Transgene and Chemical Transdifferentiation of Somatic Cells for Rapid and Efficient Neurological Disease Cell Models
For neurological diseases, molecular and cellular research relies on the use of model systems to investigate disease processes and test potential therapeutics. The last decade has witnessed an increase in the number of studies using induced pluripotent stem cells to generate disease relevant cell ty...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-05-01
|
| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fncel.2022.858432/full |
| _version_ | 1828845000509620224 |
|---|---|
| author | Neville Ng Neville Ng Michelle Newbery Michelle Newbery Simon Maksour Simon Maksour Mirella Dottori Mirella Dottori Ronald Sluyter Ronald Sluyter Lezanne Ooi Lezanne Ooi |
| author_facet | Neville Ng Neville Ng Michelle Newbery Michelle Newbery Simon Maksour Simon Maksour Mirella Dottori Mirella Dottori Ronald Sluyter Ronald Sluyter Lezanne Ooi Lezanne Ooi |
| author_sort | Neville Ng |
| collection | DOAJ |
| description | For neurological diseases, molecular and cellular research relies on the use of model systems to investigate disease processes and test potential therapeutics. The last decade has witnessed an increase in the number of studies using induced pluripotent stem cells to generate disease relevant cell types from patients. The reprogramming process permits the generation of a large number of cells but is potentially disadvantaged by introducing variability in clonal lines and the removal of phenotypes of aging, which are critical to understand neurodegenerative diseases. An under-utilized approach to disease modeling involves the transdifferentiation of aged cells from patients, such as fibroblasts or blood cells, into various neural cell types. In this review we discuss techniques used for rapid and efficient direct conversion to neural cell types. We examine the limitations and future perspectives of this rapidly advancing field that could improve neurological disease modeling and drug discovery. |
| first_indexed | 2024-12-12T21:18:38Z |
| format | Article |
| id | doaj.art-7fed7f86118e48ef8a9db8587ecd71e3 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-12T21:18:38Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-7fed7f86118e48ef8a9db8587ecd71e32022-12-22T00:11:40ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022022-05-011610.3389/fncel.2022.858432858432Transgene and Chemical Transdifferentiation of Somatic Cells for Rapid and Efficient Neurological Disease Cell ModelsNeville Ng0Neville Ng1Michelle Newbery2Michelle Newbery3Simon Maksour4Simon Maksour5Mirella Dottori6Mirella Dottori7Ronald Sluyter8Ronald Sluyter9Lezanne Ooi10Lezanne Ooi11Illawarra Health and Medical Research Institute, Wollongong, NSW, AustraliaSchool of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, NSW, AustraliaIllawarra Health and Medical Research Institute, Wollongong, NSW, AustraliaSchool of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, NSW, AustraliaIllawarra Health and Medical Research Institute, Wollongong, NSW, AustraliaSchool of Medical, Indigenous and Health Sciences, University of Wollongong, Wollongong, NSW, AustraliaIllawarra Health and Medical Research Institute, Wollongong, NSW, AustraliaSchool of Medical, Indigenous and Health Sciences, University of Wollongong, Wollongong, NSW, AustraliaIllawarra Health and Medical Research Institute, Wollongong, NSW, AustraliaSchool of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, NSW, AustraliaIllawarra Health and Medical Research Institute, Wollongong, NSW, AustraliaSchool of Chemistry and Molecular Bioscience and Molecular Horizons, University of Wollongong, Wollongong, NSW, AustraliaFor neurological diseases, molecular and cellular research relies on the use of model systems to investigate disease processes and test potential therapeutics. The last decade has witnessed an increase in the number of studies using induced pluripotent stem cells to generate disease relevant cell types from patients. The reprogramming process permits the generation of a large number of cells but is potentially disadvantaged by introducing variability in clonal lines and the removal of phenotypes of aging, which are critical to understand neurodegenerative diseases. An under-utilized approach to disease modeling involves the transdifferentiation of aged cells from patients, such as fibroblasts or blood cells, into various neural cell types. In this review we discuss techniques used for rapid and efficient direct conversion to neural cell types. We examine the limitations and future perspectives of this rapidly advancing field that could improve neurological disease modeling and drug discovery.https://www.frontiersin.org/articles/10.3389/fncel.2022.858432/fulltransdifferentiationreprogrammingdifferentiationneurodegenerationtranscription factorsaging |
| spellingShingle | Neville Ng Neville Ng Michelle Newbery Michelle Newbery Simon Maksour Simon Maksour Mirella Dottori Mirella Dottori Ronald Sluyter Ronald Sluyter Lezanne Ooi Lezanne Ooi Transgene and Chemical Transdifferentiation of Somatic Cells for Rapid and Efficient Neurological Disease Cell Models Frontiers in Cellular Neuroscience transdifferentiation reprogramming differentiation neurodegeneration transcription factors aging |
| title | Transgene and Chemical Transdifferentiation of Somatic Cells for Rapid and Efficient Neurological Disease Cell Models |
| title_full | Transgene and Chemical Transdifferentiation of Somatic Cells for Rapid and Efficient Neurological Disease Cell Models |
| title_fullStr | Transgene and Chemical Transdifferentiation of Somatic Cells for Rapid and Efficient Neurological Disease Cell Models |
| title_full_unstemmed | Transgene and Chemical Transdifferentiation of Somatic Cells for Rapid and Efficient Neurological Disease Cell Models |
| title_short | Transgene and Chemical Transdifferentiation of Somatic Cells for Rapid and Efficient Neurological Disease Cell Models |
| title_sort | transgene and chemical transdifferentiation of somatic cells for rapid and efficient neurological disease cell models |
| topic | transdifferentiation reprogramming differentiation neurodegeneration transcription factors aging |
| url | https://www.frontiersin.org/articles/10.3389/fncel.2022.858432/full |
| work_keys_str_mv | AT nevilleng transgeneandchemicaltransdifferentiationofsomaticcellsforrapidandefficientneurologicaldiseasecellmodels AT nevilleng transgeneandchemicaltransdifferentiationofsomaticcellsforrapidandefficientneurologicaldiseasecellmodels AT michellenewbery transgeneandchemicaltransdifferentiationofsomaticcellsforrapidandefficientneurologicaldiseasecellmodels AT michellenewbery transgeneandchemicaltransdifferentiationofsomaticcellsforrapidandefficientneurologicaldiseasecellmodels AT simonmaksour transgeneandchemicaltransdifferentiationofsomaticcellsforrapidandefficientneurologicaldiseasecellmodels AT simonmaksour transgeneandchemicaltransdifferentiationofsomaticcellsforrapidandefficientneurologicaldiseasecellmodels AT mirelladottori transgeneandchemicaltransdifferentiationofsomaticcellsforrapidandefficientneurologicaldiseasecellmodels AT mirelladottori transgeneandchemicaltransdifferentiationofsomaticcellsforrapidandefficientneurologicaldiseasecellmodels AT ronaldsluyter transgeneandchemicaltransdifferentiationofsomaticcellsforrapidandefficientneurologicaldiseasecellmodels AT ronaldsluyter transgeneandchemicaltransdifferentiationofsomaticcellsforrapidandefficientneurologicaldiseasecellmodels AT lezanneooi transgeneandchemicaltransdifferentiationofsomaticcellsforrapidandefficientneurologicaldiseasecellmodels AT lezanneooi transgeneandchemicaltransdifferentiationofsomaticcellsforrapidandefficientneurologicaldiseasecellmodels |