A modified BG-Sentinel trap equipped with FTA card as a novel tool for mosquito-borne disease surveillance: a field test for flavivirus detection
Abstract Early detection of pathogens in vectors is important in preventing the spread of arboviral diseases, providing a timely indicator of pathogen circulation before outbreaks occur. However, entomological surveillance may face logistical constraints, such as maintaining the cold chain, and reso...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-39857-1 |
| _version_ | 1797560203000938496 |
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| author | Sara Manzi Luca Nelli Claudia Fortuna Francesco Severini Luciano Toma M. Di Luca Alice Michelutti Michela Bertola Francesco Gradoni Federica Toniolo Sofia Sgubin Florigio Lista Michele Pazienza Fabrizio Montarsi Marco Pombi |
| author_facet | Sara Manzi Luca Nelli Claudia Fortuna Francesco Severini Luciano Toma M. Di Luca Alice Michelutti Michela Bertola Francesco Gradoni Federica Toniolo Sofia Sgubin Florigio Lista Michele Pazienza Fabrizio Montarsi Marco Pombi |
| author_sort | Sara Manzi |
| collection | DOAJ |
| description | Abstract Early detection of pathogens in vectors is important in preventing the spread of arboviral diseases, providing a timely indicator of pathogen circulation before outbreaks occur. However, entomological surveillance may face logistical constraints, such as maintaining the cold chain, and resource limitations, such as the field and laboratory workload of mosquito processing. We propose an FTA card-based trapping system that aims to simplify both field and laboratory phases of arbovirus surveillance. We modified a BG-Sentinel trap to include a mosquito collection chamber and a sugar feeding source through an FTA card soaked in a long-lasting viscous solution of honey and hydroxy-cellulose hydrogel. The FTA card ensures environmental preservation of nucleic acids, allowing continuous collection and feeding activity of specimens for several days and reducing the effort required for viral detection. We tested the trap prototype during two field seasons (2019 and 2021) in North-eastern Italy and compared it to CDC-CO2 trapping applied in West Nile and Usutu virus regional surveillance. Collections by the BG-FTA approach detected high species diversity, including Culex pipiens, Aedes albopictus, Culex modestus, Anopheles maculipennis sensu lato and Ochlerotatus caspius. When used for two-days sampling, the BG-FTA trap performed equally to CDC also for the WNV-major vector Cx. pipiens. The FTA cards detected both WNV and USUV, confirming the reliability of this novel approach to detect viral circulation in infectious mosquitoes. We recommend this surveillance approach as a particularly useful alternative in multi-target surveillance, for sampling in remote areas and in contexts characterized by high mosquito densities and diversity. |
| first_indexed | 2024-03-10T17:57:05Z |
| format | Article |
| id | doaj.art-7ff22afbb68143a58f599be967f9f2fe |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T17:57:05Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-7ff22afbb68143a58f599be967f9f2fe2023-11-20T09:10:19ZengNature PortfolioScientific Reports2045-23222023-08-0113111210.1038/s41598-023-39857-1A modified BG-Sentinel trap equipped with FTA card as a novel tool for mosquito-borne disease surveillance: a field test for flavivirus detectionSara Manzi0Luca Nelli1Claudia Fortuna2Francesco Severini3Luciano Toma4M. Di Luca5Alice Michelutti6Michela Bertola7Francesco Gradoni8Federica Toniolo9Sofia Sgubin10Florigio Lista11Michele Pazienza12Fabrizio Montarsi13Marco Pombi14Dipartimento di Sanità Pubblica e Malattie Infettive, Sapienza Università di RomaSchool of Biodiversity, One Health and Veterinary Medicine, University of GlasgowDipartimento di Malattie Infettive, Istituto Superiore di SanitàDipartimento di Malattie Infettive, Istituto Superiore di SanitàDipartimento di Malattie Infettive, Istituto Superiore di SanitàDipartimento di Malattie Infettive, Istituto Superiore di SanitàIstituto Zooprofilattico Sperimentale Delle VenezieIstituto Zooprofilattico Sperimentale Delle VenezieIstituto Zooprofilattico Sperimentale Delle VenezieIstituto Zooprofilattico Sperimentale Delle VenezieIstituto Zooprofilattico Sperimentale Delle VenezieIstituto di Scienze Biomediche Della DifesaStato Maggiore Della DifesaIstituto Zooprofilattico Sperimentale Delle VenezieDipartimento di Sanità Pubblica e Malattie Infettive, Sapienza Università di RomaAbstract Early detection of pathogens in vectors is important in preventing the spread of arboviral diseases, providing a timely indicator of pathogen circulation before outbreaks occur. However, entomological surveillance may face logistical constraints, such as maintaining the cold chain, and resource limitations, such as the field and laboratory workload of mosquito processing. We propose an FTA card-based trapping system that aims to simplify both field and laboratory phases of arbovirus surveillance. We modified a BG-Sentinel trap to include a mosquito collection chamber and a sugar feeding source through an FTA card soaked in a long-lasting viscous solution of honey and hydroxy-cellulose hydrogel. The FTA card ensures environmental preservation of nucleic acids, allowing continuous collection and feeding activity of specimens for several days and reducing the effort required for viral detection. We tested the trap prototype during two field seasons (2019 and 2021) in North-eastern Italy and compared it to CDC-CO2 trapping applied in West Nile and Usutu virus regional surveillance. Collections by the BG-FTA approach detected high species diversity, including Culex pipiens, Aedes albopictus, Culex modestus, Anopheles maculipennis sensu lato and Ochlerotatus caspius. When used for two-days sampling, the BG-FTA trap performed equally to CDC also for the WNV-major vector Cx. pipiens. The FTA cards detected both WNV and USUV, confirming the reliability of this novel approach to detect viral circulation in infectious mosquitoes. We recommend this surveillance approach as a particularly useful alternative in multi-target surveillance, for sampling in remote areas and in contexts characterized by high mosquito densities and diversity.https://doi.org/10.1038/s41598-023-39857-1 |
| spellingShingle | Sara Manzi Luca Nelli Claudia Fortuna Francesco Severini Luciano Toma M. Di Luca Alice Michelutti Michela Bertola Francesco Gradoni Federica Toniolo Sofia Sgubin Florigio Lista Michele Pazienza Fabrizio Montarsi Marco Pombi A modified BG-Sentinel trap equipped with FTA card as a novel tool for mosquito-borne disease surveillance: a field test for flavivirus detection Scientific Reports |
| title | A modified BG-Sentinel trap equipped with FTA card as a novel tool for mosquito-borne disease surveillance: a field test for flavivirus detection |
| title_full | A modified BG-Sentinel trap equipped with FTA card as a novel tool for mosquito-borne disease surveillance: a field test for flavivirus detection |
| title_fullStr | A modified BG-Sentinel trap equipped with FTA card as a novel tool for mosquito-borne disease surveillance: a field test for flavivirus detection |
| title_full_unstemmed | A modified BG-Sentinel trap equipped with FTA card as a novel tool for mosquito-borne disease surveillance: a field test for flavivirus detection |
| title_short | A modified BG-Sentinel trap equipped with FTA card as a novel tool for mosquito-borne disease surveillance: a field test for flavivirus detection |
| title_sort | modified bg sentinel trap equipped with fta card as a novel tool for mosquito borne disease surveillance a field test for flavivirus detection |
| url | https://doi.org/10.1038/s41598-023-39857-1 |
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