Low molecular weight chitosan oligosaccharides form stable complexes with human lactoferrin
Proteins in tears, including human lactoferrin (HLF), can be deposited and denatured on contact lenses, increasing the risk of microbial cell attachment to the lens and ocular complications. The surfactants currently used in commercial contact lens care solutions have low clearance ability for tear...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-12-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.13722 |
| _version_ | 1797402884482007040 |
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| author | Juan Li Shimin Guan Baoguo Cai Qianqian Li Shaofeng Rong |
| author_facet | Juan Li Shimin Guan Baoguo Cai Qianqian Li Shaofeng Rong |
| author_sort | Juan Li |
| collection | DOAJ |
| description | Proteins in tears, including human lactoferrin (HLF), can be deposited and denatured on contact lenses, increasing the risk of microbial cell attachment to the lens and ocular complications. The surfactants currently used in commercial contact lens care solutions have low clearance ability for tear proteins. Chitosan oligosaccharide (COS) binds to a variety of proteins and has potential for use in protein removal, especially in contact lens care solutions. Here, we analyzed the interaction mechanism of COSs hydrolyzed from chitosan from different resources with HLF. The molecular weights (MWs) and concentrations of COSs were key factors for the formation of COS–HLF complexes. Lower MWs of COSs could form more stable COS–HLF complexes. COS from Aspergillus ochraceus had a superior effect on HLF compared with COS from shrimp and crab shell with the same MWs. In conclusion, COSs could bind to and cause a conformational change in HLF. Therefore, COSs, especially those with low MWs, have potential as deproteinizing agents in contact lens care solution. |
| first_indexed | 2024-03-09T02:30:40Z |
| format | Article |
| id | doaj.art-7fe1485bbe4f48dabf44d3de49c2b1df |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-03-09T02:30:40Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-7fe1485bbe4f48dabf44d3de49c2b1df2023-12-06T13:08:18ZengWileyFEBS Open Bio2211-54632023-12-0113122215222310.1002/2211-5463.13722Low molecular weight chitosan oligosaccharides form stable complexes with human lactoferrinJuan Li0Shimin Guan1Baoguo Cai2Qianqian Li3Shaofeng Rong4Department of Bioengineering, School of Perfume and Aroma Technology Shanghai Institute of Technology Shanghai ChinaDepartment of Bioengineering, School of Perfume and Aroma Technology Shanghai Institute of Technology Shanghai ChinaDepartment of Bioengineering, School of Perfume and Aroma Technology Shanghai Institute of Technology Shanghai ChinaDepartment of Bioengineering, School of Perfume and Aroma Technology Shanghai Institute of Technology Shanghai ChinaDepartment of Bioengineering, School of Perfume and Aroma Technology Shanghai Institute of Technology Shanghai ChinaProteins in tears, including human lactoferrin (HLF), can be deposited and denatured on contact lenses, increasing the risk of microbial cell attachment to the lens and ocular complications. The surfactants currently used in commercial contact lens care solutions have low clearance ability for tear proteins. Chitosan oligosaccharide (COS) binds to a variety of proteins and has potential for use in protein removal, especially in contact lens care solutions. Here, we analyzed the interaction mechanism of COSs hydrolyzed from chitosan from different resources with HLF. The molecular weights (MWs) and concentrations of COSs were key factors for the formation of COS–HLF complexes. Lower MWs of COSs could form more stable COS–HLF complexes. COS from Aspergillus ochraceus had a superior effect on HLF compared with COS from shrimp and crab shell with the same MWs. In conclusion, COSs could bind to and cause a conformational change in HLF. Therefore, COSs, especially those with low MWs, have potential as deproteinizing agents in contact lens care solution.https://doi.org/10.1002/2211-5463.13722chitosan oligosaccharidefluorescence quenchinghuman lactoferrininteractionmolecular dockingUV–Vis spectra |
| spellingShingle | Juan Li Shimin Guan Baoguo Cai Qianqian Li Shaofeng Rong Low molecular weight chitosan oligosaccharides form stable complexes with human lactoferrin FEBS Open Bio chitosan oligosaccharide fluorescence quenching human lactoferrin interaction molecular docking UV–Vis spectra |
| title | Low molecular weight chitosan oligosaccharides form stable complexes with human lactoferrin |
| title_full | Low molecular weight chitosan oligosaccharides form stable complexes with human lactoferrin |
| title_fullStr | Low molecular weight chitosan oligosaccharides form stable complexes with human lactoferrin |
| title_full_unstemmed | Low molecular weight chitosan oligosaccharides form stable complexes with human lactoferrin |
| title_short | Low molecular weight chitosan oligosaccharides form stable complexes with human lactoferrin |
| title_sort | low molecular weight chitosan oligosaccharides form stable complexes with human lactoferrin |
| topic | chitosan oligosaccharide fluorescence quenching human lactoferrin interaction molecular docking UV–Vis spectra |
| url | https://doi.org/10.1002/2211-5463.13722 |
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