Proteomics of <it>Streptococcus gordonii</it> within a model developing oral microbial community
<p>Abstract</p> <p>Background</p> <p><it>Streptococcus gordonii</it> is one of several species that can initiate the formation of oral biofilms that develop into the complex multispecies microbial communities referred to as dental plaque. It is in the contex...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-09-01
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| Series: | BMC Microbiology |
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| Online Access: | http://www.biomedcentral.com/1471-2180/12/211 |
| _version_ | 1830215407605121024 |
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| author | Hendrickson Erik L Wang Tiansong Dickinson Brittany C Whitmore Sarah E Wright Christopher J Lamont Richard J Hackett Murray |
| author_facet | Hendrickson Erik L Wang Tiansong Dickinson Brittany C Whitmore Sarah E Wright Christopher J Lamont Richard J Hackett Murray |
| author_sort | Hendrickson Erik L |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Streptococcus gordonii</it> is one of several species that can initiate the formation of oral biofilms that develop into the complex multispecies microbial communities referred to as dental plaque. It is in the context of dental plaque that periodontal pathogens such as <it>Porphyromonas gingivalis</it> cause disease. We have previously reported a whole cell quantitative proteomics investigation of <it>P. gingivalis</it> in a model dental plaque community of <it>S. gordonii</it>, <it>P. gingivalis</it>, and <it>Fusobacterium nucleatum</it>. Here we report the adaptation of <it>S. gordonii</it> to the same model.</p> <p>Results</p> <p>1122 <it>S. gordonii</it> proteins were detected in <it>S. gordonii</it> control samples, 915 in communities with <it>F. nucleatum</it>, 849 with <it>P. gingivalis</it>, and 649 with all three organisms. Quantitative comparisons showed extensive proteome changes in association with <it>F. nucleatum</it> or <it>P. gingivalis</it> individually or both <it>P. gingivalis</it> and <it>F. nucleatum</it> together. The changes were species specific, though the <it>P. gingivalis</it> interaction may be dominant, indicated by large differences between the proteomes with <it>F. nucleatum</it> or <it>P. gingivalis</it> but limited changes between communities with <it>P. gingivalis</it> or both <it>P. gingivalis</it> and <it>F. nucleatum</it>. The results were inspected manually and an ontology analysis conducted using DAVID. Extensive changes were seen in nutrition pathways with increases in energy metabolism and changes in the resulting byproducts, while the acid and sugar repressed PTS (phosphoenolpyruvate dependent phosphotransferase system) sugar transport systems showed decreases. These results were seen across all the multispecies samples, though with different profiles according to the partner species. <it>F. nucleatum</it> association decreased proteins for the metabolic end products acetate and ethanol but increased lactate, the primary source of acidity from streptococcal cultures. <it>P. gingivalis</it> containing samples had a reduction in levels of proteins for ethanol and formate but increased proteins for both acetate and lactate production. The communities also showed increases in exopolysaccharide synthesis, amino acid biosynthesis, and oxidative stress protection and decreases in adhesion and transporter proteins.</p> <p>Conclusion</p> <p>This study showed that <it>S. gordonii</it> demonstrates species specific responses during interactions with <it>F. nucleatum</it> or <it>P. gingivalis</it>. Extensive changes were seen in energy metabolism and byproduct production implicating nutrient transfer as an important community interaction.</p> |
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| issn | 1471-2180 |
| language | English |
