Identification and Breeding with Genome Shuffling Strain CLYB1 Producing γ-Aminobutyric Acid
In order to improve the yield of γ-aminobutyric acid (GABA), strain CLYB1 was bred with ultraviolet mutagenesis and genome shuffling to produce high yield of GABA. After breeding，the hemolysis experiments and antibiotic sensitivity test were carried out on the recombinant strain. The strain CLYB1 wa...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-10-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023040233 |
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| author | Yanping CHI Xinyu MIAO Jinghui WANG Ying SU Honghong NIU Mubai SUN Da LI Mei HUA Yonggang DAI |
| author_facet | Yanping CHI Xinyu MIAO Jinghui WANG Ying SU Honghong NIU Mubai SUN Da LI Mei HUA Yonggang DAI |
| author_sort | Yanping CHI |
| collection | DOAJ |
| description | In order to improve the yield of γ-aminobutyric acid (GABA), strain CLYB1 was bred with ultraviolet mutagenesis and genome shuffling to produce high yield of GABA. After breeding，the hemolysis experiments and antibiotic sensitivity test were carried out on the recombinant strain. The strain CLYB1 was Bacillus velezensis, and the yield of GABA was 3.95 g/L. The GABA yield of CLYB1-Y breeding by ultraviolet mutagenesis from CLYB1 was 10.26 g/L, which was 160% higher than that of CLYB1. The GABA yield of CLYB1-YC breeding by genome shuffling from CLYB1-Y was 20.19 g/L, which was 411% higher than that of CLYB1. The hemolysis experiments and antibiotic sensitivity tests showed that CLYB1-YC had no hemolytic activity and was sensitive to 10 common antibiotics (penicillin, ampicillin, ceftriaxone, gentamycin, tetracycline, erythromycin, ciprofloxacin, lincomycin, chloramphenicol and puromyn). The Bacillus velezensis CLYB1-YC breeding by genome shuffling producing high γ-aminobutyric acid has a good application prospect. |
| first_indexed | 2024-03-11T18:51:45Z |
| format | Article |
| id | doaj.art-7fd450eb9c454c1a8dbdfca529de323d |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-11T18:51:45Z |
| publishDate | 2023-10-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-7fd450eb9c454c1a8dbdfca529de323d2023-10-11T09:18:20ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-10-01441916717310.13386/j.issn1002-0306.20230402332023040233-19Identification and Breeding with Genome Shuffling Strain CLYB1 Producing γ-Aminobutyric AcidYanping CHI0Xinyu MIAO1Jinghui WANG2Ying SU3Honghong NIU4Mubai SUN5Da LI6Mei HUA7Yonggang DAI8Institute of Argo-food Technology, Jilin Academy of Agricultural Science, Changchun 130033, ChinaInstitute of Argo-food Technology, Jilin Academy of Agricultural Science, Changchun 130033, ChinaInstitute of Argo-food Technology, Jilin Academy of Agricultural Science, Changchun 130033, ChinaInstitute of Argo-food Technology, Jilin Academy of Agricultural Science, Changchun 130033, ChinaInstitute of Argo-food Technology, Jilin Academy of Agricultural Science, Changchun 130033, ChinaInstitute of Argo-food Technology, Jilin Academy of Agricultural Science, Changchun 130033, ChinaInstitute of Argo-food Technology, Jilin Academy of Agricultural Science, Changchun 130033, ChinaInstitute of Argo-food Technology, Jilin Academy of Agricultural Science, Changchun 130033, ChinaInstitute of Argo-food Technology, Jilin Academy of Agricultural Science, Changchun 130033, ChinaIn order to improve the yield of γ-aminobutyric acid (GABA), strain CLYB1 was bred with ultraviolet mutagenesis and genome shuffling to produce high yield of GABA. After breeding，the hemolysis experiments and antibiotic sensitivity test were carried out on the recombinant strain. The strain CLYB1 was Bacillus velezensis, and the yield of GABA was 3.95 g/L. The GABA yield of CLYB1-Y breeding by ultraviolet mutagenesis from CLYB1 was 10.26 g/L, which was 160% higher than that of CLYB1. The GABA yield of CLYB1-YC breeding by genome shuffling from CLYB1-Y was 20.19 g/L, which was 411% higher than that of CLYB1. The hemolysis experiments and antibiotic sensitivity tests showed that CLYB1-YC had no hemolytic activity and was sensitive to 10 common antibiotics (penicillin, ampicillin, ceftriaxone, gentamycin, tetracycline, erythromycin, ciprofloxacin, lincomycin, chloramphenicol and puromyn). The Bacillus velezensis CLYB1-YC breeding by genome shuffling producing high γ-aminobutyric acid has a good application prospect.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023040233bacillus velezensis clyb1γ-aminobutyric acididentificationmutagenesisgenome shuffling |
| spellingShingle | Yanping CHI Xinyu MIAO Jinghui WANG Ying SU Honghong NIU Mubai SUN Da LI Mei HUA Yonggang DAI Identification and Breeding with Genome Shuffling Strain CLYB1 Producing γ-Aminobutyric Acid Shipin gongye ke-ji bacillus velezensis clyb1 γ-aminobutyric acid identification mutagenesis genome shuffling |
| title | Identification and Breeding with Genome Shuffling Strain CLYB1 Producing γ-Aminobutyric Acid |
| title_full | Identification and Breeding with Genome Shuffling Strain CLYB1 Producing γ-Aminobutyric Acid |
| title_fullStr | Identification and Breeding with Genome Shuffling Strain CLYB1 Producing γ-Aminobutyric Acid |
| title_full_unstemmed | Identification and Breeding with Genome Shuffling Strain CLYB1 Producing γ-Aminobutyric Acid |
| title_short | Identification and Breeding with Genome Shuffling Strain CLYB1 Producing γ-Aminobutyric Acid |
| title_sort | identification and breeding with genome shuffling strain clyb1 producing γ aminobutyric acid |
| topic | bacillus velezensis clyb1 γ-aminobutyric acid identification mutagenesis genome shuffling |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2023040233 |
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