GbWRKY1, a member of the WRKY transcription factor family identified from Gossypium barbadense, is involved in resistance to Verticillium wilt
WRKY transcription factors are involved in complex signalling processes that occur during stress responses. However, in contrast to the exploration of such mechanisms in model plants, far less research has focussed on the WRKY family in cotton. We previously identified GbWRKY1 in cotton (Gossypium b...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | http://dx.doi.org/10.1080/13102818.2019.1667873 |
| _version_ | 1811197772010881024 |
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| author | Xue Zhang Jianfeng Liu Lizhu Wu Zhaoyu Wang Shuling Zhang |
| author_facet | Xue Zhang Jianfeng Liu Lizhu Wu Zhaoyu Wang Shuling Zhang |
| author_sort | Xue Zhang |
| collection | DOAJ |
| description | WRKY transcription factors are involved in complex signalling processes that occur during stress responses. However, in contrast to the exploration of such mechanisms in model plants, far less research has focussed on the WRKY family in cotton. We previously identified GbWRKY1 in cotton (Gossypium barbadense), the expression of which is induced upon infection by Verticillium dahliae. Our current results indicate that this gene with three introns, has a group of cis-acting elements in its promoter that are associated with plant growth and development as well as responses to environmental stresses. Here, we used virus-induced gene silencing to characterize the role of GbWRKY1. Silencing in cotton simultaneously up-regulated salicylic acid (SA)-mediated responses and inhibited jasmonate (JA)-inducible and ethylene (ET)-inducible defences, resulting in an increased susceptibility to V. dahliae. However, when compared with wild-type Arabidopsis, transgenic plants exhibited more resistance to infection with V. dahliae. In the latter genotype, expression was increased for pathogen-related PR4 and ACS2, two genes involved in the JA pathway and ET biosynthesis, while expression of PR5, involved in the SA pathway, decreased after V. dahliae infection. Moreover, POD activity and lignin content related to ethylene synthesis in transgenic Arabidopsis were higher than those in wild-type Arabidopsis after V. dahliae infection. Collectively, these findings suggest that GbWRKY1 is regulated by signalling pathways for SA, JA and ET, and mainly involved in resistance to V. dahliae attack by ET signalling pathway the response. This highlights the importance of WRKYs in regulating pathogen-induced responses and plant development. |
| first_indexed | 2024-04-12T01:19:49Z |
| format | Article |
| id | doaj.art-7fe463addcb14a71bc434803934e34e8 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-04-12T01:19:49Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-7fe463addcb14a71bc434803934e34e82022-12-22T03:53:50ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302019-01-013311354136410.1080/13102818.2019.16678731667873GbWRKY1, a member of the WRKY transcription factor family identified from Gossypium barbadense, is involved in resistance to Verticillium wiltXue Zhang0Jianfeng Liu1Lizhu Wu2Zhaoyu Wang3Shuling Zhang4Hebei UniversityHebei UniversityAgriculture University of HebeiHebei UniversityHebei UniversityWRKY transcription factors are involved in complex signalling processes that occur during stress responses. However, in contrast to the exploration of such mechanisms in model plants, far less research has focussed on the WRKY family in cotton. We previously identified GbWRKY1 in cotton (Gossypium barbadense), the expression of which is induced upon infection by Verticillium dahliae. Our current results indicate that this gene with three introns, has a group of cis-acting elements in its promoter that are associated with plant growth and development as well as responses to environmental stresses. Here, we used virus-induced gene silencing to characterize the role of GbWRKY1. Silencing in cotton simultaneously up-regulated salicylic acid (SA)-mediated responses and inhibited jasmonate (JA)-inducible and ethylene (ET)-inducible defences, resulting in an increased susceptibility to V. dahliae. However, when compared with wild-type Arabidopsis, transgenic plants exhibited more resistance to infection with V. dahliae. In the latter genotype, expression was increased for pathogen-related PR4 and ACS2, two genes involved in the JA pathway and ET biosynthesis, while expression of PR5, involved in the SA pathway, decreased after V. dahliae infection. Moreover, POD activity and lignin content related to ethylene synthesis in transgenic Arabidopsis were higher than those in wild-type Arabidopsis after V. dahliae infection. Collectively, these findings suggest that GbWRKY1 is regulated by signalling pathways for SA, JA and ET, and mainly involved in resistance to V. dahliae attack by ET signalling pathway the response. This highlights the importance of WRKYs in regulating pathogen-induced responses and plant development.http://dx.doi.org/10.1080/13102818.2019.1667873gbwrky1wrky transcription factorscottonverticillium wiltdisease resistance |
| spellingShingle | Xue Zhang Jianfeng Liu Lizhu Wu Zhaoyu Wang Shuling Zhang GbWRKY1, a member of the WRKY transcription factor family identified from Gossypium barbadense, is involved in resistance to Verticillium wilt Biotechnology & Biotechnological Equipment gbwrky1 wrky transcription factors cotton verticillium wilt disease resistance |
| title | GbWRKY1, a member of the WRKY transcription factor family identified from Gossypium barbadense, is involved in resistance to Verticillium wilt |
| title_full | GbWRKY1, a member of the WRKY transcription factor family identified from Gossypium barbadense, is involved in resistance to Verticillium wilt |
| title_fullStr | GbWRKY1, a member of the WRKY transcription factor family identified from Gossypium barbadense, is involved in resistance to Verticillium wilt |
| title_full_unstemmed | GbWRKY1, a member of the WRKY transcription factor family identified from Gossypium barbadense, is involved in resistance to Verticillium wilt |
| title_short | GbWRKY1, a member of the WRKY transcription factor family identified from Gossypium barbadense, is involved in resistance to Verticillium wilt |
| title_sort | gbwrky1 a member of the wrky transcription factor family identified from gossypium barbadense is involved in resistance to verticillium wilt |
| topic | gbwrky1 wrky transcription factors cotton verticillium wilt disease resistance |
| url | http://dx.doi.org/10.1080/13102818.2019.1667873 |
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