Silk sericin alleviates aberrant photoperiod-induced alterations in testicular and adrenal steroidogenesis in adult mice
Abstract Background Steroidogenesis is a complex process of sequential enzymatic reactions affected by climate change. Animals respond to altered day length, the so-called photoperiod, with changes in physiology. The study aimed to an evaluation of sericin effect in alleviating steroidogenesis disor...
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | Reproductive Biology and Endocrinology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12958-022-01032-y |
| _version_ | 1797983901815144448 |
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| author | Eman Hassan Shahinaz Magdy Amany Attaallah Eman Gaber Omnia Mansour Rehab A. Gomaa Hala Odessy Maria Augustyniak Lamia M. El-Samad Abeer El Wakil |
| author_facet | Eman Hassan Shahinaz Magdy Amany Attaallah Eman Gaber Omnia Mansour Rehab A. Gomaa Hala Odessy Maria Augustyniak Lamia M. El-Samad Abeer El Wakil |
| author_sort | Eman Hassan |
| collection | DOAJ |
| description | Abstract Background Steroidogenesis is a complex process of sequential enzymatic reactions affected by climate change. Animals respond to altered day length, the so-called photoperiod, with changes in physiology. The study aimed to an evaluation of sericin effect in alleviating steroidogenesis disorders induced by disturbed photoperiod in mice. Methods The animals were randomly divided into three groups according to the lighting cycle: a control group with a standard 12Light:12Dark cycle, a short-term photoperiod group with a 6Light:18Dark cycle, and a long-term photoperiod group with an 18Light:6Dark cycle. Both short and long-term groups were subdivided into two equal subgroups: The placebo and the sericin-treated subgroups received, for five weeks from prepubertal throughout adulthood, one intraperitoneal injection per week of the solvent and 1 g sericin/kg body weight, respectively. Results Selected oxidative stress parameters and testicular and adrenal steroidogenic capacities of adult mice were measured. After five weeks, the placebo group with impaired photoperiod showed a decrease in the quality and quantity of sperm and a reduction in testosterone, corticosterone, aldosterone, total antioxidant capacity, xanthine oxidase, and melatonin. At the same time, in these groups, there was an increase in the level of aromatase, malondialdehyde, cholesterol, and steroidogenic factor-1 (SF-1) expression in the adrenal cortex and an enhancement in histological lesions. Mice receiving sericin had parameters similar to the control group. Conclusion Our findings reveal that silk sericin can reduce the stress caused by photoperiod disorders regarding testicular function, sex hormone levels, and sperm quantity and quality. Thus, sericin is a biocompatible protein with a promising potential for its use in the case of organisms living under an abnormal photoperiod. |
| first_indexed | 2024-04-11T06:53:30Z |
| format | Article |
| id | doaj.art-80042423a1b046ffade55866494327ff |
| institution | Directory Open Access Journal |
| issn | 1477-7827 |
| language | English |
| last_indexed | 2024-04-11T06:53:30Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Reproductive Biology and Endocrinology |
| spelling | doaj.art-80042423a1b046ffade55866494327ff2022-12-22T04:39:05ZengBMCReproductive Biology and Endocrinology1477-78272022-11-0120111510.1186/s12958-022-01032-ySilk sericin alleviates aberrant photoperiod-induced alterations in testicular and adrenal steroidogenesis in adult miceEman Hassan0Shahinaz Magdy1Amany Attaallah2Eman Gaber3Omnia Mansour4Rehab A. Gomaa5Hala Odessy6Maria Augustyniak7Lamia M. El-Samad8Abeer El Wakil9Department of Biological and Geological Sciences, Faculty of Education, Alexandria UniversityDepartment of Biological and Geological Sciences, Faculty of Education, Alexandria UniversityDepartment of Zoology, Faculty of Science, Damanhour UniversityDepartment of Biological and Geological Sciences, Faculty of Education, Alexandria UniversityDepartment of Biological and Geological Sciences, Faculty of Education, Alexandria UniversityDepartment of Zoology, Faculty of Science, Alexandria UniversityDepartment of Biological and Geological Sciences, Faculty of Education, Alexandria UniversityInstitute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in KatowiceDepartment of Zoology, Faculty of Science, Alexandria UniversityDepartment of Biological and Geological Sciences, Faculty of Education, Alexandria UniversityAbstract Background Steroidogenesis is a complex process of sequential enzymatic reactions affected by climate change. Animals respond to altered day length, the so-called photoperiod, with changes in physiology. The study aimed to an evaluation of sericin effect in alleviating steroidogenesis disorders induced by disturbed photoperiod in mice. Methods The animals were randomly divided into three groups according to the lighting cycle: a control group with a standard 12Light:12Dark cycle, a short-term photoperiod group with a 6Light:18Dark cycle, and a long-term photoperiod group with an 18Light:6Dark cycle. Both short and long-term groups were subdivided into two equal subgroups: The placebo and the sericin-treated subgroups received, for five weeks from prepubertal throughout adulthood, one intraperitoneal injection per week of the solvent and 1 g sericin/kg body weight, respectively. Results Selected oxidative stress parameters and testicular and adrenal steroidogenic capacities of adult mice were measured. After five weeks, the placebo group with impaired photoperiod showed a decrease in the quality and quantity of sperm and a reduction in testosterone, corticosterone, aldosterone, total antioxidant capacity, xanthine oxidase, and melatonin. At the same time, in these groups, there was an increase in the level of aromatase, malondialdehyde, cholesterol, and steroidogenic factor-1 (SF-1) expression in the adrenal cortex and an enhancement in histological lesions. Mice receiving sericin had parameters similar to the control group. Conclusion Our findings reveal that silk sericin can reduce the stress caused by photoperiod disorders regarding testicular function, sex hormone levels, and sperm quantity and quality. Thus, sericin is a biocompatible protein with a promising potential for its use in the case of organisms living under an abnormal photoperiod.https://doi.org/10.1186/s12958-022-01032-yPhotoperiodismEnvironmental stressorSteroidogenesisAdrenal glandTestis |
| spellingShingle | Eman Hassan Shahinaz Magdy Amany Attaallah Eman Gaber Omnia Mansour Rehab A. Gomaa Hala Odessy Maria Augustyniak Lamia M. El-Samad Abeer El Wakil Silk sericin alleviates aberrant photoperiod-induced alterations in testicular and adrenal steroidogenesis in adult mice Reproductive Biology and Endocrinology Photoperiodism Environmental stressor Steroidogenesis Adrenal gland Testis |
| title | Silk sericin alleviates aberrant photoperiod-induced alterations in testicular and adrenal steroidogenesis in adult mice |
| title_full | Silk sericin alleviates aberrant photoperiod-induced alterations in testicular and adrenal steroidogenesis in adult mice |
| title_fullStr | Silk sericin alleviates aberrant photoperiod-induced alterations in testicular and adrenal steroidogenesis in adult mice |
| title_full_unstemmed | Silk sericin alleviates aberrant photoperiod-induced alterations in testicular and adrenal steroidogenesis in adult mice |
| title_short | Silk sericin alleviates aberrant photoperiod-induced alterations in testicular and adrenal steroidogenesis in adult mice |
| title_sort | silk sericin alleviates aberrant photoperiod induced alterations in testicular and adrenal steroidogenesis in adult mice |
| topic | Photoperiodism Environmental stressor Steroidogenesis Adrenal gland Testis |
| url | https://doi.org/10.1186/s12958-022-01032-y |
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