Load regulates bone formation and Sclerostin expression through a TGFβ-dependent mechanism.
Bone continually adapts to meet changing physical and biological demands. Osteoblasts, osteoclasts, and osteocytes cooperate to integrate these physical and biochemical cues to maintain bone homeostasis. Although TGFβ acts on all three of these cell types to maintain bone homeostasis, the extent to...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3538690?pdf=render |
| _version_ | 1818288677226283008 |
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| author | Jacqueline Nguyen Simon Y Tang Daniel Nguyen Tamara Alliston |
| author_facet | Jacqueline Nguyen Simon Y Tang Daniel Nguyen Tamara Alliston |
| author_sort | Jacqueline Nguyen |
| collection | DOAJ |
| description | Bone continually adapts to meet changing physical and biological demands. Osteoblasts, osteoclasts, and osteocytes cooperate to integrate these physical and biochemical cues to maintain bone homeostasis. Although TGFβ acts on all three of these cell types to maintain bone homeostasis, the extent to which it participates in the adaptation of bone to mechanical load is unknown. Here, we investigated the role of the TGFβ pathway in load-induced bone formation and the regulation of Sclerostin, a mechanosensitive antagonist of bone anabolism. We found that mechanical load rapidly represses the net activity of the TGFβ pathway in osteocytes, resulting in reduced phosphorylation and activity of key downstream effectors, Smad2 and Smad3. Loss of TGFβ sensitivity compromises the anabolic response of bone to mechanical load, demonstrating that the mechanosensitive regulation of TGFβ signaling is essential for load-induced bone formation. Furthermore, sensitivity to TGFβ is required for the mechanosensitive regulation of Sclerostin, which is induced by TGFβ in a Smad3-dependent manner. Together, our results show that physical cues maintain bone homeostasis through the TGFβ pathway to regulate Sclerostin expression and the deposition of new bone. |
| first_indexed | 2024-12-13T02:00:11Z |
| format | Article |
| id | doaj.art-7fef5159d71a47c6ba2df220dc830e39 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T02:00:11Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7fef5159d71a47c6ba2df220dc830e392022-12-22T00:03:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0181e5381310.1371/journal.pone.0053813Load regulates bone formation and Sclerostin expression through a TGFβ-dependent mechanism.Jacqueline NguyenSimon Y TangDaniel NguyenTamara AllistonBone continually adapts to meet changing physical and biological demands. Osteoblasts, osteoclasts, and osteocytes cooperate to integrate these physical and biochemical cues to maintain bone homeostasis. Although TGFβ acts on all three of these cell types to maintain bone homeostasis, the extent to which it participates in the adaptation of bone to mechanical load is unknown. Here, we investigated the role of the TGFβ pathway in load-induced bone formation and the regulation of Sclerostin, a mechanosensitive antagonist of bone anabolism. We found that mechanical load rapidly represses the net activity of the TGFβ pathway in osteocytes, resulting in reduced phosphorylation and activity of key downstream effectors, Smad2 and Smad3. Loss of TGFβ sensitivity compromises the anabolic response of bone to mechanical load, demonstrating that the mechanosensitive regulation of TGFβ signaling is essential for load-induced bone formation. Furthermore, sensitivity to TGFβ is required for the mechanosensitive regulation of Sclerostin, which is induced by TGFβ in a Smad3-dependent manner. Together, our results show that physical cues maintain bone homeostasis through the TGFβ pathway to regulate Sclerostin expression and the deposition of new bone.http://europepmc.org/articles/PMC3538690?pdf=render |
| spellingShingle | Jacqueline Nguyen Simon Y Tang Daniel Nguyen Tamara Alliston Load regulates bone formation and Sclerostin expression through a TGFβ-dependent mechanism. PLoS ONE |
| title | Load regulates bone formation and Sclerostin expression through a TGFβ-dependent mechanism. |
| title_full | Load regulates bone formation and Sclerostin expression through a TGFβ-dependent mechanism. |
| title_fullStr | Load regulates bone formation and Sclerostin expression through a TGFβ-dependent mechanism. |
| title_full_unstemmed | Load regulates bone formation and Sclerostin expression through a TGFβ-dependent mechanism. |
| title_short | Load regulates bone formation and Sclerostin expression through a TGFβ-dependent mechanism. |
| title_sort | load regulates bone formation and sclerostin expression through a tgfβ dependent mechanism |
| url | http://europepmc.org/articles/PMC3538690?pdf=render |
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