LPMO as a Key Enzyme in the Sustainable Conversion of Lignocellulosic Biomass

The importance of the lytic polysaccharide monooxygenase (LPMO) enzyme in the preparation of lignocellulosic raw materials for production in biorefineries has been confirmed in numerous investigations. Therefore, LPMO enzymes were investigated to explore the enzyme-substrate interaction with the aim...

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Main Authors: Tonči Rezić, Martina Andlar, Ana Vrsalović Presečki
Format: Article
Language:English
Published: Croatian Society of Chemical Engineers 2023-05-01
Series:Kemija u Industriji
Subjects:
Online Access:http://silverstripe.fkit.hr/kui/assets/Uploads/12-389-395-KUI-5-6-2023.pdf
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author Tonči Rezić
Martina Andlar
Ana Vrsalović Presečki
author_facet Tonči Rezić
Martina Andlar
Ana Vrsalović Presečki
author_sort Tonči Rezić
collection DOAJ
description The importance of the lytic polysaccharide monooxygenase (LPMO) enzyme in the preparation of lignocellulosic raw materials for production in biorefineries has been confirmed in numerous investigations. Therefore, LPMO enzymes were investigated to explore the enzyme-substrate interaction with the aim of successful biomass conversion in biorefinery processes. After reductive activation of LPMOs active site, they cleave the substrate and prepare it for biomass degradation by hydrolytic enzymes. In this paper, the role of LPMO in lignocellulosic biomass conversion is described based on the recent studies of: LPMO enzyme structure, LPMO substrate preferences, and the LPMO reaction mechanism. These findings are important for the selection of suitable bioprocess conditions with the aim of LPMO activation/stabilisation in biorefinery production processes.
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spelling doaj.art-7ff3790448d3448593da96811699ff892023-05-16T23:38:10ZengCroatian Society of Chemical EngineersKemija u Industriji0022-98301334-90902023-05-01725-638939510.15255/KUI.2023.010LPMO as a Key Enzyme in the Sustainable Conversion of Lignocellulosic BiomassTonči Rezić0Martina Andlar1Ana Vrsalović Presečki2University of Zagreb, Faculty of Food Technology and Biotechnology, Pierottijeva 6, 10 000 Zagreb, CroatiaUniversity of Zagreb, Faculty of Food Technology and Biotechnology, Pierottijeva 6, 10 000 Zagreb, CroatiaUniversity of Zagreb, Faculty of Chemical Engineering and Technology, Trg Marka Marulića 19, 10 000 Zagreb, CroatiaThe importance of the lytic polysaccharide monooxygenase (LPMO) enzyme in the preparation of lignocellulosic raw materials for production in biorefineries has been confirmed in numerous investigations. Therefore, LPMO enzymes were investigated to explore the enzyme-substrate interaction with the aim of successful biomass conversion in biorefinery processes. After reductive activation of LPMOs active site, they cleave the substrate and prepare it for biomass degradation by hydrolytic enzymes. In this paper, the role of LPMO in lignocellulosic biomass conversion is described based on the recent studies of: LPMO enzyme structure, LPMO substrate preferences, and the LPMO reaction mechanism. These findings are important for the selection of suitable bioprocess conditions with the aim of LPMO activation/stabilisation in biorefinery production processes.http://silverstripe.fkit.hr/kui/assets/Uploads/12-389-395-KUI-5-6-2023.pdflytic polysaccharide monooxygenasesubstratelignocellulosic biomassreaction mechanismenzymatic hydrolysis
spellingShingle Tonči Rezić
Martina Andlar
Ana Vrsalović Presečki
LPMO as a Key Enzyme in the Sustainable Conversion of Lignocellulosic Biomass
Kemija u Industriji
lytic polysaccharide monooxygenase
substrate
lignocellulosic biomass
reaction mechanism
enzymatic hydrolysis
title LPMO as a Key Enzyme in the Sustainable Conversion of Lignocellulosic Biomass
title_full LPMO as a Key Enzyme in the Sustainable Conversion of Lignocellulosic Biomass
title_fullStr LPMO as a Key Enzyme in the Sustainable Conversion of Lignocellulosic Biomass
title_full_unstemmed LPMO as a Key Enzyme in the Sustainable Conversion of Lignocellulosic Biomass
title_short LPMO as a Key Enzyme in the Sustainable Conversion of Lignocellulosic Biomass
title_sort lpmo as a key enzyme in the sustainable conversion of lignocellulosic biomass
topic lytic polysaccharide monooxygenase
substrate
lignocellulosic biomass
reaction mechanism
enzymatic hydrolysis
url http://silverstripe.fkit.hr/kui/assets/Uploads/12-389-395-KUI-5-6-2023.pdf
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