RETRACTED ARTICLE: Circular RNA hsa_circ_0001829 promotes gastric cancer progression through miR-155-5p/SMAD2 axis
Abstract Background Accumulating evidences have shown that circular RNAs (circRNAs) play important roles in regulating the pathogenesis of cancer. However, the role of circRNAs in gastric cancer (GC) remains largely unclear. Methods In this study, we identified a novel upregulated circRNA, hsa_circ_...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-12-01
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| Series: | Journal of Experimental & Clinical Cancer Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13046-020-01790-w |
| _version_ | 1827956526316257280 |
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| author | Qiuling Niu Zhijie Dong Min Liang Yuanwei Luo Hai Lin Mingzhen Lin Xiu Zhong Wenxia Yao Jinsheng Weng Xinke Zhou |
| author_facet | Qiuling Niu Zhijie Dong Min Liang Yuanwei Luo Hai Lin Mingzhen Lin Xiu Zhong Wenxia Yao Jinsheng Weng Xinke Zhou |
| author_sort | Qiuling Niu |
| collection | DOAJ |
| description | Abstract Background Accumulating evidences have shown that circular RNAs (circRNAs) play important roles in regulating the pathogenesis of cancer. However, the role of circRNAs in gastric cancer (GC) remains largely unclear. Methods In this study, we identified a novel upregulated circRNA, hsa_circ_0001829, in chemically induced malignant transformed human gastric epithelial cells using RNA-seq. Subsequent qRT-PCR and ISH assays were performed to detect the expression level of hsa_circ_0001829 in GC cell lines and tissues. Functional roles of hsa_circ_0001829 in GC were then explored by loss- and gain-of- function assays. Bioinformatic prediction and luciferase assay were used to investigate potential mechanisms of hsa_circ_0001829. Finally, the mice xenograft and metastasis models were constructed to assess the function of hsa_circ_0001829 in vivo. Results We found that hsa_circ_0001829 was significantly upregulated in GC tissues and cell lines. Loss- and gain-of- function assays showed that hsa_circ_0001829 promotes GC cells proliferation, migration and invasion, and the affected cell cycle progression and apoptosis rates may account for the effect of hsa_circ_0001829 on GC proliferation. In addition, bioinformatic prediction and luciferase assay showed that hsa_circ_0001829 acts as a molecular sponge for miR-155-5p and that SMAD2 was a target gene of miR-155-5p; moreover, hsa_circ_0001829 sponges miR-155-5p to regulate SMAD2 expression and hsa_circ_0001829 promotes GC progression through the miR-155-5p–SMAD2 pathway. Finally, suppression of hsa_circ_0001829 expression inhibited tumor growth and aggressiveness in vivo. Conclusion Taken together, our findings firstly demonstrated a novel oncogenic role of hsa_circ_0001829 in GC progression through miR-155-5p–SMAD2 axis, and our study may offer novel biomarkers and therapeutic targets for GC. |
| first_indexed | 2024-04-09T15:05:15Z |
| format | Article |
| id | doaj.art-7fd8c5d93a7f4782853fd118aecd5a08 |
| institution | Directory Open Access Journal |
| issn | 1756-9966 |
| language | English |
| last_indexed | 2024-04-09T15:05:15Z |
| publishDate | 2020-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Experimental & Clinical Cancer Research |
