circFBLIM1 act as a ceRNA to promote hepatocellular cancer progression by sponging miR-346
Abstract Backgroud Accumulating evidences indicate that circular RNAs (circRNAs), a class of non-coding RNAs, play important roles in tumorigenesis. However, the function of circRNAs in hepatocellular cancer (HCC) is largely unknown. Methods We performed circRNA microarrays to identify circRNAs that...
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| Format: | Article |
| Language: | English |
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BMC
2018-07-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13046-018-0838-8 |
| _version_ | 1819237850637402112 |
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| author | Ning Bai Eming Peng Xingsheng Qiu Ning Lyu Zhejia Zhang Yiming Tao Xinying Li Zhiming Wang |
| author_facet | Ning Bai Eming Peng Xingsheng Qiu Ning Lyu Zhejia Zhang Yiming Tao Xinying Li Zhiming Wang |
| author_sort | Ning Bai |
| collection | DOAJ |
| description | Abstract Backgroud Accumulating evidences indicate that circular RNAs (circRNAs), a class of non-coding RNAs, play important roles in tumorigenesis. However, the function of circRNAs in hepatocellular cancer (HCC) is largely unknown. Methods We performed circRNA microarrays to identify circRNAs that are aberrantly expressed in HCC tissues. Expression levels of a significantly upregulated circRNA, circFBLIM1, was detected by quantitative real-time PCR (qRT-PCR) in HCC cell lines and tissues. Then, we examined the functions of circFBLIM1 in HCC by cell proliferation, apoptosis, invasion and mouse xenograft assay. In addition, luciferase assay and RNA immunoprecipitation (RIP) assay were used to explore the miRNA sponge function of circFBLIM1 in HCC. Results Microarray analysis and qRT-PCR verified a circRNA termed circFBLIM1 that was upregulated in HCC tissues and cell lines. Knockdown of circFBLIM1 inhibited proliferation, invasion and promoted apoptosis in HCC. Via luciferase reporter assays, circFBLIM1 and FBLIM1 were observed to directly bind to miR-346. Subsequent experiments showed that circFBLIM1 and FBLIM1 regulated the expression of each other by sponging miR-346. Conclusions Taken together, we conclude that circFBLIM1 may function as a competing endogenous RNA (ceRNA) to regulate FBLIM1 expression through sponging miR-346 to exert regulatory functions in HCC. circFBLIM1 may be a diagnostic biomarker and potential target for HCC therapy. |
| first_indexed | 2024-12-23T13:26:53Z |
| format | Article |
| id | doaj.art-7fd4e319d555430dbc5b49bf8cbda4c3 |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-12-23T13:26:53Z |
| publishDate | 2018-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-7fd4e319d555430dbc5b49bf8cbda4c32022-12-21T17:45:16ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662018-07-013711910.1186/s13046-018-0838-8circFBLIM1 act as a ceRNA to promote hepatocellular cancer progression by sponging miR-346Ning Bai0Eming Peng1Xingsheng Qiu2Ning Lyu3Zhejia Zhang4Yiming Tao5Xinying Li6Zhiming Wang7Department of General Surgery, Xiangya Hospital, Central South UniversityDepartment of XIMC Outpatient, Xiangya Hospital, Central South UniversityDepartment of Radiation Oncology, Sun Yat-Sen Memorial Hospital, Sun Yat-sen UniversityDepartment of Medical Imaging and Interventional Radiology, Sun Yat-sen University Cancer CenterDepartment of General Surgery, Xiangya Hospital, Central South UniversityDepartment of General Surgery, Xiangya Hospital, Central South UniversityDepartment of General Surgery, Xiangya Hospital, Central South UniversityDepartment of General Surgery, Xiangya Hospital, Central South UniversityAbstract Backgroud Accumulating evidences indicate that circular RNAs (circRNAs), a class of non-coding RNAs, play important roles in tumorigenesis. However, the function of circRNAs in hepatocellular cancer (HCC) is largely unknown. Methods We performed circRNA microarrays to identify circRNAs that are aberrantly expressed in HCC tissues. Expression levels of a significantly upregulated circRNA, circFBLIM1, was detected by quantitative real-time PCR (qRT-PCR) in HCC cell lines and tissues. Then, we examined the functions of circFBLIM1 in HCC by cell proliferation, apoptosis, invasion and mouse xenograft assay. In addition, luciferase assay and RNA immunoprecipitation (RIP) assay were used to explore the miRNA sponge function of circFBLIM1 in HCC. Results Microarray analysis and qRT-PCR verified a circRNA termed circFBLIM1 that was upregulated in HCC tissues and cell lines. Knockdown of circFBLIM1 inhibited proliferation, invasion and promoted apoptosis in HCC. Via luciferase reporter assays, circFBLIM1 and FBLIM1 were observed to directly bind to miR-346. Subsequent experiments showed that circFBLIM1 and FBLIM1 regulated the expression of each other by sponging miR-346. Conclusions Taken together, we conclude that circFBLIM1 may function as a competing endogenous RNA (ceRNA) to regulate FBLIM1 expression through sponging miR-346 to exert regulatory functions in HCC. circFBLIM1 may be a diagnostic biomarker and potential target for HCC therapy.http://link.springer.com/article/10.1186/s13046-018-0838-8Circular RNAsmiR-346FBLIM1Competitive endogenous RNAsHepatocellular cancer |
| spellingShingle | Ning Bai Eming Peng Xingsheng Qiu Ning Lyu Zhejia Zhang Yiming Tao Xinying Li Zhiming Wang circFBLIM1 act as a ceRNA to promote hepatocellular cancer progression by sponging miR-346 Journal of Experimental & Clinical Cancer Research Circular RNAs miR-346 FBLIM1 Competitive endogenous RNAs Hepatocellular cancer |
| title | circFBLIM1 act as a ceRNA to promote hepatocellular cancer progression by sponging miR-346 |
| title_full | circFBLIM1 act as a ceRNA to promote hepatocellular cancer progression by sponging miR-346 |
| title_fullStr | circFBLIM1 act as a ceRNA to promote hepatocellular cancer progression by sponging miR-346 |
| title_full_unstemmed | circFBLIM1 act as a ceRNA to promote hepatocellular cancer progression by sponging miR-346 |
| title_short | circFBLIM1 act as a ceRNA to promote hepatocellular cancer progression by sponging miR-346 |
| title_sort | circfblim1 act as a cerna to promote hepatocellular cancer progression by sponging mir 346 |
| topic | Circular RNAs miR-346 FBLIM1 Competitive endogenous RNAs Hepatocellular cancer |
| url | http://link.springer.com/article/10.1186/s13046-018-0838-8 |
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