The unique hmuY gene sequence as a specific marker of Porphyromonas gingivalis.
Porphyromonas gingivalis, a major etiological agent of chronic periodontitis, acquires heme from host hemoproteins using the HmuY hemophore. The aim of this study was to develop a specific P. gingivalis marker based on a hmuY gene sequence. Subgingival samples were collected from 66 patients with ch...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3699645?pdf=render |
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| author | Anna Gmiterek Halina Wójtowicz Paweł Mackiewicz Małgorzata Radwan-Oczko Małgorzata Kantorowicz Maria Chomyszyn-Gajewska Magdalena Frąszczak Marcin Bielecki Mariusz Olczak Teresa Olczak |
| author_facet | Anna Gmiterek Halina Wójtowicz Paweł Mackiewicz Małgorzata Radwan-Oczko Małgorzata Kantorowicz Maria Chomyszyn-Gajewska Magdalena Frąszczak Marcin Bielecki Mariusz Olczak Teresa Olczak |
| author_sort | Anna Gmiterek |
| collection | DOAJ |
| description | Porphyromonas gingivalis, a major etiological agent of chronic periodontitis, acquires heme from host hemoproteins using the HmuY hemophore. The aim of this study was to develop a specific P. gingivalis marker based on a hmuY gene sequence. Subgingival samples were collected from 66 patients with chronic periodontitis and 40 healthy subjects and the entire hmuY gene was analyzed in positive samples. Phylogenetic analyses demonstrated that both the amino acid sequence of the HmuY protein and the nucleotide sequence of the hmuY gene are unique among P. gingivalis strains/isolates and show low identity to sequences found in other species (below 50 and 56%, respectively). In agreement with these findings, a set of hmuY gene-based primers and standard/real-time PCR with SYBR Green chemistry allowed us to specifically detect P. gingivalis in patients with chronic periodontitis (77.3%) and healthy subjects (20%), the latter possessing lower number of P. gingivalis cells and total bacterial cells. Isolates from healthy subjects possess the hmuY gene-based nucleotide sequence pattern occurring in W83/W50/A7436 (n = 4), 381/ATCC 33277 (n = 3) or TDC60 (n = 1) strains, whereas those from patients typically have TDC60 (n = 21), W83/W50/A7436 (n = 17) and 381/ATCC 33277 (n = 13) strains. We observed a significant correlation between periodontal index of risk of infectiousness (PIRI) and the presence/absence of P. gingivalis (regardless of the hmuY gene-based sequence pattern of the isolate identified [r = 0.43; P = 0.0002] and considering particular isolate pattern [r = 0.38; P = 0.0012]). In conclusion, we demonstrated that the hmuY gene sequence or its fragments may be used as one of the molecular markers of P. gingivalis. |
| first_indexed | 2024-12-12T11:54:36Z |
| format | Article |
| id | doaj.art-80035bd7e1284dc49f9708bd25502257 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T11:54:36Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-80035bd7e1284dc49f9708bd255022572022-12-22T00:25:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0187e6771910.1371/journal.pone.0067719The unique hmuY gene sequence as a specific marker of Porphyromonas gingivalis.Anna GmiterekHalina WójtowiczPaweł MackiewiczMałgorzata Radwan-OczkoMałgorzata KantorowiczMaria Chomyszyn-GajewskaMagdalena FrąszczakMarcin BieleckiMariusz OlczakTeresa OlczakPorphyromonas gingivalis, a major etiological agent of chronic periodontitis, acquires heme from host hemoproteins using the HmuY hemophore. The aim of this study was to develop a specific P. gingivalis marker based on a hmuY gene sequence. Subgingival samples were collected from 66 patients with chronic periodontitis and 40 healthy subjects and the entire hmuY gene was analyzed in positive samples. Phylogenetic analyses demonstrated that both the amino acid sequence of the HmuY protein and the nucleotide sequence of the hmuY gene are unique among P. gingivalis strains/isolates and show low identity to sequences found in other species (below 50 and 56%, respectively). In agreement with these findings, a set of hmuY gene-based primers and standard/real-time PCR with SYBR Green chemistry allowed us to specifically detect P. gingivalis in patients with chronic periodontitis (77.3%) and healthy subjects (20%), the latter possessing lower number of P. gingivalis cells and total bacterial cells. Isolates from healthy subjects possess the hmuY gene-based nucleotide sequence pattern occurring in W83/W50/A7436 (n = 4), 381/ATCC 33277 (n = 3) or TDC60 (n = 1) strains, whereas those from patients typically have TDC60 (n = 21), W83/W50/A7436 (n = 17) and 381/ATCC 33277 (n = 13) strains. We observed a significant correlation between periodontal index of risk of infectiousness (PIRI) and the presence/absence of P. gingivalis (regardless of the hmuY gene-based sequence pattern of the isolate identified [r = 0.43; P = 0.0002] and considering particular isolate pattern [r = 0.38; P = 0.0012]). In conclusion, we demonstrated that the hmuY gene sequence or its fragments may be used as one of the molecular markers of P. gingivalis.http://europepmc.org/articles/PMC3699645?pdf=render |
| spellingShingle | Anna Gmiterek Halina Wójtowicz Paweł Mackiewicz Małgorzata Radwan-Oczko Małgorzata Kantorowicz Maria Chomyszyn-Gajewska Magdalena Frąszczak Marcin Bielecki Mariusz Olczak Teresa Olczak The unique hmuY gene sequence as a specific marker of Porphyromonas gingivalis. PLoS ONE |
| title | The unique hmuY gene sequence as a specific marker of Porphyromonas gingivalis. |
| title_full | The unique hmuY gene sequence as a specific marker of Porphyromonas gingivalis. |
| title_fullStr | The unique hmuY gene sequence as a specific marker of Porphyromonas gingivalis. |
| title_full_unstemmed | The unique hmuY gene sequence as a specific marker of Porphyromonas gingivalis. |
| title_short | The unique hmuY gene sequence as a specific marker of Porphyromonas gingivalis. |
| title_sort | unique hmuy gene sequence as a specific marker of porphyromonas gingivalis |
| url | http://europepmc.org/articles/PMC3699645?pdf=render |
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