Complement activation products in tears of dry eye and meibomian gland dysfunction
Abstract Sixty-seven patients (38 woman; median age, 69 years) were enrolled to assess complement activation products (CAPs) in tear fluid with/without dry eye (DE) and with/without meibomian gland dysfunction (MGD). Patients were divided into four groups based on the presence/absence of DE and MGD:...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-46634-7 |
| _version_ | 1797363377815683072 |
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| author | Hiroki Maehara Koki Norikawa Keiichiro Tanaka Yutaka Kato Akihito Kasai Ryo Mukai Tomoko Omori Takeshi Machida Hideharu Sekine Tetsuju Sekiryu |
| author_facet | Hiroki Maehara Koki Norikawa Keiichiro Tanaka Yutaka Kato Akihito Kasai Ryo Mukai Tomoko Omori Takeshi Machida Hideharu Sekine Tetsuju Sekiryu |
| author_sort | Hiroki Maehara |
| collection | DOAJ |
| description | Abstract Sixty-seven patients (38 woman; median age, 69 years) were enrolled to assess complement activation products (CAPs) in tear fluid with/without dry eye (DE) and with/without meibomian gland dysfunction (MGD). Patients were divided into four groups based on the presence/absence of DE and MGD: group DM had both DE and MGD, group DN had DE without MGD, group NM had MGD without DE, and group NN had neither DE nor MGD. The levels of C3a and C5a in the collected tears were analyzed using a cytometric bead array. The C3a concentrations in the DM, DN, NM, and NN groups were 2326 pg/ml, 1411 pg/ml, 1821 pg/ml, and 978 pg/ml, respectively. The C5a concentrations in the DM, DN, NM, and NN groups were 24.7 pg/ml, 15.3 pg/ml, 24.1 pg/ml, and 12.9 pg/ml, respectively. The concentrations of C3a and C5a in the DM and NM groups were significantly higher than in the NN group (P < 0.05 for both comparisons). The CAPs in the tear fluid in MGD and DE increased. Local dysregulation of the innate immune system can be associated with the development of MGD and DE in elderly patients. |
| first_indexed | 2024-03-08T16:20:31Z |
| format | Article |
| id | doaj.art-7fd469cdb84741d7860052cbe7ce5d77 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T16:20:31Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7fd469cdb84741d7860052cbe7ce5d772024-01-07T12:23:58ZengNature PortfolioScientific Reports2045-23222024-01-011411710.1038/s41598-023-46634-7Complement activation products in tears of dry eye and meibomian gland dysfunctionHiroki Maehara0Koki Norikawa1Keiichiro Tanaka2Yutaka Kato3Akihito Kasai4Ryo Mukai5Tomoko Omori6Takeshi Machida7Hideharu Sekine8Tetsuju Sekiryu9Department of Ophthalmology, Fukushima Medical University School of MedicineDepartment of Ophthalmology, Fukushima Medical University School of MedicineDepartment of Ophthalmology, Fukushima Medical University School of MedicineDepartment of Ophthalmology, Fukushima Medical University School of MedicineDepartment of Ophthalmology, Fukushima Medical University School of MedicineDepartment of Ophthalmology, Fukushima Medical University School of MedicineDepartment of Immunology, Fukushima Medical University School of MedicineDepartment of Immunology, Fukushima Medical University School of MedicineDepartment of Immunology, Fukushima Medical University School of MedicineDepartment of Ophthalmology, Fukushima Medical University School of MedicineAbstract Sixty-seven patients (38 woman; median age, 69 years) were enrolled to assess complement activation products (CAPs) in tear fluid with/without dry eye (DE) and with/without meibomian gland dysfunction (MGD). Patients were divided into four groups based on the presence/absence of DE and MGD: group DM had both DE and MGD, group DN had DE without MGD, group NM had MGD without DE, and group NN had neither DE nor MGD. The levels of C3a and C5a in the collected tears were analyzed using a cytometric bead array. The C3a concentrations in the DM, DN, NM, and NN groups were 2326 pg/ml, 1411 pg/ml, 1821 pg/ml, and 978 pg/ml, respectively. The C5a concentrations in the DM, DN, NM, and NN groups were 24.7 pg/ml, 15.3 pg/ml, 24.1 pg/ml, and 12.9 pg/ml, respectively. The concentrations of C3a and C5a in the DM and NM groups were significantly higher than in the NN group (P < 0.05 for both comparisons). The CAPs in the tear fluid in MGD and DE increased. Local dysregulation of the innate immune system can be associated with the development of MGD and DE in elderly patients.https://doi.org/10.1038/s41598-023-46634-7 |
| spellingShingle | Hiroki Maehara Koki Norikawa Keiichiro Tanaka Yutaka Kato Akihito Kasai Ryo Mukai Tomoko Omori Takeshi Machida Hideharu Sekine Tetsuju Sekiryu Complement activation products in tears of dry eye and meibomian gland dysfunction Scientific Reports |
| title | Complement activation products in tears of dry eye and meibomian gland dysfunction |
| title_full | Complement activation products in tears of dry eye and meibomian gland dysfunction |
| title_fullStr | Complement activation products in tears of dry eye and meibomian gland dysfunction |
| title_full_unstemmed | Complement activation products in tears of dry eye and meibomian gland dysfunction |
| title_short | Complement activation products in tears of dry eye and meibomian gland dysfunction |
| title_sort | complement activation products in tears of dry eye and meibomian gland dysfunction |
| url | https://doi.org/10.1038/s41598-023-46634-7 |
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