Biological Activity and Antibiofilm Molecular Profile of <i>Citrus aurantium</i> Essential Oil and Its Application in a Food Model
The main aim of the study was to investigate the chemical composition, antioxidant, antimicrobial, and antibiofilm activity of <i>Citrus aurantium</i> essential oil (CAEO). The biofilm profile of <i>Stenotrophonomonas maltophilia</i> and <i>Bacillus subtilis</i> w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/17/3956 |
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| author | Miroslava Kačániová Margarita Terentjeva Lucia Galovičová Eva Ivanišová Jana Štefániková Veronika Valková Petra Borotová Przemysław Łukasz Kowalczewski Simona Kunová Soňa Felšöciová Eva Tvrdá Jana Žiarovská Renáta Benda Prokeinová Nenad Vukovic |
| author_facet | Miroslava Kačániová Margarita Terentjeva Lucia Galovičová Eva Ivanišová Jana Štefániková Veronika Valková Petra Borotová Przemysław Łukasz Kowalczewski Simona Kunová Soňa Felšöciová Eva Tvrdá Jana Žiarovská Renáta Benda Prokeinová Nenad Vukovic |
| author_sort | Miroslava Kačániová |
| collection | DOAJ |
| description | The main aim of the study was to investigate the chemical composition, antioxidant, antimicrobial, and antibiofilm activity of <i>Citrus aurantium</i> essential oil (CAEO). The biofilm profile of <i>Stenotrophonomonas maltophilia</i> and <i>Bacillus subtilis</i> were assessed using the mass spectrometry MALDI-TOF MS Biotyper and the antibiofilm activity of <i>Citrus aurantium</i> (CAEO) was studied on wood and glass surfaces. A semi-quantitative composition using a modified version was applied for the CAEO characterization. The antioxidant activity of CAEO was determined using the DPPH method. The antimicrobial activity was analyzed by disc diffusion for two biofilm producing bacteria, while the vapor phase was used for three penicillia. The antibiofilm activity was observed with the agar microdilution method. The molecular differences of biofilm formation on different days were analyzed, and the genetic similarity was studied with dendrograms constructed from MSP spectra to illustrate the grouping profiles of <i>S. maltophilia</i> and <i>B. subtilis</i>. A differentiated branch was obtained for early growth variants of <i>S. maltophilia</i> for planktonic cells and all experimental groups. The time span can be reported for the grouping pattern of <i>B. subtilis</i> preferentially when comparing to the media matrix, but without clear differences among variants. Furthermore, the minimum inhibitory doses of the CAEO were investigated against microscopic fungi. The results showed that CAEO was most active against <i>Penicillium crustosum</i>, in the vapor phase, on bread and carrot in situ. |
| first_indexed | 2024-03-10T16:42:58Z |
| format | Article |
| id | doaj.art-80075d525a6242438f2ffbcc1800dc91 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T16:42:58Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-80075d525a6242438f2ffbcc1800dc912023-11-20T11:53:47ZengMDPI AGMolecules1420-30492020-08-012517395610.3390/molecules25173956Biological Activity and Antibiofilm Molecular Profile of <i>Citrus aurantium</i> Essential Oil and Its Application in a Food ModelMiroslava Kačániová0Margarita Terentjeva1Lucia Galovičová2Eva Ivanišová3Jana Štefániková4Veronika Valková5Petra Borotová6Przemysław Łukasz Kowalczewski7Simona Kunová8Soňa Felšöciová9Eva Tvrdá10Jana Žiarovská11Renáta Benda Prokeinová12Nenad Vukovic13Department of Fruit Science, Viticulture and Enology, Faculty of Horticulture and Landscape Engineering, Slovak University of Agriculture, Tr. A. Hlinku 2, 94976 Nitra, SlovakiaInstitute of Food and Environmental Hygiene, Faculty of Veterinary Medicine, Latvia University of Life Sciences and Technologies, K. Helmaņaiela 8, LV-3004 Jelgava, LatviaDepartment of Fruit Science, Viticulture and Enology, Faculty of Horticulture and Landscape Engineering, Slovak University of Agriculture, Tr. A. Hlinku 2, 94976 Nitra, SlovakiaDepartment of Technology and Quality of Plant Products, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 94976 Nitra, SlovakiaAgroBioTech Research Centre, Slovak University of Agriculture, Tr. A. Hlinku 2, 94976 Nitra, SlovakiaDepartment of Fruit Science, Viticulture and Enology, Faculty of Horticulture and Landscape Engineering, Slovak University of Agriculture, Tr. A. Hlinku 