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| spelling | doaj.art-7ff4ecaa2b6d45db97c62cdc2ee985282022-12-21T21:17:10ZengBMCBMC Microbiology1471-21802012-09-0112121110.1186/1471-2180-12-211Proteomics of <it>Streptococcus gordonii</it> within a model developing oral microbial communityHendrickson Erik LWang TiansongDickinson Brittany CWhitmore Sarah EWright Christopher JLamont Richard JHackett Murray<p>Abstract</p> <p>Background</p> <p><it>Streptococcus gordonii</it> is one of several species that can initiate the formation of oral biofilms that develop into the complex multispecies microbial communities referred to as dental plaque. It is in the context of dental plaque that periodontal pathogens such as <it>Porphyromonas gingivalis</it> cause disease. We have previously reported a whole cell quantitative proteomics investigation of <it>P. gingivalis</it> in a model dental plaque community of <it>S. gordonii</it>, <it>P. gingivalis</it>, and <it>Fusobacterium nucleatum</it>. Here we report the adaptation of <it>S. gordonii</it> to the same model.</p> <p>Results</p> <p>1122 <it>S. gordonii</it> proteins were detected in <it>S. gordonii</it> control samples, 915 in communities with <it>F. nucleatum</it>, 849 with <it>P. gingivalis</it>, and 649 with all three organisms. Quantitative comparisons showed extensive proteome changes in association with <it>F. nucleatum</it> or <it>P. gingivalis</it> individually or both <it>P. gingivalis</it> and <it>F. nucleatum</it> together. The changes were species specific, though the <it>P. gingivalis</it> interaction may be dominant, indicated by large differences between the proteomes with <it>F. nucleatum</it> or <it>P. gingivalis</it> but limited changes between communities with <it>P. gingivalis</it> or both <it>P. gingivalis</it> and <it>F. nucleatum</it>. The results were inspected manually and an ontology analysis conducted using DAVID. Extensive changes were seen in nutrition pathways with increases in energy metabolism and changes in the resulting byproducts, while the acid and sugar repressed PTS (phosphoenolpyruvate dependent phosphotransferase system) sugar transport systems showed decreases. These results were seen across all the multispecies samples, though with different profiles according to the partner species. <it>F. nucleatum</it> association decreased proteins for the metabolic end products acetate and ethanol but increased lactate, the primary source of acidity from streptococcal cultures. <it>P. gingivalis</it> containing samples had a reduction in levels of proteins for ethanol and formate but increased proteins for both acetate and lactate production. The communities also showed increases in exopolysaccharide synthesis, amino acid biosynthesis, and oxidative stress protection and decreases in adhesion and transporter proteins.</p> <p>Conclusion</p> <p>This study showed that <it>S. gordonii</it> demonstrates species specific responses during interactions with <it>F. nucleatum</it> or <it>P. gingivalis</it>. Extensive changes were seen in energy metabolism and byproduct production implicating nutrient transfer as an important community interaction.</p>http://www.biomedcentral.com/1471-2180/12/211<it>Streptococcus gordonii</it>Oral biofilmProteomicsModel community<it>Porphyromonas gingivalis</it><it>Fusobacterium nucleatum</it> |
| spellingShingle | Hendrickson Erik L Wang Tiansong Dickinson Brittany C Whitmore Sarah E Wright Christopher J Lamont Richard J Hackett Murray Proteomics of <it>Streptococcus gordonii</it> within a model developing oral microbial community BMC Microbiology <it>Streptococcus gordonii</it> Oral biofilm Proteomics Model community <it>Porphyromonas gingivalis</it> <it>Fusobacterium nucleatum</it> |
| title | Proteomics of <it>Streptococcus gordonii</it> within a model developing oral microbial community |
| title_full | Proteomics of <it>Streptococcus gordonii</it> within a model developing oral microbial community |
| title_fullStr | Proteomics of <it>Streptococcus gordonii</it> within a model developing oral microbial community |
| title_full_unstemmed | Proteomics of <it>Streptococcus gordonii</it> within a model developing oral microbial community |
| title_short | Proteomics of <it>Streptococcus gordonii</it> within a model developing oral microbial community |
| title_sort | proteomics of it streptococcus gordonii it within a model developing oral microbial community |
| topic | <it>Streptococcus gordonii</it> Oral biofilm Proteomics Model community <it>Porphyromonas gingivalis</it> <it>Fusobacterium nucleatum</it> |
| url | http://www.biomedcentral.com/1471-2180/12/211 |
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