Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis
The larvae of Echinococcus multilocularis causes alveolar echinococcosis, which poses a great threat to the public health. However, the molecular mechanisms underlying the host and parasite interactions are still unclear. Exploring the transcriptomic maps of mRNA, miRNA and lncRNA expressed in the l...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-03-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.798551/full |
| _version_ | 1818312262429966336 |
|---|---|
| author | Tingli Liu Hong Li Yanping Li Liqun Wang Guoliang Chen Guiting Pu Xiaola Guo William C. Cho Majid Fasihi Harandi Yadong Zheng Xuenong Luo |
| author_facet | Tingli Liu Hong Li Yanping Li Liqun Wang Guoliang Chen Guiting Pu Xiaola Guo William C. Cho Majid Fasihi Harandi Yadong Zheng Xuenong Luo |
| author_sort | Tingli Liu |
| collection | DOAJ |
| description | The larvae of Echinococcus multilocularis causes alveolar echinococcosis, which poses a great threat to the public health. However, the molecular mechanisms underlying the host and parasite interactions are still unclear. Exploring the transcriptomic maps of mRNA, miRNA and lncRNA expressed in the liver in response to E. multilocularis infection will help us to understand its pathogenesis. Using liver perfusion, different cell populations including the hepatic cells, hepatic stellate cells and Kupffer cells were isolated from mice interperitoneally inoculated with protoscoleces. Their transcriptional profiles including lncRNAs, miRNAs and mRNAs were done by RNA-seq. Among these cell populations, the most differentially-expressed (DE) mRNA, lncRNAs and miRNAs were annotated and may involve in the pathological processes, mainly including metabolic disorders, immune responses and liver fibrosis. Following the integrative analysis of 38 differentially-expressed DEmiRNAs and 8 DElncRNAs, the lncRNA-mRNA-miRNA networks were constructed, including F63-miR-223-3p-Fbxw7/ZFP36/map1b, F63-miR-27-5p-Tdrd6/Dip2c/Wdfy4 and IFNgAS1-IFN-γ. These results unveil the presence of several potential lncRNA-mRNA-miRNA axes during E. multilocularis infection, and further exploring of these axes may contribute to better understanding of the pathogenic mechanisms. |
| first_indexed | 2024-12-13T08:15:03Z |
| format | Article |
| id | doaj.art-7ff83c18bd14483e8db3e19e0928e632 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-12-13T08:15:03Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-7ff83c18bd14483e8db3e19e0928e6322022-12-21T23:54:07ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-03-011010.3389/fcell.2022.798551798551Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularisTingli Liu0Hong Li1Yanping Li2Liqun Wang3Guoliang Chen4Guiting Pu5Xiaola Guo6William C. Cho7Majid Fasihi Harandi8Yadong Zheng9Xuenong Luo10State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, ChinaDepartment of Clinical Oncology, Queen Elizabeth Hospital, Hong Kong SAR, ChinaResearch Center for Hydatid Disease in Iran, Kerman University of Medical Sciences, Kerman, IranKey Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology and College of Veterinary Medicine of Zhejiang A&F University, Hangzhou, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, CAAS, Lanzhou, ChinaThe larvae of Echinococcus multilocularis causes alveolar echinococcosis, which poses a great threat to the public health. However, the molecular mechanisms underlying the host and parasite interactions are still unclear. Exploring the transcriptomic maps of mRNA, miRNA and lncRNA expressed in the liver in response to E. multilocularis infection will help us to understand its pathogenesis. Using liver perfusion, different cell populations including the hepatic cells, hepatic stellate cells and Kupffer cells were isolated from mice interperitoneally inoculated with protoscoleces. Their transcriptional profiles including lncRNAs, miRNAs and mRNAs were done by RNA-seq. Among these cell populations, the most differentially-expressed (DE) mRNA, lncRNAs and miRNAs were annotated and may involve in the pathological processes, mainly including metabolic disorders, immune responses and liver fibrosis. Following the integrative analysis of 38 differentially-expressed DEmiRNAs and 8 DElncRNAs, the lncRNA-mRNA-miRNA networks were constructed, including F63-miR-223-3p-Fbxw7/ZFP36/map1b, F63-miR-27-5p-Tdrd6/Dip2c/Wdfy4 and IFNgAS1-IFN-γ. These results unveil the presence of several potential lncRNA-mRNA-miRNA axes during E. multilocularis infection, and further exploring of these axes may contribute to better understanding of the pathogenic mechanisms.https://www.frontiersin.org/articles/10.3389/fcell.2022.798551/fullEchinococcus multilocularishepatic cellshepatic stellate cellskupffer cellsregulatory network |
| spellingShingle | Tingli Liu Hong Li Yanping Li Liqun Wang Guoliang Chen Guiting Pu Xiaola Guo William C. Cho Majid Fasihi Harandi Yadong Zheng Xuenong Luo Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis Frontiers in Cell and Developmental Biology Echinococcus multilocularis hepatic cells hepatic stellate cells kupffer cells regulatory network |
| title | Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis |
| title_full | Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis |
| title_fullStr | Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis |
| title_full_unstemmed | Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis |
| title_short | Integrative Analysis of RNA Expression and Regulatory Networks in Mice Liver Infected by Echinococcus multilocularis |
| title_sort | integrative analysis of rna expression and regulatory networks in mice liver infected by echinococcus multilocularis |
| topic | Echinococcus multilocularis hepatic cells hepatic stellate cells kupffer cells regulatory network |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.798551/full |
| work_keys_str_mv | AT tingliliu integrativeanalysisofrnaexpressionandregulatorynetworksinmiceliverinfectedbyechinococcusmultilocularis AT hongli integrativeanalysisofrnaexpressionandregulatorynetworksinmiceliverinfectedbyechinococcusmultilocularis AT yanpingli integrativeanalysisofrnaexpressionandregulatorynetworksinmiceliverinfectedbyechinococcusmultilocularis AT liqunwang integrativeanalysisofrnaexpressionandregulatorynetworksinmiceliverinfectedbyechinococcusmultilocularis AT guoliangchen integrativeanalysisofrnaexpressionandregulatorynetworksinmiceliverinfectedbyechinococcusmultilocularis AT guitingpu integrativeanalysisofrnaexpressionandregulatorynetworksinmiceliverinfectedbyechinococcusmultilocularis AT xiaolaguo integrativeanalysisofrnaexpressionandregulatorynetworksinmiceliverinfectedbyechinococcusmultilocularis AT williamccho integrativeanalysisofrnaexpressionandregulatorynetworksinmiceliverinfectedbyechinococcusmultilocularis AT majidfasihiharandi integrativeanalysisofrnaexpressionandregulatorynetworksinmiceliverinfectedbyechinococcusmultilocularis AT yadongzheng integrativeanalysisofrnaexpressionandregulatorynetworksinmiceliverinfectedbyechinococcusmultilocularis AT xuenongluo integrativeanalysisofrnaexpressionandregulatorynetworksinmiceliverinfectedbyechinococcusmultilocularis |