2, 94976 Nitra, SlovakiaAgroBioTech Research Centre, Slovak University of Agriculture, Tr. A. Hlinku 2, 94976 Nitra, SlovakiaInstitute of Food Technology of Plant Origin, Poznań University of Life Sciences, 31 Wojska Polskiego St., 60-624 Poznań, PolandDepartment of Food Hygiene and Safety, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaDepartment of Microbiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaDepartment of Animal Physiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaDepartment of Plant Genetics and Breeding, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaDepartment of Statistics and Operations Research, Faculty of Economic and Management, Slovak University of Agriculture, Tr. A. Hlinku 2, 949 76 Nitra, SlovakiaDepartment of Chemistry, Faculty of Science, University of Kragujevac, P.O. Box 12, 34000 Kragujevac, SerbiaThe main aim of the study was to investigate the chemical composition, antioxidant, antimicrobial, and antibiofilm activity of <i>Citrus aurantium</i> essential oil (CAEO). The biofilm profile of <i>Stenotrophonomonas maltophilia</i> and <i>Bacillus subtilis</i> were assessed using the mass spectrometry MALDI-TOF MS Biotyper and the antibiofilm activity of <i>Citrus aurantium</i> (CAEO) was studied on wood and glass surfaces. A semi-quantitative composition using a modified version was applied for the CAEO characterization. The antioxidant activity of CAEO was determined using the DPPH method. The antimicrobial activity was analyzed by disc diffusion for two biofilm producing bacteria, while the vapor phase was used for three penicillia. The antibiofilm activity was observed with the agar microdilution method. The molecular differences of biofilm formation on different days were analyzed, and the genetic similarity was studied with dendrograms constructed from MSP spectra to illustrate the grouping profiles of <i>S. maltophilia</i> and <i>B. subtilis</i>. A differentiated branch was obtained for early growth variants of <i>S. maltophilia</i> for planktonic cells and all experimental groups. The time span can be reported for the grouping pattern of <i>B. subtilis</i> preferentially when comparing to the media matrix, but without clear differences among variants. Furthermore, the minimum inhibitory doses of the CAEO were investigated against microscopic fungi. The results showed that CAEO was most active against <i>Penicillium crustosum</i>, in the vapor phase, on bread and carrot in situ.https://www.mdpi.com/1420-3049/25/17/3956bitter orange essential oilbiological activity<i>Stenotrophomonas maltophilia</i><i>Bacillus subtilis</i><i>Penicillinum</i>glass |
| spellingShingle | Miroslava Kačániová Margarita Terentjeva Lucia Galovičová Eva Ivanišová Jana Štefániková Veronika Valková Petra Borotová Przemysław Łukasz Kowalczewski Simona Kunová Soňa Felšöciová Eva Tvrdá Jana Žiarovská Renáta Benda Prokeinová Nenad Vukovic Biological Activity and Antibiofilm Molecular Profile of <i>Citrus aurantium</i> Essential Oil and Its Application in a Food Model Molecules bitter orange essential oil biological activity <i>Stenotrophomonas maltophilia</i> <i>Bacillus subtilis</i> <i>Penicillinum</i> glass |
| title | Biological Activity and Antibiofilm Molecular Profile of <i>Citrus aurantium</i> Essential Oil and Its Application in a Food Model |
| title_full | Biological Activity and Antibiofilm Molecular Profile of <i>Citrus aurantium</i> Essential Oil and Its Application in a Food Model |
| title_fullStr | Biological Activity and Antibiofilm Molecular Profile of <i>Citrus aurantium</i> Essential Oil and Its Application in a Food Model |
| title_full_unstemmed | Biological Activity and Antibiofilm Molecular Profile of <i>Citrus aurantium</i> Essential Oil and Its Application in a Food Model |
| title_short | Biological Activity and Antibiofilm Molecular Profile of <i>Citrus aurantium</i> Essential Oil and Its Application in a Food Model |
| title_sort | biological activity and antibiofilm molecular profile of i citrus aurantium i essential oil and its application in a food model |
| topic | bitter orange essential oil biological activity <i>Stenotrophomonas maltophilia</i> <i>Bacillus subtilis</i> <i>Penicillinum</i> glass |
| url | https://www.mdpi.com/1420-3049/25/17/3956 |
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