Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A
Jujubosides are the major medicinal ingredients of Ziziphi Spinosae Semen (the seed of wild jujube). To date, a complete understanding of jujuboside’s metabolic pathways has not been attained. This study has systematically identified 35 β-glucosidase genes belonging to the glycoside hydrolase family...
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MDPI AG
2023-05-01
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| author | Mingjun Yang Yimian Ma Xupeng Si Xiaofeng Liu Xin Geng Xin Wen Guoqiong Li Liping Zhang Chengmin Yang Zheng Zhang |
| author_facet | Mingjun Yang Yimian Ma Xupeng Si Xiaofeng Liu Xin Geng Xin Wen Guoqiong Li Liping Zhang Chengmin Yang Zheng Zhang |
| author_sort | Mingjun Yang |
| collection | DOAJ |
| description | Jujubosides are the major medicinal ingredients of Ziziphi Spinosae Semen (the seed of wild jujube). To date, a complete understanding of jujuboside’s metabolic pathways has not been attained. This study has systematically identified 35 β-glucosidase genes belonging to the glycoside hydrolase family 1 (GH1) using bioinformatic methods based on the wild jujube genome. The conserved domains and motifs of the 35 putative β-glucosidases, along with the genome locations and exon–intron structures of 35 β-glucosidase genes were revealed. The potential functions of the putative proteins encoded by the 35 β-glucosidase genes are suggested based on their phylogenetic relationships with <i>Arabidopsis</i> homologs. Two wild jujube β-glucosidase genes were heterologously expressed in <i>Escherichia coli</i>, and the recombinant proteins were able to convert jujuboside A (JuA) into jujuboside B (JuB). Since it has been previously reported that JuA catabolites, including JuB and other rare jujubosides, may play crucial roles in the jujuboside’s pharmacological activity, it is suggested that these two proteins can be used to enhance the utilization potential of jujubosides. This study provides new insight into the metabolism of jujubosides in wild jujube. Furthermore, the characterization of β-glucosidase genes is expected to facilitate investigations involving the cultivation and breeding of wild jujube. |
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| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T02:26:27Z |
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| series | Genes |
| spelling | doaj.art-800e3c6b3b2e48c6823bd7ea5bb365832023-11-18T10:33:18ZengMDPI AGGenes2073-44252023-05-01146113510.3390/genes14061135Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside AMingjun Yang0Yimian Ma1Xupeng Si2Xiaofeng Liu3Xin Geng4Xin Wen5Guoqiong Li6Liping Zhang7Chengmin Yang8Zheng Zhang9School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, ChinaNational Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaSchool of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, ChinaSchool of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, ChinaNational Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaNational Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaNational Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaNational Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaNational Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaNational Engineering Laboratory for Breeding of Endangered Medicinal Materials, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, ChinaJujubosides are the major medicinal ingredients of Ziziphi Spinosae Semen (the seed of wild jujube). To date, a complete understanding of jujuboside’s metabolic pathways has not been attained. This study has systematically identified 35 β-glucosidase genes belonging to the glycoside hydrolase family 1 (GH1) using bioinformatic methods based on the wild jujube genome. The conserved domains and motifs of the 35 putative β-glucosidases, along with the genome locations and exon–intron structures of 35 β-glucosidase genes were revealed. The potential functions of the putative proteins encoded by the 35 β-glucosidase genes are suggested based on their phylogenetic relationships with <i>Arabidopsis</i> homologs. Two wild jujube β-glucosidase genes were heterologously expressed in <i>Escherichia coli</i>, and the recombinant proteins were able to convert jujuboside A (JuA) into jujuboside B (JuB). Since it has been previously reported that JuA catabolites, including JuB and other rare jujubosides, may play crucial roles in the jujuboside’s pharmacological activity, it is suggested that these two proteins can be used to enhance the utilization potential of jujubosides. This study provides new insight into the metabolism of jujubosides in wild jujube. Furthermore, the characterization of β-glucosidase genes is expected to facilitate investigations involving the cultivation and breeding of wild jujube.https://www.mdpi.com/2073-4425/14/6/1135wild jujubejujubosideβ-glucosidaseprokaryotic expressionmolecular docking |
| spellingShingle | Mingjun Yang Yimian Ma Xupeng Si Xiaofeng Liu Xin Geng Xin Wen Guoqiong Li Liping Zhang Chengmin Yang Zheng Zhang Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A Genes wild jujube jujuboside β-glucosidase prokaryotic expression molecular docking |
| title | Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A |
| title_full | Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A |
| title_fullStr | Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A |
| title_full_unstemmed | Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A |
| title_short | Analysis of the Glycoside Hydrolase Family 1 from Wild Jujube Reveals Genes Involved in the Degradation of Jujuboside A |
| title_sort | analysis of the glycoside hydrolase family 1 from wild jujube reveals genes involved in the degradation of jujuboside a |
| topic | wild jujube jujuboside β-glucosidase prokaryotic expression molecular docking |
| url | https://www.mdpi.com/2073-4425/14/6/1135 |
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