Enhanced production, purification and biochemical characterization of therapeutic potential fibrinolytic enzyme from a new Bacillus flexus from marine environment
Objectives: The main aim of this study is to isolate and characterize fibrinolytic enzyme from Bacillus flexus. Methods: Fish meal of Sardinella longiceps and anchovy was optimized using a two-level full factorial design (25) and response surface methodology. The significant physical factors and nut...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-10-01
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| Series: | Journal of King Saud University: Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364720302718 |
| _version_ | 1828529463668768768 |
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| author | Dunia A. Al Farraj T. Sujin Jeba Kumar Ponnuswamy Vijayaraghavan Mohamed Soliman Elshikh Roua M. Alkufeidy Noorah A. Alkubaisi Maryam K. Alshammari |
| author_facet | Dunia A. Al Farraj T. Sujin Jeba Kumar Ponnuswamy Vijayaraghavan Mohamed Soliman Elshikh Roua M. Alkufeidy Noorah A. Alkubaisi Maryam K. Alshammari |
| author_sort | Dunia A. Al Farraj |
| collection | DOAJ |
| description | Objectives: The main aim of this study is to isolate and characterize fibrinolytic enzyme from Bacillus flexus. Methods: Fish meal of Sardinella longiceps and anchovy was optimized using a two-level full factorial design (25) and response surface methodology. The significant physical factors and nutrient sources (peptone, maltose, and magnesium chloride) were identified by statistical approach. The properties of a purified enzyme including their effect at different temperature, pH and the effect of metal ions were evaluated. Results: Enzyme yield was improved 3.5 fold than unoptimized medium. Central composite design optimized culture medium enhanced enzyme yield (4711 ± 29.3 U/g of substrate). The fibrinolytic enzyme was highly active at alkaline pH (8.0), 50 °C and the molecular weight was 32 kDa. Conclusions: From these findings, it concludes that this fibrinolytic enzyme could be a novel potent thrombolytic agent. |
| first_indexed | 2024-12-11T22:09:31Z |
| format | Article |
| id | doaj.art-7fdc2697993e4009b5a22b3843e6413a |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-12-11T22:09:31Z |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-7fdc2697993e4009b5a22b3843e6413a2022-12-22T00:48:51ZengElsevierJournal of King Saud University: Science1018-36472020-10-0132731743180Enhanced production, purification and biochemical characterization of therapeutic potential fibrinolytic enzyme from a new Bacillus flexus from marine environmentDunia A. Al Farraj0T. Sujin Jeba Kumar1Ponnuswamy Vijayaraghavan2Mohamed Soliman Elshikh3Roua M. Alkufeidy4Noorah A. Alkubaisi5Maryam K. Alshammari6Department of Botany and Microbiology, College of Sciences, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi Arabia; Corresponding author.Bioprocess Engineering Division, Smykon Biotech Pvt LtD, Nagercoil, Kanyakumari, Tamil Nadu 629201, IndiaBioprocess Engineering Division, Smykon Biotech Pvt LtD, Nagercoil, Kanyakumari, Tamil Nadu 629201, IndiaDepartment of Botany and Microbiology, College of Sciences, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaDepartment of Botany and Microbiology, College of Sciences, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaDepartment of Botany and Microbiology, College of Sciences, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaDepartment of Botany and Microbiology, College of Sciences, King Saud University, P.O. Box 22452, Riyadh 11495, Saudi ArabiaObjectives: The main aim of this study is to isolate and characterize fibrinolytic enzyme from Bacillus flexus. Methods: Fish meal of Sardinella longiceps and anchovy was optimized using a two-level full factorial design (25) and response surface methodology. The significant physical factors and nutrient sources (peptone, maltose, and magnesium chloride) were identified by statistical approach. The properties of a purified enzyme including their effect at different temperature, pH and the effect of metal ions were evaluated. Results: Enzyme yield was improved 3.5 fold than unoptimized medium. Central composite design optimized culture medium enhanced enzyme yield (4711 ± 29.3 U/g of substrate). The fibrinolytic enzyme was highly active at alkaline pH (8.0), 50 °C and the molecular weight was 32 kDa. Conclusions: From these findings, it concludes that this fibrinolytic enzyme could be a novel potent thrombolytic agent.http://www.sciencedirect.com/science/article/pii/S1018364720302718Fibrinolytic enzymeBacillusCheap substratesBlood clotFibrinolytic agent |
| spellingShingle | Dunia A. Al Farraj T. Sujin Jeba Kumar Ponnuswamy Vijayaraghavan Mohamed Soliman Elshikh Roua M. Alkufeidy Noorah A. Alkubaisi Maryam K. Alshammari Enhanced production, purification and biochemical characterization of therapeutic potential fibrinolytic enzyme from a new Bacillus flexus from marine environment Journal of King Saud University: Science Fibrinolytic enzyme Bacillus Cheap substrates Blood clot Fibrinolytic agent |
| title | Enhanced production, purification and biochemical characterization of therapeutic potential fibrinolytic enzyme from a new Bacillus flexus from marine environment |
| title_full | Enhanced production, purification and biochemical characterization of therapeutic potential fibrinolytic enzyme from a new Bacillus flexus from marine environment |
| title_fullStr | Enhanced production, purification and biochemical characterization of therapeutic potential fibrinolytic enzyme from a new Bacillus flexus from marine environment |
| title_full_unstemmed | Enhanced production, purification and biochemical characterization of therapeutic potential fibrinolytic enzyme from a new Bacillus flexus from marine environment |
| title_short | Enhanced production, purification and biochemical characterization of therapeutic potential fibrinolytic enzyme from a new Bacillus flexus from marine environment |
| title_sort | enhanced production purification and biochemical characterization of therapeutic potential fibrinolytic enzyme from a new bacillus flexus from marine environment |
| topic | Fibrinolytic enzyme Bacillus Cheap substrates Blood clot Fibrinolytic agent |
| url | http://www.sciencedirect.com/science/article/pii/S1018364720302718 |
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