Genome-Wide Identification and Analysis Uncovers the Potential Role of JAZ and MYC Families in Potato under Abiotic Stress
As key regulators of the Jasmonates (JAs) signal transduction pathway, JAZ protein, and MYC transcription factors are imperative for plant response to external environmental changes, growth, and development. In this study, 18 <i>StJAZs</i> and 12 <i>StMYCs</i> were identified...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/7/6706 |
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| author | Shan Wang Yongbin Wang Rui Yang Wanhua Cai Yaning Liu Duanrong Zhou Li Meng Ping Wang Binquan Huang |
| author_facet | Shan Wang Yongbin Wang Rui Yang Wanhua Cai Yaning Liu Duanrong Zhou Li Meng Ping Wang Binquan Huang |
| author_sort | Shan Wang |
| collection | DOAJ |
| description | As key regulators of the Jasmonates (JAs) signal transduction pathway, JAZ protein, and MYC transcription factors are imperative for plant response to external environmental changes, growth, and development. In this study, 18 <i>StJAZs</i> and 12 <i>StMYCs</i> were identified in potatoes. Their chromosomal position, phylogenetic development, gene structure, and promoter cis-acting parts of the <i>StJAZ</i> genes were analyzed. In addition, Protein–Protein Interaction (PPI) network analysis of <i>StJAZ</i> and <i>StMYC</i> gene families and yeast two-hybrid assay demonstrated that five StMYCs can interact with 16 StJAZs, which provides new insights into the operation mechanism of StJAZs and StMYCs in JA signal response. Moreover, we explored the expression profiles of StJAZs and StMYCs genes in different tissues and during abiotic stresses by RNA-seq data. Based on the PPI network and transcriptome data, the genes <i>StJAZ11</i>, <i>StJAZ16</i>, and <i>StMYC6</i> were chosen for further qRT-PCR study under salt or mannitol treatment. Under mannitol-induced drought or salinity treatment, the expression patterns of <i>StMYC6</i>, <i>StJAZ11</i>, and <i>StJAZ16</i> were different, indicating that the JAZ protein and MYC transcription factor may be engaged in the response of potatoes to abiotic stress, which opened up a new research direction for the genetic improvement of potatoes in response to environmental stress. |
| first_indexed | 2024-03-11T05:33:52Z |
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| id | doaj.art-7fda7a1d3d074960b000b584afed8f72 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T05:33:52Z |
| publishDate | 2023-04-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7fda7a1d3d074960b000b584afed8f722023-11-17T16:54:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01247670610.3390/ijms24076706Genome-Wide Identification and Analysis Uncovers the Potential Role of JAZ and MYC Families in Potato under Abiotic StressShan Wang0Yongbin Wang1Rui Yang2Wanhua Cai3Yaning Liu4Duanrong Zhou5Li Meng6Ping Wang7Binquan Huang8State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Agriculture, Yunnan University, Kunming 650504, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Agriculture, Yunnan University, Kunming 650504, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Agriculture, Yunnan University, Kunming 650504, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Agriculture, Yunnan University, Kunming 650504, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Agriculture, Yunnan University, Kunming 650504, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Agriculture, Yunnan University, Kunming 650504, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Agriculture, Yunnan University, Kunming 650504, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Agriculture, Yunnan University, Kunming 650504, ChinaState Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Agriculture, Yunnan University, Kunming 650504, ChinaAs key regulators of the Jasmonates (JAs) signal transduction pathway, JAZ protein, and MYC transcription factors are imperative for plant response to external environmental changes, growth, and development. In this study, 18 <i>StJAZs</i> and 12 <i>StMYCs</i> were identified in potatoes. Their chromosomal position, phylogenetic development, gene structure, and promoter cis-acting parts of the <i>StJAZ</i> genes were analyzed. In addition, Protein–Protein Interaction (PPI) network analysis of <i>StJAZ</i> and <i>StMYC</i> gene families and yeast two-hybrid assay demonstrated that five StMYCs can interact with 16 StJAZs, which provides new insights into the operation mechanism of StJAZs and StMYCs in JA signal response. Moreover, we explored the expression profiles of StJAZs and StMYCs genes in different tissues and during abiotic stresses by RNA-seq data. Based on the PPI network and transcriptome data, the genes <i>StJAZ11</i>, <i>StJAZ16</i>, and <i>StMYC6</i> were chosen for further qRT-PCR study under salt or mannitol treatment. Under mannitol-induced drought or salinity treatment, the expression patterns of <i>StMYC6</i>, <i>StJAZ11</i>, and <i>StJAZ16</i> were different, indicating that the JAZ protein and MYC transcription factor may be engaged in the response of potatoes to abiotic stress, which opened up a new research direction for the genetic improvement of potatoes in response to environmental stress.https://www.mdpi.com/1422-0067/24/7/6706JAZ familyMYC familypotatoprotein interactionabiotic stress |
| spellingShingle | Shan Wang Yongbin Wang Rui Yang Wanhua Cai Yaning Liu Duanrong Zhou Li Meng Ping Wang Binquan Huang Genome-Wide Identification and Analysis Uncovers the Potential Role of JAZ and MYC Families in Potato under Abiotic Stress International Journal of Molecular Sciences JAZ family MYC family potato protein interaction abiotic stress |
| title | Genome-Wide Identification and Analysis Uncovers the Potential Role of JAZ and MYC Families in Potato under Abiotic Stress |
| title_full | Genome-Wide Identification and Analysis Uncovers the Potential Role of JAZ and MYC Families in Potato under Abiotic Stress |
| title_fullStr | Genome-Wide Identification and Analysis Uncovers the Potential Role of JAZ and MYC Families in Potato under Abiotic Stress |
| title_full_unstemmed | Genome-Wide Identification and Analysis Uncovers the Potential Role of JAZ and MYC Families in Potato under Abiotic Stress |
| title_short | Genome-Wide Identification and Analysis Uncovers the Potential Role of JAZ and MYC Families in Potato under Abiotic Stress |
| title_sort | genome wide identification and analysis uncovers the potential role of jaz and myc families in potato under abiotic stress |
| topic | JAZ family MYC family potato protein interaction abiotic stress |
| url | https://www.mdpi.com/1422-0067/24/7/6706 |
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