YPEL3 Negatively Regulates Endometrial Function via the Wnt/β-Catenin Pathways during Early Pregnancy in Goats
In ruminants, the establishment of pregnancy requires a series of structural and functional changes in the endometrium under the action of hormones, thereby providing an optimal environment for the implantation of the embryo. In this study, we explored the molecular mechanism by which YPEL3 regulate...
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MDPI AG
2022-10-01
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| Online Access: | https://www.mdpi.com/2076-2615/12/21/2973 |
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| author | Jianguo Liu Rendong Qiu Ran Liu Pengjie Song Pengfei Lin Huatao Chen Dong Zhou Aihua Wang Yaping Jin |
| author_facet | Jianguo Liu Rendong Qiu Ran Liu Pengjie Song Pengfei Lin Huatao Chen Dong Zhou Aihua Wang Yaping Jin |
| author_sort | Jianguo Liu |
| collection | DOAJ |
| description | In ruminants, the establishment of pregnancy requires a series of structural and functional changes in the endometrium under the action of hormones, thereby providing an optimal environment for the implantation of the embryo. In this study, we explored the molecular mechanism by which YPEL3 regulates endometrial function during gestation in goats. We found YPEL3 expression was significantly downregulated during early gestation and that YPEL3 overexpression inhibited the expression of <i>ISG15</i>, but had no significant effects on the expression of <i>RSAD2</i> and <i>CXCL10</i> in goat endometrial epithelial cells (gEECs). In addition, YPEL3 silencing significantly inhibited PGF<sub>2α</sub> secretion and the expression of the prostaglandin synthesis-related rate-limiting enzyme-encoding genes <i>PGFS</i> and <i>PTGES</i>, with no significant effect on the expression of <i>PTGS1</i> and <i>PTGS2</i>. Moreover, YPEL3 inhibited the expression of vimentin and β-catenin and pretreatment of gEECs with the β-catenin activator CHIR99021 prevented a YPEL3-induced decrease in vimentin expression. Collectively, our findings confirm that, as a hormone-regulated factor, YPEL3 regulates endometrial function by inhibiting the Wnt/β-catenin signaling pathway and provide new insights for further clarification of the mechanism by which YPEL3 functions during early pregnancy in ruminants. |
| first_indexed | 2024-03-09T19:21:09Z |
| format | Article |
| id | doaj.art-7fe41c14833e43d6bc2abb32b27f8f9f |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T19:21:09Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-7fe41c14833e43d6bc2abb32b27f8f9f2023-11-24T03:24:52ZengMDPI AGAnimals2076-26152022-10-011221297310.3390/ani12212973YPEL3 Negatively Regulates Endometrial Function via the Wnt/β-Catenin Pathways during Early Pregnancy in GoatsJianguo Liu0Rendong Qiu1Ran Liu2Pengjie Song3Pengfei Lin4Huatao Chen5Dong Zhou6Aihua Wang7Yaping Jin8Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaIn ruminants, the establishment of pregnancy requires a series of structural and functional changes in the endometrium under the action of hormones, thereby providing an optimal environment for the implantation of the embryo. In this study, we explored the molecular mechanism by which YPEL3 regulates endometrial function during gestation in goats. We found YPEL3 expression was significantly downregulated during early gestation and that YPEL3 overexpression inhibited the expression of <i>ISG15</i>, but had no significant effects on the expression of <i>RSAD2</i> and <i>CXCL10</i> in goat endometrial epithelial cells (gEECs). In addition, YPEL3 silencing significantly inhibited PGF<sub>2α</sub> secretion and the expression of the prostaglandin synthesis-related rate-limiting enzyme-encoding genes <i>PGFS</i> and <i>PTGES</i>, with no significant effect on the expression of <i>PTGS1</i> and <i>PTGS2</i>. Moreover, YPEL3 inhibited the expression of vimentin and β-catenin and pretreatment of gEECs with the β-catenin activator CHIR99021 prevented a YPEL3-induced decrease in vimentin expression. Collectively, our findings confirm that, as a hormone-regulated factor, YPEL3 regulates endometrial function by inhibiting the Wnt/β-catenin signaling pathway and provide new insights for further clarification of the mechanism by which YPEL3 functions during early pregnancy in ruminants.https://www.mdpi.com/2076-2615/12/21/2973YPEL3hormonegoat endometrial epithelial cellsWnt/β-cateninendometrial function |
| spellingShingle | Jianguo Liu Rendong Qiu Ran Liu Pengjie Song Pengfei Lin Huatao Chen Dong Zhou Aihua Wang Yaping Jin YPEL3 Negatively Regulates Endometrial Function via the Wnt/β-Catenin Pathways during Early Pregnancy in Goats Animals YPEL3 hormone goat endometrial epithelial cells Wnt/β-catenin endometrial function |
| title | YPEL3 Negatively Regulates Endometrial Function via the Wnt/β-Catenin Pathways during Early Pregnancy in Goats |
| title_full | YPEL3 Negatively Regulates Endometrial Function via the Wnt/β-Catenin Pathways during Early Pregnancy in Goats |
| title_fullStr | YPEL3 Negatively Regulates Endometrial Function via the Wnt/β-Catenin Pathways during Early Pregnancy in Goats |
| title_full_unstemmed | YPEL3 Negatively Regulates Endometrial Function via the Wnt/β-Catenin Pathways during Early Pregnancy in Goats |
| title_short | YPEL3 Negatively Regulates Endometrial Function via the Wnt/β-Catenin Pathways during Early Pregnancy in Goats |
| title_sort | ypel3 negatively regulates endometrial function via the wnt β catenin pathways during early pregnancy in goats |
| topic | YPEL3 hormone goat endometrial epithelial cells Wnt/β-catenin endometrial function |
| url | https://www.mdpi.com/2076-2615/12/21/2973 |
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