Novel application of anti‐human Fc nanobody for screening high‐producing CHO cells for monoclonal antibody
Abstract Animal‐derived anti‐IgG secondary antibodies are currently employed to stain and screen of human monoclonal antibody(mAb)‐producing cells, but using animal‐derived antibodies may raise the concerns of high cost, complicated operations and biological safety issues in biopharmaceutical manufa...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2022-10-01
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| Series: | Engineering in Life Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/elsc.202200028 |
| _version_ | 1811195144339193856 |
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| author | Di Zhu Zheng Wang Yunxia Xu Jing Lin Mei Qiu Jianghai Liu Xinlei Li |
| author_facet | Di Zhu Zheng Wang Yunxia Xu Jing Lin Mei Qiu Jianghai Liu Xinlei Li |
| author_sort | Di Zhu |
| collection | DOAJ |
| description | Abstract Animal‐derived anti‐IgG secondary antibodies are currently employed to stain and screen of human monoclonal antibody(mAb)‐producing cells, but using animal‐derived antibodies may raise the concerns of high cost, complicated operations and biological safety issues in biopharmaceutical manufacturing. Nanobodies(VHHs) are attractive forms of antibodies for their straightforward engineering and expression in both eukaryotic and prokaryotic systems. Using phage‐displayed immune llama VHH library, we identified new anti‐Fc VHHs that could bind to human Fc with high affinity. In GFP fusion format, the anti‐Fc VHH‐GFP generated dramatically stronger FACS signals than AF488 conjugated anti‐IgG antibodies when used for staining mAb‐producing CHO cells. Furthermore, preparative sorting of CHO cells based on anti‐Fc VHH‐GFP staining resulted in the enrichment of cell lines capable of synthesizing mAb at high productivity. This safe and cost‐efficient anti‐Fc VHH‐GFP may optimize the process of generating highly productive cell lines for therapeutic mAb production compared to conventional animal‐derived fluorescent antibodies. |
| first_indexed | 2024-04-12T00:38:33Z |
| format | Article |
| id | doaj.art-7fd2c599aa0740e5a361142c03a5b841 |
| institution | Directory Open Access Journal |
| issn | 1618-0240 1618-2863 |
| language | English |
| last_indexed | 2024-04-12T00:38:33Z |
| publishDate | 2022-10-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Engineering in Life Sciences |
| spelling | doaj.art-7fd2c599aa0740e5a361142c03a5b8412022-12-22T03:55:05ZengWiley-VCHEngineering in Life Sciences1618-02401618-28632022-10-01221060861810.1002/elsc.202200028Novel application of anti‐human Fc nanobody for screening high‐producing CHO cells for monoclonal antibodyDi Zhu0Zheng Wang1Yunxia Xu2Jing Lin3Mei Qiu4Jianghai Liu5Xinlei Li6Chengdu Medical College Sichuan Province ChinaShanghai Bao Pharmaceuticals Co. Ltd. Shanghai ChinaShanghai Bao Pharmaceuticals Co. Ltd. Shanghai ChinaABLINK Biotech Co. Ltd. Chengdu ChinaABLINK Biotech Co. Ltd. Chengdu ChinaChengdu Medical College Sichuan Province ChinaChengdu Medical College Sichuan Province ChinaAbstract Animal‐derived anti‐IgG secondary antibodies are currently employed to stain and screen of human monoclonal antibody(mAb)‐producing cells, but using animal‐derived antibodies may raise the concerns of high cost, complicated operations and biological safety issues in biopharmaceutical manufacturing. Nanobodies(VHHs) are attractive forms of antibodies for their straightforward engineering and expression in both eukaryotic and prokaryotic systems. Using phage‐displayed immune llama VHH library, we identified new anti‐Fc VHHs that could bind to human Fc with high affinity. In GFP fusion format, the anti‐Fc VHH‐GFP generated dramatically stronger FACS signals than AF488 conjugated anti‐IgG antibodies when used for staining mAb‐producing CHO cells. Furthermore, preparative sorting of CHO cells based on anti‐Fc VHH‐GFP staining resulted in the enrichment of cell lines capable of synthesizing mAb at high productivity. This safe and cost‐efficient anti‐Fc VHH‐GFP may optimize the process of generating highly productive cell lines for therapeutic mAb production compared to conventional animal‐derived fluorescent antibodies.https://doi.org/10.1002/elsc.202200028Chinese hamster ovary cellGFPmonoclonal antibodynanobodyphage display |
| spellingShingle | Di Zhu Zheng Wang Yunxia Xu Jing Lin Mei Qiu Jianghai Liu Xinlei Li Novel application of anti‐human Fc nanobody for screening high‐producing CHO cells for monoclonal antibody Engineering in Life Sciences Chinese hamster ovary cell GFP monoclonal antibody nanobody phage display |
| title | Novel application of anti‐human Fc nanobody for screening high‐producing CHO cells for monoclonal antibody |
| title_full | Novel application of anti‐human Fc nanobody for screening high‐producing CHO cells for monoclonal antibody |
| title_fullStr | Novel application of anti‐human Fc nanobody for screening high‐producing CHO cells for monoclonal antibody |
| title_full_unstemmed | Novel application of anti‐human Fc nanobody for screening high‐producing CHO cells for monoclonal antibody |
| title_short | Novel application of anti‐human Fc nanobody for screening high‐producing CHO cells for monoclonal antibody |
| title_sort | novel application of anti human fc nanobody for screening high producing cho cells for monoclonal antibody |
| topic | Chinese hamster ovary cell GFP monoclonal antibody nanobody phage display |
| url | https://doi.org/10.1002/elsc.202200028 |
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