The b-ZIP transcription factor, FgBzip16, is essential for fungal development, ascospore discharge, and pathogenicity by modulating fatty acid metabolism in Fusarium graminearum
Abstract Fusarium graminearum is an economically devastating pathogen that causes cereal worldwide. The plant disease cycle involves sexual reproduction, with the perithecium playing a crucial role in overwintering and the discharge of ascospores. Although fatty acid biosynthesis and metabolism are...
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | Phytopathology Research |
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| Online Access: | https://doi.org/10.1186/s42483-023-00190-0 |
| _version_ | 1797453696203751424 |
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| author | Bing Li Yuhe Shen Yupan Zhu Lingling Yang Ruonan Ma Hao Sun Jingang Liang Kangkang Chen Zhen Jiao Wenchao Yang |
| author_facet | Bing Li Yuhe Shen Yupan Zhu Lingling Yang Ruonan Ma Hao Sun Jingang Liang Kangkang Chen Zhen Jiao Wenchao Yang |
| author_sort | Bing Li |
| collection | DOAJ |
| description | Abstract Fusarium graminearum is an economically devastating pathogen that causes cereal worldwide. The plant disease cycle involves sexual reproduction, with the perithecium playing a crucial role in overwintering and the discharge of ascospores. Although fatty acid biosynthesis and metabolism are linked to perithecium formation and ascospore discharge, the regulation of these processes remains largely unknown. In this study, we identified and characterized FgBZIP16, as a basic leucine zipper (b-ZIP) transcription factor, in F. graminearum. Targeted gene deletion revealed that FgBzip16 is important for vegetative growth, asexual/sexual development, and plant infection. Cytological observations revealed that FgBzip16 was localized in the nucleus during the hyphal and conidial stages. FgBzip16 is essential for ascospore discharge, with transcriptomics and molecular biology showing it binds to the promoter of its target genes FGSG_05321 and FGSG_03244, which regulate ascospore discharge by encoding fatty acid synthase subunit alpha-reductase and enoyl hydratase, respectively. Altogether, these results constitute the first report of the specific functions associated with b-ZIP transcription factor FgBzip16, linking its regulatory roles to fungal development, fatty acid accumulation, and metabolism, host penetration, and pathogenicity of F. graminearum. |
| first_indexed | 2024-03-09T15:26:23Z |
| format | Article |
| id | doaj.art-7fe5616edbe2488b811569221b4da3ae |
| institution | Directory Open Access Journal |
| issn | 2524-4167 |
| language | English |
| last_indexed | 2024-03-09T15:26:23Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Phytopathology Research |
| spelling | doaj.art-7fe5616edbe2488b811569221b4da3ae2023-11-26T12:29:55ZengBMCPhytopathology Research2524-41672023-08-015111410.1186/s42483-023-00190-0The b-ZIP transcription factor, FgBzip16, is essential for fungal development, ascospore discharge, and pathogenicity by modulating fatty acid metabolism in Fusarium graminearumBing Li0Yuhe Shen1Yupan Zhu2Lingling Yang3Ruonan Ma4Hao Sun5Jingang Liang6Kangkang Chen7Zhen Jiao8Wenchao Yang9School of Plant Protection, Yangzhou UniversitySchool of Agricultural Sciences, Zhengzhou UniversitySchool of Agricultural Sciences, Zhengzhou UniversitySchool of Agricultural Sciences, Zhengzhou UniversitySchool of Agricultural Sciences, Zhengzhou UniversitySchool of Agricultural Sciences, Zhengzhou UniversityDevelopment Center of Science and Technology, Ministry of Agriculture and Rural AffairsSchool of Plant Protection, Yangzhou UniversitySchool of Agricultural Sciences, Zhengzhou UniversitySchool of Plant Protection, Yangzhou UniversityAbstract Fusarium graminearum is an economically devastating pathogen that causes cereal worldwide. The plant disease cycle involves sexual reproduction, with the perithecium playing a crucial role in overwintering and the discharge of ascospores. Although fatty acid biosynthesis and metabolism are linked to perithecium formation and ascospore discharge, the regulation of these processes remains largely unknown. In this study, we identified and characterized FgBZIP16, as a basic leucine zipper (b-ZIP) transcription factor, in F. graminearum. Targeted gene deletion revealed that FgBzip16 is important for vegetative growth, asexual/sexual development, and plant infection. Cytological observations revealed that FgBzip16 was localized in the nucleus during the hyphal and conidial stages. FgBzip16 is essential for ascospore discharge, with transcriptomics and molecular biology showing it binds to the promoter of its target genes FGSG_05321 and FGSG_03244, which regulate ascospore discharge by encoding fatty acid synthase subunit alpha-reductase and enoyl hydratase, respectively. Altogether, these results constitute the first report of the specific functions associated with b-ZIP transcription factor FgBzip16, linking its regulatory roles to fungal development, fatty acid accumulation, and metabolism, host penetration, and pathogenicity of F. graminearum.https://doi.org/10.1186/s42483-023-00190-0b-ZIP transcription factor FgBzip16DevelopmentAscospore dischargeFatty acid metabolismPathogenicity |
| spellingShingle | Bing Li Yuhe Shen Yupan Zhu Lingling Yang Ruonan Ma Hao Sun Jingang Liang Kangkang Chen Zhen Jiao Wenchao Yang The b-ZIP transcription factor, FgBzip16, is essential for fungal development, ascospore discharge, and pathogenicity by modulating fatty acid metabolism in Fusarium graminearum Phytopathology Research b-ZIP transcription factor FgBzip16 Development Ascospore discharge Fatty acid metabolism Pathogenicity |
| title | The b-ZIP transcription factor, FgBzip16, is essential for fungal development, ascospore discharge, and pathogenicity by modulating fatty acid metabolism in Fusarium graminearum |
| title_full | The b-ZIP transcription factor, FgBzip16, is essential for fungal development, ascospore discharge, and pathogenicity by modulating fatty acid metabolism in Fusarium graminearum |
| title_fullStr | The b-ZIP transcription factor, FgBzip16, is essential for fungal development, ascospore discharge, and pathogenicity by modulating fatty acid metabolism in Fusarium graminearum |
| title_full_unstemmed | The b-ZIP transcription factor, FgBzip16, is essential for fungal development, ascospore discharge, and pathogenicity by modulating fatty acid metabolism in Fusarium graminearum |
| title_short | The b-ZIP transcription factor, FgBzip16, is essential for fungal development, ascospore discharge, and pathogenicity by modulating fatty acid metabolism in Fusarium graminearum |
| title_sort | b zip transcription factor fgbzip16 is essential for fungal development ascospore discharge and pathogenicity by modulating fatty acid metabolism in fusarium graminearum |
| topic | b-ZIP transcription factor FgBzip16 Development Ascospore discharge Fatty acid metabolism Pathogenicity |
| url | https://doi.org/10.1186/s42483-023-00190-0 |
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