Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated.
Trypanosoma brucei is a protozoan parasite that is used as a model organism to study such biological phenomena as gene expression, protein trafficking, and cytoskeletal biogenesis. In T. brucei, endocytosis and exocytosis occur exclusively through a sequestered organelle called the flagellar pocket...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2008-05-01
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| Series: | PLoS Biology |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18462016/?tool=EBI |
| _version_ | 1818567320683937792 |
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| author | Mélanie Bonhivers Sophie Nowacki Nicolas Landrein Derrick R Robinson |
| author_facet | Mélanie Bonhivers Sophie Nowacki Nicolas Landrein Derrick R Robinson |
| author_sort | Mélanie Bonhivers |
| collection | DOAJ |
| description | Trypanosoma brucei is a protozoan parasite that is used as a model organism to study such biological phenomena as gene expression, protein trafficking, and cytoskeletal biogenesis. In T. brucei, endocytosis and exocytosis occur exclusively through a sequestered organelle called the flagellar pocket (FP), an invagination of the pellicular membrane. The pocket is the sole site for specific receptors thus maintaining them inaccessible to components of the innate immune system of the mammalian host. The FP is also responsible for the sorting of protective parasite glycoproteins targeted to, or recycling from, the pellicular membrane, and for the removal of host antibodies from the cell surface. Here, we describe the first characterisation of a flagellar pocket cytoskeletal protein, BILBO1. BILBO1 functions to form a cytoskeleton framework upon which the FP is made and which is also required and essential for FP biogenesis and cell survival. Remarkably, RNA interference (RNAi)-mediated ablation of BILBO1 in insect procyclic-form parasites prevents FP biogenesis and induces vesicle accumulation, Golgi swelling, the aberrant repositioning of the new flagellum, and cell death. Cultured bloodstream-form parasites are also nonviable when subjected to BILBO1 RNAi. These results provide the first molecular evidence for cytoskeletally mediated FP biogenesis. |
| first_indexed | 2024-12-14T06:21:53Z |
| format | Article |
| id | doaj.art-7fe882aa5dda45fcbac74bdf134a32eb |
| institution | Directory Open Access Journal |
| issn | 1544-9173 1545-7885 |
| language | English |
| last_indexed | 2024-12-14T06:21:53Z |
| publishDate | 2008-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj.art-7fe882aa5dda45fcbac74bdf134a32eb2022-12-21T23:13:48ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852008-05-0165e10510.1371/journal.pbio.0060105Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated.Mélanie BonhiversSophie NowackiNicolas LandreinDerrick R RobinsonTrypanosoma brucei is a protozoan parasite that is used as a model organism to study such biological phenomena as gene expression, protein trafficking, and cytoskeletal biogenesis. In T. brucei, endocytosis and exocytosis occur exclusively through a sequestered organelle called the flagellar pocket (FP), an invagination of the pellicular membrane. The pocket is the sole site for specific receptors thus maintaining them inaccessible to components of the innate immune system of the mammalian host. The FP is also responsible for the sorting of protective parasite glycoproteins targeted to, or recycling from, the pellicular membrane, and for the removal of host antibodies from the cell surface. Here, we describe the first characterisation of a flagellar pocket cytoskeletal protein, BILBO1. BILBO1 functions to form a cytoskeleton framework upon which the FP is made and which is also required and essential for FP biogenesis and cell survival. Remarkably, RNA interference (RNAi)-mediated ablation of BILBO1 in insect procyclic-form parasites prevents FP biogenesis and induces vesicle accumulation, Golgi swelling, the aberrant repositioning of the new flagellum, and cell death. Cultured bloodstream-form parasites are also nonviable when subjected to BILBO1 RNAi. These results provide the first molecular evidence for cytoskeletally mediated FP biogenesis.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18462016/?tool=EBI |
| spellingShingle | Mélanie Bonhivers Sophie Nowacki Nicolas Landrein Derrick R Robinson Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated. PLoS Biology |
| title | Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated. |
| title_full | Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated. |
| title_fullStr | Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated. |
| title_full_unstemmed | Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated. |
| title_short | Biogenesis of the trypanosome endo-exocytotic organelle is cytoskeleton mediated. |
| title_sort | biogenesis of the trypanosome endo exocytotic organelle is cytoskeleton mediated |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18462016/?tool=EBI |
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