| spelling | doaj.art-7fd8c5d93a7f4782853fd118aecd5a082023-04-30T11:31:55ZengBMCJournal of Experimental & Clinical Cancer Research1756-99662020-12-0139111710.1186/s13046-020-01790-wRETRACTED ARTICLE: Circular RNA hsa_circ_0001829 promotes gastric cancer progression through miR-155-5p/SMAD2 axisQiuling Niu0Zhijie Dong1Min Liang2Yuanwei Luo3Hai Lin4Mingzhen Lin5Xiu Zhong6Wenxia Yao7Jinsheng Weng8Xinke Zhou9The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical UniversityThe Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical UniversityThe Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical UniversityThe Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical UniversityThe Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical UniversityThe Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical UniversityThe Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical UniversityThe Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical UniversityThe Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical UniversityThe Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical UniversityAbstract Background Accumulating evidences have shown that circular RNAs (circRNAs) play important roles in regulating the pathogenesis of cancer. However, the role of circRNAs in gastric cancer (GC) remains largely unclear. Methods In this study, we identified a novel upregulated circRNA, hsa_circ_0001829, in chemically induced malignant transformed human gastric epithelial cells using RNA-seq. Subsequent qRT-PCR and ISH assays were performed to detect the expression level of hsa_circ_0001829 in GC cell lines and tissues. Functional roles of hsa_circ_0001829 in GC were then explored by loss- and gain-of- function assays. Bioinformatic prediction and luciferase assay were used to investigate potential mechanisms of hsa_circ_0001829. Finally, the mice xenograft and metastasis models were constructed to assess the function of hsa_circ_0001829 in vivo. Results We found that hsa_circ_0001829 was significantly upregulated in GC tissues and cell lines. Loss- and gain-of- function assays showed that hsa_circ_0001829 promotes GC cells proliferation, migration and invasion, and the affected cell cycle progression and apoptosis rates may account for the effect of hsa_circ_0001829 on GC proliferation. In addition, bioinformatic prediction and luciferase assay showed that hsa_circ_0001829 acts as a molecular sponge for miR-155-5p and that SMAD2 was a target gene of miR-155-5p; moreover, hsa_circ_0001829 sponges miR-155-5p to regulate SMAD2 expression and hsa_circ_0001829 promotes GC progression through the miR-155-5p–SMAD2 pathway. Finally, suppression of hsa_circ_0001829 expression inhibited tumor growth and aggressiveness in vivo. Conclusion Taken together, our findings firstly demonstrated a novel oncogenic role of hsa_circ_0001829 in GC progression through miR-155-5p–SMAD2 axis, and our study may offer novel biomarkers and therapeutic targets for GC.https://doi.org/10.1186/s13046-020-01790-wGastric cancerProgressionhsa_circ_0001829miR-155-5pSMAD2 |
| spellingShingle | Qiuling Niu Zhijie Dong Min Liang Yuanwei Luo Hai Lin Mingzhen Lin Xiu Zhong Wenxia Yao Jinsheng Weng Xinke Zhou RETRACTED ARTICLE: Circular RNA hsa_circ_0001829 promotes gastric cancer progression through miR-155-5p/SMAD2 axis Journal of Experimental & Clinical Cancer Research Gastric cancer Progression hsa_circ_0001829 miR-155-5p SMAD2 |
| title | RETRACTED ARTICLE: Circular RNA hsa_circ_0001829 promotes gastric cancer progression through miR-155-5p/SMAD2 axis |
| title_full | RETRACTED ARTICLE: Circular RNA hsa_circ_0001829 promotes gastric cancer progression through miR-155-5p/SMAD2 axis |
| title_fullStr | RETRACTED ARTICLE: Circular RNA hsa_circ_0001829 promotes gastric cancer progression through miR-155-5p/SMAD2 axis |
| title_full_unstemmed | RETRACTED ARTICLE: Circular RNA hsa_circ_0001829 promotes gastric cancer progression through miR-155-5p/SMAD2 axis |
| title_short | RETRACTED ARTICLE: Circular RNA hsa_circ_0001829 promotes gastric cancer progression through miR-155-5p/SMAD2 axis |
| title_sort | retracted article circular rna hsa circ 0001829 promotes gastric cancer progression through mir 155 5p smad2 axis |
| topic | Gastric cancer Progression hsa_circ_0001829 miR-155-5p SMAD2 |
| url | https://doi.org/10.1186/s13046-020-01790-w |
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