A Novel Approach for the Manufacturing of Gelatin-Methacryloyl
Gelatin and its derivatives contain cell adhesion moieties as well as sites that enable proteolytic degradation, thus allowing cellular proliferation and migration. The processing of gelatin to its derivatives and/or gelatin-containing products is challenged by its gelation below 30 <inline-formu...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/14/24/5424 |
| _version_ | 1797455594457661440 |
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| author | David Grijalva Garces Carsten Philipp Radtke Jürgen Hubbuch |
| author_facet | David Grijalva Garces Carsten Philipp Radtke Jürgen Hubbuch |
| author_sort | David Grijalva Garces |
| collection | DOAJ |
| description | Gelatin and its derivatives contain cell adhesion moieties as well as sites that enable proteolytic degradation, thus allowing cellular proliferation and migration. The processing of gelatin to its derivatives and/or gelatin-containing products is challenged by its gelation below 30 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msup><mrow></mrow><mo>∘</mo></msup><mi mathvariant="normal">C</mi></mrow></semantics></math></inline-formula>. In this study, a novel strategy was developed for the dissolution and subsequent modification of gelatin to its derivative gelatin-methacryloyl (GelMA). This approach was based on the presence of urea in the buffer media, which enabled the processing at room temperature, i.e., lower than the sol–gel transition point of the gelatin solutions. The degree of functionalization was controlled by the ratio of reactant volume to the gelatin concentration. Hydrogels with tailored mechanical properties were produced by variations of the GelMA concentration and its degree of functionalization. Moreover, the biocompatibility of hydrogels was assessed and compared to hydrogels formulated with GelMA produced by the conventional method. NIH 3T3 fibroblasts were seeded onto hydrogels and the viability showed no difference from the control after a three-day incubation period. |
| first_indexed | 2024-03-09T15:56:34Z |
| format | Article |
| id | doaj.art-7fe4f914983d4403a232f4a14f1cbae4 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-09T15:56:34Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-7fe4f914983d4403a232f4a14f1cbae42023-11-24T17:31:53ZengMDPI AGPolymers2073-43602022-12-011424542410.3390/polym14245424A Novel Approach for the Manufacturing of Gelatin-MethacryloylDavid Grijalva Garces0Carsten Philipp Radtke1Jürgen Hubbuch2Institute of Functional Interfaces, Karlsruhe Institute of Technology, 76344 Eggenstein-Leopoldshafen, GermanyInstitute of Process Engineering in Life Sciences Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology, 76131 Karlsruhe, GermanyInstitute of Functional Interfaces, Karlsruhe Institute of Technology, 76344 Eggenstein-Leopoldshafen, GermanyGelatin and its derivatives contain cell adhesion moieties as well as sites that enable proteolytic degradation, thus allowing cellular proliferation and migration. The processing of gelatin to its derivatives and/or gelatin-containing products is challenged by its gelation below 30 <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msup><mrow></mrow><mo>∘</mo></msup><mi mathvariant="normal">C</mi></mrow></semantics></math></inline-formula>. In this study, a novel strategy was developed for the dissolution and subsequent modification of gelatin to its derivative gelatin-methacryloyl (GelMA). This approach was based on the presence of urea in the buffer media, which enabled the processing at room temperature, i.e., lower than the sol–gel transition point of the gelatin solutions. The degree of functionalization was controlled by the ratio of reactant volume to the gelatin concentration. Hydrogels with tailored mechanical properties were produced by variations of the GelMA concentration and its degree of functionalization. Moreover, the biocompatibility of hydrogels was assessed and compared to hydrogels formulated with GelMA produced by the conventional method. NIH 3T3 fibroblasts were seeded onto hydrogels and the viability showed no difference from the control after a three-day incubation period.https://www.mdpi.com/2073-4360/14/24/5424biomaterialscell culturefibroblastsgelatinGelMAhydrogel |
| spellingShingle | David Grijalva Garces Carsten Philipp Radtke Jürgen Hubbuch A Novel Approach for the Manufacturing of Gelatin-Methacryloyl Polymers biomaterials cell culture fibroblasts gelatin GelMA hydrogel |
| title | A Novel Approach for the Manufacturing of Gelatin-Methacryloyl |
| title_full | A Novel Approach for the Manufacturing of Gelatin-Methacryloyl |
| title_fullStr | A Novel Approach for the Manufacturing of Gelatin-Methacryloyl |
| title_full_unstemmed | A Novel Approach for the Manufacturing of Gelatin-Methacryloyl |
| title_short | A Novel Approach for the Manufacturing of Gelatin-Methacryloyl |
| title_sort | novel approach for the manufacturing of gelatin methacryloyl |
| topic | biomaterials cell culture fibroblasts gelatin GelMA hydrogel |
| url | https://www.mdpi.com/2073-4360/14/24/5424 |
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