High-risk human papillomaviruses L1 gene isolates identified in Western Kazakhstan
Kazakhstani researchers reported a significant prevalence of highly carcinogenic human papillomavirus types in the country. The article aimed to present HPV L1 gene sequencing developments in women affected with cervical cancer throughout the western part of Kazakhstan with provided findings on the...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
National Scientific Medical Center
2024-02-01
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| Series: | Ķazaķstannyṇ Klinikalyķ Medicinasy |
| Subjects: | |
| Online Access: | https://www.clinmedkaz.org/download/high-risk-human-papillomaviruses-l1-gene-isolates-identified-in-western-kazakhstan-14274.pdf |
| _version_ | 1797245057482358784 |
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| author | Saule K. Balmagambetova Elena V. Zholdybayeva Oxana V. Zavalennaya Ainur Amanzholkyzy Victoria I. Kononets Gulmira M. Zharmakhanova Nadiar M. Mussin Lazzat M. Zhamaliyeva Nurgul M. Kereyeva |
| author_facet | Saule K. Balmagambetova Elena V. Zholdybayeva Oxana V. Zavalennaya Ainur Amanzholkyzy Victoria I. Kononets Gulmira M. Zharmakhanova Nadiar M. Mussin Lazzat M. Zhamaliyeva Nurgul M. Kereyeva |
| author_sort | Saule K. Balmagambetova |
| collection | DOAJ |
| description | Kazakhstani researchers reported a significant prevalence of highly carcinogenic human papillomavirus types in the country.
The article aimed to present HPV L1 gene sequencing developments in women affected with cervical cancer throughout the western part of Kazakhstan with provided findings on the geographic pathways of obtained isolates.
Methods. The HPV L1 gene was amplified using the consensus primers MY09HPV 5’-CGTCCMARRGGAWACTGATC-3’ and MY11HPV 5’ – GCMCAGGGWCATAAYAATGG-3’. The purified DNA was used as the target for direct nucleotide sequencing. Phylogenetic analyses were conducted using the MegAlign program from the LASERGENE software package (version 6.0; DNA star, Madison, WI) and with MEGA version 5.0 software. A multiple alignment was created through Clustal W software, and the neighbor-joining method was used to construct the phylogenetic tree.
Results. Of 70 HPV samples transported to the Astana shared laboratory for gene L1 sequencing, only ten appeared fit to obtain isolates (14.3%). The viral load of the samples ranged from 3.3 to 8.2, and the range of DNA concentration was from 8.16 to 69.6 ng/uL. HPV16 unique Kazakhstani isolate from Aktobe, having its own branch, and not yet registered in the world genebank, was revealed. An isolate of potentially carcinogenic HPV53 forming a remote cluster with KF436822/1, KU951264.1 - Southwest China, and 97% identity with EU056643.1 - Ireland, and acted as a single agent for invasive cervical cancer was identified.
In general, the sequencing findings indicate the variety of ways for HPV pervasion into the western region of Kazakhstan: North and South America, Europe, and Asia.
The study was recorded in the ISRCTN registry, No. 7154910, 02/01/2018. |
| first_indexed | 2024-04-24T19:20:51Z |
| format | Article |
| id | doaj.art-8008ca19625847d79400fbb1b50d90d0 |
| institution | Directory Open Access Journal |
| issn | 1812-2892 2313-1519 |
| language | English |
| last_indexed | 2024-04-24T19:20:51Z |
| publishDate | 2024-02-01 |
| publisher | National Scientific Medical Center |
| record_format | Article |
| series | Ķazaķstannyṇ Klinikalyķ Medicinasy |
