Morphological and Molecular Characterization of Punctodera stonei Brzeski, 1998 (Nematoda: Heteroderidae) from Virginia, USA
In August of 2021, several cysts with juveniles and eggs were discovered during a vegetation survey conducted at the Arlington National Cemetery, Virginia. Eight soil samples were collected from the rhizosphere region of the common grass (Festuca arundinacea L.) and processed at the Mycology and Nem...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2022-04-01
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| Series: | Journal of Nematology |
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| Online Access: | https://doi.org/10.2478/jofnem-2022-0008 |
| _version_ | 1827596053794258944 |
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| author | Kantor Mihail R. Subbotin Sergei A. Huse Gregory Handoo Zafar A. |
| author_facet | Kantor Mihail R. Subbotin Sergei A. Huse Gregory Handoo Zafar A. |
| author_sort | Kantor Mihail R. |
| collection | DOAJ |
| description | In August of 2021, several cysts with juveniles and eggs were discovered during a vegetation survey conducted at the Arlington National Cemetery, Virginia. Eight soil samples were collected from the rhizosphere region of the common grass (Festuca arundinacea L.) and processed at the Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL). Cysts were light to dark brown in color, and oval to pear-shaped without bullae in young cysts but present in older cysts and with prominent vulval cone. The juveniles had slightly concave stylet knobs projecting sometimes anteriorly, tail tapering gradually to a narrowly rounded terminus, and hyaline tail terminus conspicuous at least twice the length of stylet. The molecular analysis included the analysis of three gene sequence fragments: D2–D3 of 28S rRNA, ITS rRNA, and COI. The nematode species was identified by both morphological and molecular means as Stone's cyst nematode, Punctodera stonei. Detection of P. stonei in Virginia represents a new record of this species in the United States, and a second report after Canada in North America. |
| first_indexed | 2024-03-09T03:05:03Z |
| format | Article |
| id | doaj.art-7fe74a998c4946f6a6736ead9e37cf2d |
| institution | Directory Open Access Journal |
| issn | 2640-396X |
| language | English |
| last_indexed | 2024-03-09T03:05:03Z |
| publishDate | 2022-04-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Journal of Nematology |
| spelling | doaj.art-7fe74a998c4946f6a6736ead9e37cf2d2023-12-04T08:04:12ZengSciendoJournal of Nematology2640-396X2022-04-0154139720510.2478/jofnem-2022-0008Morphological and Molecular Characterization of Punctodera stonei Brzeski, 1998 (Nematoda: Heteroderidae) from Virginia, USAKantor Mihail R.0Subbotin Sergei A.1Huse Gregory2Handoo Zafar A.3Mycology and Nematology Genetic Diversity and Biology Laboratory, USDA, ARS, Northeast Area, Beltsville, MD20705, USACalifornia Department of Food and Agriculture, Plant Pest Diagnostic Center, 3294 Meadowview Road, Sacramento, CA95832, USAArlington National Cemetery, 1 Memorial AvenueArlington, VA22211, USAMycology and Nematology Genetic Diversity and Biology Laboratory, USDA, ARS, Northeast Area, Beltsville, MD20705, USAIn August of 2021, several cysts with juveniles and eggs were discovered during a vegetation survey conducted at the Arlington National Cemetery, Virginia. Eight soil samples were collected from the rhizosphere region of the common grass (Festuca arundinacea L.) and processed at the Mycology and Nematology Genetic Diversity and Biology Laboratory (MNGDBL). Cysts were light to dark brown in color, and oval to pear-shaped without bullae in young cysts but present in older cysts and with prominent vulval cone. The juveniles had slightly concave stylet knobs projecting sometimes anteriorly, tail tapering gradually to a narrowly rounded terminus, and hyaline tail terminus conspicuous at least twice the length of stylet. The molecular analysis included the analysis of three gene sequence fragments: D2–D3 of 28S rRNA, ITS rRNA, and COI. The nematode species was identified by both morphological and molecular means as Stone's cyst nematode, Punctodera stonei. Detection of P. stonei in Virginia represents a new record of this species in the United States, and a second report after Canada in North America.https://doi.org/10.2478/jofnem-2022-0008cyst nematodegrasspunctodera stoneivirginiaunited statesdetectiondiagnosissystematicstaxonomy |
| spellingShingle | Kantor Mihail R. Subbotin Sergei A. Huse Gregory Handoo Zafar A. Morphological and Molecular Characterization of Punctodera stonei Brzeski, 1998 (Nematoda: Heteroderidae) from Virginia, USA Journal of Nematology cyst nematode grass punctodera stonei virginia united states detection diagnosis systematics taxonomy |
| title | Morphological and Molecular Characterization of Punctodera stonei Brzeski, 1998 (Nematoda: Heteroderidae) from Virginia, USA |
| title_full | Morphological and Molecular Characterization of Punctodera stonei Brzeski, 1998 (Nematoda: Heteroderidae) from Virginia, USA |
| title_fullStr | Morphological and Molecular Characterization of Punctodera stonei Brzeski, 1998 (Nematoda: Heteroderidae) from Virginia, USA |
| title_full_unstemmed | Morphological and Molecular Characterization of Punctodera stonei Brzeski, 1998 (Nematoda: Heteroderidae) from Virginia, USA |
| title_short | Morphological and Molecular Characterization of Punctodera stonei Brzeski, 1998 (Nematoda: Heteroderidae) from Virginia, USA |
| title_sort | morphological and molecular characterization of punctodera stonei brzeski 1998 nematoda heteroderidae from virginia usa |
| topic | cyst nematode grass punctodera stonei virginia united states detection diagnosis systematics taxonomy |
| url | https://doi.org/10.2478/jofnem-2022-0008 |
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