The Role of a Soybean 14-3-3 Gene (Glyma05g29080) on White Mold Resistance and Nodulation Investigations Using CRISPR-Cas9 Editing and RNA Silencing
The role of a soybean 14-3-3 gene (Glyma05g29080) in defense against white mold and in nodulation was investigated by loss-of-gene-function with CRISPR-Cas9 editing and silencing of RNA interference (RNAi). Particle bombardment was used to introduce the CRISPR expression cassette to target the soybe...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2023-03-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-07-22-0157-R |
| _version_ | 1797871801122947072 |
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| author | Yunfang Zhang Laureen Blahut-Beatty Suqin Zheng Steven J. Clough Daina H. Simmonds |
| author_facet | Yunfang Zhang Laureen Blahut-Beatty Suqin Zheng Steven J. Clough Daina H. Simmonds |
| author_sort | Yunfang Zhang |
| collection | DOAJ |
| description | The role of a soybean 14-3-3 gene (Glyma05g29080) in defense against white mold and in nodulation was investigated by loss-of-gene-function with CRISPR-Cas9 editing and silencing of RNA interference (RNAi). Particle bombardment was used to introduce the CRISPR expression cassette to target the soybean 14-3-3 gene and an RNAi construct to silence gene transcription. Transmission of the edited 14-3-3 gene and the RNAi construct was confirmed in their respective progeny. The recovered transgenic plants and their progeny were significantly more susceptible to Sclerotinia sclerotiorum infection and showed a significant reduction in nodulation, thus confirming the role of the 14-3-3 gene (Glyma05g29080) in both nodulation and defense. |
| first_indexed | 2024-04-10T00:49:48Z |
| format | Article |
| id | doaj.art-7fdef33778a7466d826126b92fb9b377 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-10T00:49:48Z |
| publishDate | 2023-03-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-7fdef33778a7466d826126b92fb9b3772023-03-13T14:48:36ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062023-03-0136315916410.1094/MPMI-07-22-0157-RThe Role of a Soybean 14-3-3 Gene (Glyma05g29080) on White Mold Resistance and Nodulation Investigations Using CRISPR-Cas9 Editing and RNA SilencingYunfang Zhang0Laureen Blahut-Beatty1Suqin Zheng2Steven J. Clough3Daina H. Simmonds4Ottawa Research and Development Centre, Science and Technology, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, CanadaOttawa Research and Development Centre, Science and Technology, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, CanadaOttawa Research and Development Centre, Science and Technology, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, CanadaUnited States Department of Agriculture-Agricultural Research Service, Urbana, IL 61801, U.S.A.Ottawa Research and Development Centre, Science and Technology, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0C6, CanadaThe role of a soybean 14-3-3 gene (Glyma05g29080) in defense against white mold and in nodulation was investigated by loss-of-gene-function with CRISPR-Cas9 editing and silencing of RNA interference (RNAi). Particle bombardment was used to introduce the CRISPR expression cassette to target the soybean 14-3-3 gene and an RNAi construct to silence gene transcription. Transmission of the edited 14-3-3 gene and the RNAi construct was confirmed in their respective progeny. The recovered transgenic plants and their progeny were significantly more susceptible to Sclerotinia sclerotiorum infection and showed a significant reduction in nodulation, thus confirming the role of the 14-3-3 gene (Glyma05g29080) in both nodulation and defense.https://apsjournals.apsnet.org/doi/10.1094/MPMI-07-22-0157-R14-3-3CRISPR-Cas9nodulationplant defenseRNAiSclerotinia sclerotiorum |
| spellingShingle | Yunfang Zhang Laureen Blahut-Beatty Suqin Zheng Steven J. Clough Daina H. Simmonds The Role of a Soybean 14-3-3 Gene (Glyma05g29080) on White Mold Resistance and Nodulation Investigations Using CRISPR-Cas9 Editing and RNA Silencing Molecular Plant-Microbe Interactions 14-3-3 CRISPR-Cas9 nodulation plant defense RNAi Sclerotinia sclerotiorum |
| title | The Role of a Soybean 14-3-3 Gene (Glyma05g29080) on White Mold Resistance and Nodulation Investigations Using CRISPR-Cas9 Editing and RNA Silencing |
| title_full | The Role of a Soybean 14-3-3 Gene (Glyma05g29080) on White Mold Resistance and Nodulation Investigations Using CRISPR-Cas9 Editing and RNA Silencing |
| title_fullStr | The Role of a Soybean 14-3-3 Gene (Glyma05g29080) on White Mold Resistance and Nodulation Investigations Using CRISPR-Cas9 Editing and RNA Silencing |
| title_full_unstemmed | The Role of a Soybean 14-3-3 Gene (Glyma05g29080) on White Mold Resistance and Nodulation Investigations Using CRISPR-Cas9 Editing and RNA Silencing |
| title_short | The Role of a Soybean 14-3-3 Gene (Glyma05g29080) on White Mold Resistance and Nodulation Investigations Using CRISPR-Cas9 Editing and RNA Silencing |
| title_sort | role of a soybean 14 3 3 gene glyma05g29080 on white mold resistance and nodulation investigations using crispr cas9 editing and rna silencing |
| topic | 14-3-3 CRISPR-Cas9 nodulation plant defense RNAi Sclerotinia sclerotiorum |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-07-22-0157-R |
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