| spelling | doaj.art-8008ca19625847d79400fbb1b50d90d02024-03-25T18:48:56ZengNational Scientific Medical CenterĶazaķstannyṇ Klinikalyķ Medicinasy1812-28922313-15192024-02-01211263410.23950/jcmk/14274High-risk human papillomaviruses L1 gene isolates identified in Western KazakhstanSaule K. Balmagambetova0Elena V. Zholdybayeva1Oxana V. Zavalennaya2Ainur Amanzholkyzy3Victoria I. Kononets4Gulmira M. Zharmakhanova5Nadiar M. Mussin6Lazzat M. Zhamaliyeva7Nurgul M. Kereyeva8Department of Oncology, West Kazakhstan Marat Ospanov Medical University, Aktobe, KazakhstanShared laboratory, National Center for Biotechnology, Astana, KazakhstanPCR laboratory, Scientific-Production Center, West Kazakhstan Marat Ospanov Medical University, Aktobe, KazakhstanDepartment of Physiology, West Kazakhstan Marat Ospanov Medical University, Aktobe, KazakhstanDepartment of Natural Science Disciplines, West Kazakhstan Marat Ospanov Medical University, Aktobe, KazakhstanDepartment of Natural Science Disciplines, West Kazakhstan Marat Ospanov Medical University, Aktobe, KazakhstanDepartment of Transplantology, West Kazakhstan Marat Ospanov Medical University, Aktobe, KazakhstanDepartment of Family Medicine, West Kazakhstan Marat Ospanov Medical University, Aktobe, KazakhstanDepartment of Oncology, West Kazakhstan Marat Ospanov Medical University, Aktobe, KazakhstanKazakhstani researchers reported a significant prevalence of highly carcinogenic human papillomavirus types in the country. The article aimed to present HPV L1 gene sequencing developments in women affected with cervical cancer throughout the western part of Kazakhstan with provided findings on the geographic pathways of obtained isolates. Methods. The HPV L1 gene was amplified using the consensus primers MY09HPV 5’-CGTCCMARRGGAWACTGATC-3’ and MY11HPV 5’ – GCMCAGGGWCATAAYAATGG-3’. The purified DNA was used as the target for direct nucleotide sequencing. Phylogenetic analyses were conducted using the MegAlign program from the LASERGENE software package (version 6.0; DNA star, Madison, WI) and with MEGA version 5.0 software. A multiple alignment was created through Clustal W software, and the neighbor-joining method was used to construct the phylogenetic tree. Results. Of 70 HPV samples transported to the Astana shared laboratory for gene L1 sequencing, only ten appeared fit to obtain isolates (14.3%). The viral load of the samples ranged from 3.3 to 8.2, and the range of DNA concentration was from 8.16 to 69.6 ng/uL. HPV16 unique Kazakhstani isolate from Aktobe, having its own branch, and not yet registered in the world genebank, was revealed. An isolate of potentially carcinogenic HPV53 forming a remote cluster with KF436822/1, KU951264.1 - Southwest China, and 97% identity with EU056643.1 - Ireland, and acted as a single agent for invasive cervical cancer was identified. In general, the sequencing findings indicate the variety of ways for HPV pervasion into the western region of Kazakhstan: North and South America, Europe, and Asia. The study was recorded in the ISRCTN registry, No. 7154910, 02/01/2018.https://www.clinmedkaz.org/download/high-risk-human-papillomaviruses-l1-gene-isolates-identified-in-western-kazakhstan-14274.pdfHuman papillomavirusgene sequencingisolatesgene L1cervical cancerwestern Kazakhstan. |
| spellingShingle | Saule K. Balmagambetova Elena V. Zholdybayeva Oxana V. Zavalennaya Ainur Amanzholkyzy Victoria I. Kononets Gulmira M. Zharmakhanova Nadiar M. Mussin Lazzat M. Zhamaliyeva Nurgul M. Kereyeva High-risk human papillomaviruses L1 gene isolates identified in Western Kazakhstan Ķazaķstannyṇ Klinikalyķ Medicinasy Human papillomavirus gene sequencing isolates gene L1 cervical cancer western Kazakhstan. |
| title | High-risk human papillomaviruses L1 gene isolates identified in Western Kazakhstan |
| title_full | High-risk human papillomaviruses L1 gene isolates identified in Western Kazakhstan |
| title_fullStr | High-risk human papillomaviruses L1 gene isolates identified in Western Kazakhstan |
| title_full_unstemmed | High-risk human papillomaviruses L1 gene isolates identified in Western Kazakhstan |
| title_short | High-risk human papillomaviruses L1 gene isolates identified in Western Kazakhstan |
| title_sort | high risk human papillomaviruses l1 gene isolates identified in western kazakhstan |
| topic | Human papillomavirus gene sequencing isolates gene L1 cervical cancer western Kazakhstan. |
| url | https://www.clinmedkaz.org/download/high-risk-human-papillomaviruses-l1-gene-isolates-identified-in-western-kazakhstan-14274.pdf |
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