Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study
BackgroundHistology remains the gold-standard to assess human brain biology, so ex vivo studies using tissue from brain banks are standard practice in neuroscientific research. However, a larger number of specimens could be obtained from gross anatomy laboratories. These specimens are fixed with sol...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-10-01
|
| Series: | Frontiers in Neuroanatomy |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnana.2022.957358/full |
| _version_ | 1797995789543276544 |
|---|---|
| author | Eve-Marie Frigon Mahsa Dadar Denis Boire Josefina Maranzano Josefina Maranzano |
| author_facet | Eve-Marie Frigon Mahsa Dadar Denis Boire Josefina Maranzano Josefina Maranzano |
| author_sort | Eve-Marie Frigon |
| collection | DOAJ |
| description | BackgroundHistology remains the gold-standard to assess human brain biology, so ex vivo studies using tissue from brain banks are standard practice in neuroscientific research. However, a larger number of specimens could be obtained from gross anatomy laboratories. These specimens are fixed with solutions appropriate for dissections, but whether they also preserve brain tissue antigenicity is unclear. Therefore, we perfused mice brains with solutions used for human body preservation to assess and compare the tissue quality and antigenicity of the main cell populations.Materials and methodsTwenty-eight C57BL/6J mice were perfused with 4% formaldehyde (FAS, N = 9), salt-saturated solution (SSS, N = 9), and alcohol solution (AS, N = 10). The brains were cut into 40 μm sections for antigenicity analysis and were assessed by immunohistochemistry of four antigens: neuronal nuclei (NeuN), glial fibrillary acidic protein (GFAP astrocytes), ionized calcium-binding adaptor molecule 1 (Iba1-microglia), and myelin proteolipid protein (PLP). We compared the fixatives according to multiple variables: perfusion quality, ease of manipulation, tissue quality, immunohistochemistry quality, and antigenicity preservation.ResultsThe perfusion quality was better using FAS and worse using AS. The manipulation was very poor in SSS brains. FAS- and AS-fixed brains showed higher tissue and immunohistochemistry quality than the SSS brains. All antigens were readily observed in every specimen, regardless of the fixative solution.ConclusionSolutions designed to preserve specimens for human gross anatomy dissections also preserve tissue antigenicity in different brain cells. This offers opportunities for the use of human brains fixed in gross anatomy laboratories to assess normal or pathological conditions. |
| first_indexed | 2024-04-11T10:07:26Z |
| format | Article |
| id | doaj.art-7fe4727cd99c4330ae99390cf4c80081 |
| institution | Directory Open Access Journal |
| issn | 1662-5129 |
| language | English |
| last_indexed | 2024-04-11T10:07:26Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroanatomy |
| spelling | doaj.art-7fe4727cd99c4330ae99390cf4c800812022-12-22T04:30:12ZengFrontiers Media S.A.Frontiers in Neuroanatomy1662-51292022-10-011610.3389/fnana.2022.957358957358Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry studyEve-Marie Frigon0Mahsa Dadar1Denis Boire2Josefina Maranzano3Josefina Maranzano4Department of Anatomy, University of Quebec in Trois-Rivières, Trois-Rivières, QC, CanadaDepartment of Psychiatry, The Douglas Research Centre, McGill University, Montreal, QC, CanadaDepartment of Anatomy, University of Quebec in Trois-Rivières, Trois-Rivières, QC, CanadaDepartment of Anatomy, University of Quebec in Trois-Rivières, Trois-Rivières, QC, CanadaDepartment of Neurology and Neurosurgery, McConnell Brain Imaging Centre, Montreal Neurological Institute, McGill University, Montreal, QC, CanadaBackgroundHistology remains the gold-standard to assess human brain biology, so ex vivo studies using tissue from brain banks are standard practice in neuroscientific research. However, a larger number of specimens could be obtained from gross anatomy laboratories. These specimens are fixed with solutions appropriate for dissections, but whether they also preserve brain tissue antigenicity is unclear. Therefore, we perfused mice brains with solutions used for human body preservation to assess and compare the tissue quality and antigenicity of the main cell populations.Materials and methodsTwenty-eight C57BL/6J mice were perfused with 4% formaldehyde (FAS, N = 9), salt-saturated solution (SSS, N = 9), and alcohol solution (AS, N = 10). The brains were cut into 40 μm sections for antigenicity analysis and were assessed by immunohistochemistry of four antigens: neuronal nuclei (NeuN), glial fibrillary acidic protein (GFAP astrocytes), ionized calcium-binding adaptor molecule 1 (Iba1-microglia), and myelin proteolipid protein (PLP). We compared the fixatives according to multiple variables: perfusion quality, ease of manipulation, tissue quality, immunohistochemistry quality, and antigenicity preservation.ResultsThe perfusion quality was better using FAS and worse using AS. The manipulation was very poor in SSS brains. FAS- and AS-fixed brains showed higher tissue and immunohistochemistry quality than the SSS brains. All antigens were readily observed in every specimen, regardless of the fixative solution.ConclusionSolutions designed to preserve specimens for human gross anatomy dissections also preserve tissue antigenicity in different brain cells. This offers opportunities for the use of human brains fixed in gross anatomy laboratories to assess normal or pathological conditions.https://www.frontiersin.org/articles/10.3389/fnana.2022.957358/fullantigenicityfixationhistologyimmunohistochemistryhuman gross anatomymouse brain |
| spellingShingle | Eve-Marie Frigon Mahsa Dadar Denis Boire Josefina Maranzano Josefina Maranzano Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study Frontiers in Neuroanatomy antigenicity fixation histology immunohistochemistry human gross anatomy mouse brain |
| title | Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study |
| title_full | Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study |
| title_fullStr | Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study |
| title_full_unstemmed | Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study |
| title_short | Antigenicity is preserved with fixative solutions used in human gross anatomy: A mice brain immunohistochemistry study |
| title_sort | antigenicity is preserved with fixative solutions used in human gross anatomy a mice brain immunohistochemistry study |
| topic | antigenicity fixation histology immunohistochemistry human gross anatomy mouse brain |
| url | https://www.frontiersin.org/articles/10.3389/fnana.2022.957358/full |
| work_keys_str_mv | AT evemariefrigon antigenicityispreservedwithfixativesolutionsusedinhumangrossanatomyamicebrainimmunohistochemistrystudy AT mahsadadar antigenicityispreservedwithfixativesolutionsusedinhumangrossanatomyamicebrainimmunohistochemistrystudy AT denisboire antigenicityispreservedwithfixativesolutionsusedinhumangrossanatomyamicebrainimmunohistochemistrystudy AT josefinamaranzano antigenicityispreservedwithfixativesolutionsusedinhumangrossanatomyamicebrainimmunohistochemistrystudy AT josefinamaranzano antigenicityispreservedwithfixativesolutionsusedinhumangrossanatomyamicebrainimmunohistochemistrystudy |