Quantitative Bio-Imaging Tools to Dissect the Interplay of Membrane and Cytoskeletal Actin Dynamics in Immune Cells
Cellular function is reliant on the dynamic interplay between the plasma membrane and the actin cytoskeleton. This critical relationship is of particular importance in immune cells, where both the cytoskeleton and the plasma membrane work in concert to organize and potentiate immune signaling events...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-01-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2020.612542/full |
| _version_ | 1818389590190325760 |
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| author | Falk Schneider Huw Colin-York Huw Colin-York Marco Fritzsche Marco Fritzsche Marco Fritzsche |
| author_facet | Falk Schneider Huw Colin-York Huw Colin-York Marco Fritzsche Marco Fritzsche Marco Fritzsche |
| author_sort | Falk Schneider |
| collection | DOAJ |
| description | Cellular function is reliant on the dynamic interplay between the plasma membrane and the actin cytoskeleton. This critical relationship is of particular importance in immune cells, where both the cytoskeleton and the plasma membrane work in concert to organize and potentiate immune signaling events. Despite their importance, there remains a critical gap in understanding how these respective dynamics are coupled, and how this coupling in turn may influence immune cell function from the bottom up. In this review, we highlight recent optical technologies that could provide strategies to investigate the simultaneous dynamics of both the cytoskeleton and membrane as well as their interplay, focusing on current and future applications in immune cells. We provide a guide of the spatio-temporal scale of each technique as well as highlighting novel probes and labels that have the potential to provide insights into membrane and cytoskeletal dynamics. The quantitative biophysical tools presented here provide a new and exciting route to uncover the relationship between plasma membrane and cytoskeletal dynamics that underlies immune cell function. |
| first_indexed | 2024-12-14T04:44:09Z |
| format | Article |
| id | doaj.art-80033135faf44e1f90d153ce26386044 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-14T04:44:09Z |
| publishDate | 2021-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-80033135faf44e1f90d153ce263860442022-12-21T23:16:44ZengFrontiers Media S.A.Frontiers in Immunology1664-32242021-01-011110.3389/fimmu.2020.612542612542Quantitative Bio-Imaging Tools to Dissect the Interplay of Membrane and Cytoskeletal Actin Dynamics in Immune CellsFalk Schneider0Huw Colin-York1Huw Colin-York2Marco Fritzsche3Marco Fritzsche4Marco Fritzsche5Medical Research Council (MRC) Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United KingdomMedical Research Council (MRC) Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United KingdomKennedy Institute for Rheumatology, University of Oxford, Oxford, United KingdomMedical Research Council (MRC) Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United KingdomKennedy Institute for Rheumatology, University of Oxford, Oxford, United KingdomRosalind Franklin Institute, Harwell Campus, Didcot, United KingdomCellular function is reliant on the dynamic interplay between the plasma membrane and the actin cytoskeleton. This critical relationship is of particular importance in immune cells, where both the cytoskeleton and the plasma membrane work in concert to organize and potentiate immune signaling events. Despite their importance, there remains a critical gap in understanding how these respective dynamics are coupled, and how this coupling in turn may influence immune cell function from the bottom up. In this review, we highlight recent optical technologies that could provide strategies to investigate the simultaneous dynamics of both the cytoskeleton and membrane as well as their interplay, focusing on current and future applications in immune cells. We provide a guide of the spatio-temporal scale of each technique as well as highlighting novel probes and labels that have the potential to provide insights into membrane and cytoskeletal dynamics. The quantitative biophysical tools presented here provide a new and exciting route to uncover the relationship between plasma membrane and cytoskeletal dynamics that underlies immune cell function.https://www.frontiersin.org/articles/10.3389/fimmu.2020.612542/fullplasma membraneactin cytoskeletonfluorescence correlation spectroscopyfluorescence recovery after photobleachingimmune cellsmetal induced energy transfer |
| spellingShingle | Falk Schneider Huw Colin-York Huw Colin-York Marco Fritzsche Marco Fritzsche Marco Fritzsche Quantitative Bio-Imaging Tools to Dissect the Interplay of Membrane and Cytoskeletal Actin Dynamics in Immune Cells Frontiers in Immunology plasma membrane actin cytoskeleton fluorescence correlation spectroscopy fluorescence recovery after photobleaching immune cells metal induced energy transfer |
| title | Quantitative Bio-Imaging Tools to Dissect the Interplay of Membrane and Cytoskeletal Actin Dynamics in Immune Cells |
| title_full | Quantitative Bio-Imaging Tools to Dissect the Interplay of Membrane and Cytoskeletal Actin Dynamics in Immune Cells |
| title_fullStr | Quantitative Bio-Imaging Tools to Dissect the Interplay of Membrane and Cytoskeletal Actin Dynamics in Immune Cells |
| title_full_unstemmed | Quantitative Bio-Imaging Tools to Dissect the Interplay of Membrane and Cytoskeletal Actin Dynamics in Immune Cells |
| title_short | Quantitative Bio-Imaging Tools to Dissect the Interplay of Membrane and Cytoskeletal Actin Dynamics in Immune Cells |
| title_sort | quantitative bio imaging tools to dissect the interplay of membrane and cytoskeletal actin dynamics in immune cells |
| topic | plasma membrane actin cytoskeleton fluorescence correlation spectroscopy fluorescence recovery after photobleaching immune cells metal induced energy transfer |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2020.612542/full |
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