Morphofunctional State and Circadian Rhythms of the Liver of Female Rats under the Influence of Chronic Alcohol Intoxication and Constant Lighting
A separate and combined effect of constant illumination and chronic alcohol intoxication (CAI) on diurnal dynamics of micromorphometric parameters of hepatocytes in female Wistar rats and <i>p53</i>, <i>Ki-67</i>, <i>PER2</i>, <i>BMAL1,</i> and <i&g...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/18/10744 |
| Summary: | A separate and combined effect of constant illumination and chronic alcohol intoxication (CAI) on diurnal dynamics of micromorphometric parameters of hepatocytes in female Wistar rats and <i>p53</i>, <i>Ki-67</i>, <i>PER2</i>, <i>BMAL1,</i> and <i>ADH5</i> expression in these cells were studied. The increase in apoptotic activity and proliferation in all animals under the action of chronodestructors is shown. All experimental animals showed a decrease in <i>BMAL1</i> expression and increase in <i>PER2</i> expression; <i>ADH5</i> is overexpressed under the influence of ethanol. Circadian rhythms (CRs) of <i>BMAL1</i>, <i>PER2</i>, <i>p53</i>, and <i>Ki-67</i> expression persist in all groups, except combined action of chronodestructors, and <i>ADH5</i> CRs persist in all groups—thus, these rhythms in females are quite stable. CRs of the hepatocyte nuclei area are preserved in all the studied groups, although they undergo a significant shift. At the same time, the CRs of the hepatocyte area are destroyed under the action of light, both independently and in combination with CAI, and the CR of the nuclear-cytoplasmic ratio (NCR) is destroyed by exposure to CAI. It can be assumed that CRs of the hepatocyte area are significantly affected by dark deprivation and NCR rhythm is sensitive to ethanol consumption, while the stability of studied genes’ expression rhythms at separate influences of studied chronodestructors is maintained by yet unknown adaptation mechanisms. It is necessary to note that, according to our previous studies of male rats, rat females show significantly greater stability of the studied CRs. |
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| ISSN: | 1661-6596 1422-0067 |