingle | Nimisha Jain Tulika Tripathi S. K. Gupta Priyank Rai Anup Kanase Shilpa Kalra Serum IGF-1, IGFBP-3 and their ratio: Potential biochemical growth maturity indicators Progress in Orthodontics IGF-1 IGFBP-3 Biochemical growth marker |
| title | Serum IGF-1, IGFBP-3 and their ratio: Potential biochemical growth maturity indicators |
| title_full | Serum IGF-1, IGFBP-3 and their ratio: Potential biochemical growth maturity indicators |
| title_fullStr | Serum IGF-1, IGFBP-3 and their ratio: Potential biochemical growth maturity indicators |
| title_full_unstemmed | Serum IGF-1, IGFBP-3 and their ratio: Potential biochemical growth maturity indicators |
| title_short | Serum IGF-1, IGFBP-3 and their ratio: Potential biochemical growth maturity indicators |
| title_sort | serum igf 1 igfbp 3 and their ratio potential biochemical growth maturity indicators |
| topic | IGF-1 IGFBP-3 Biochemical growth marker |
| url | http://link.springer.com/article/10.1186/s40510-017-0165-1 |
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