Brassinosteroid Biosynthetic Gene <i>SlCYP90B3</i> Alleviates Chilling Injury of Tomato (<i>Solanum lycopersicum</i>) Fruits during Cold Storage
Tomato is susceptible to chilling injury during cold storage. In this study, we found that low temperature promoted the expression of brassinosteroid (BR) biosynthetic genes in tomato fruits. The overexpression of <i>SlCYP90B3</i> (<i>SlCYP90B3-OE</i>), a key BR biosynthetic...
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MDPI AG
2022-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/1/115 |
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| author | Songshen Hu Tonglin Wang Zhiyong Shao Fanliang Meng Hao Chen Qiaomei Wang Jirong Zheng Lihong Liu |
| author_facet | Songshen Hu Tonglin Wang Zhiyong Shao Fanliang Meng Hao Chen Qiaomei Wang Jirong Zheng Lihong Liu |
| author_sort | Songshen Hu |
| collection | DOAJ |
| description | Tomato is susceptible to chilling injury during cold storage. In this study, we found that low temperature promoted the expression of brassinosteroid (BR) biosynthetic genes in tomato fruits. The overexpression of <i>SlCYP90B3</i> (<i>SlCYP90B3-OE</i>), a key BR biosynthetic gene, alleviated the chilling injury with decreased electrical conductivity and malondialdehyde. In <i>SlCYP90B3-OE</i> tomato fruits, the activities of antioxidant enzymes, including ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), were markedly increased, while the activity of membranous lipolytic enzymes, lipoxygenase (LOX), and phospholipase D (PLD), were significantly decreased when compared with the wild-type in response to cold storage. Furthermore, the expression level of the cold-response-system component, <i>SlCBF1</i>, was higher in <i>SlCYP90B3-OE</i> fruits than in the wild-type fruits. These results indicated that <i>SlCYP90B3</i> might be involved in the chilling tolerance of tomato fruits during cold storage, possibly by regulating the antioxidant enzyme system and <i>SlCBF1</i> expression. |
| first_indexed | 2024-03-10T03:00:53Z |
| format | Article |
| id | doaj.art-800e5da4b8df4c3982293094ce46a8cb |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T03:00:53Z |
| publishDate | 2022-01-01 |
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| series | Antioxidants |
| spelling | doaj.art-800e5da4b8df4c3982293094ce46a8cb2023-11-23T12:47:33ZengMDPI AGAntioxidants2076-39212022-01-0111111510.3390/antiox11010115Brassinosteroid Biosynthetic Gene <i>SlCYP90B3</i> Alleviates Chilling Injury of Tomato (<i>Solanum lycopersicum</i>) Fruits during Cold StorageSongshen Hu0Tonglin Wang1Zhiyong Shao2Fanliang Meng3Hao Chen4Qiaomei Wang5Jirong Zheng6Lihong Liu7Department of Horticulture, College of Agriculture and Biotechnology, Hangzhou Academy of Agricultural Sciences, Zhejiang University, Hangzhou 310024, ChinaDepartment of Horticulture, College of Agriculture and Biotechnology, Hangzhou Academy of Agricultural Sciences, Zhejiang University, Hangzhou 310024, ChinaKey Laboratory of Horticultural Plant Growth and Development, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310058, ChinaKey Laboratory of Horticultural Plant Growth and Development, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310058, ChinaKey Laboratory of Horticultural Plant Growth and Development, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310058, ChinaKey Laboratory of Horticultural Plant Growth and Development, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310058, ChinaDepartment of Horticulture, College of Agriculture and Biotechnology, Hangzhou Academy of Agricultural Sciences, Zhejiang University, Hangzhou 310024, ChinaKey Laboratory of Horticultural Plant Growth and Development, Ministry of Agriculture, Department of Horticulture, Zhejiang University, Hangzhou 310058, ChinaTomato is susceptible to chilling injury during cold storage. In this study, we found that low temperature promoted the expression of brassinosteroid (BR) biosynthetic genes in tomato fruits. The overexpression of <i>SlCYP90B3</i> (<i>SlCYP90B3-OE</i>), a key BR biosynthetic gene, alleviated the chilling injury with decreased electrical conductivity and malondialdehyde. In <i>SlCYP90B3-OE</i> tomato fruits, the activities of antioxidant enzymes, including ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD), were markedly increased, while the activity of membranous lipolytic enzymes, lipoxygenase (LOX), and phospholipase D (PLD), were significantly decreased when compared with the wild-type in response to cold storage. Furthermore, the expression level of the cold-response-system component, <i>SlCBF1</i>, was higher in <i>SlCYP90B3-OE</i> fruits than in the wild-type fruits. These results indicated that <i>SlCYP90B3</i> might be involved in the chilling tolerance of tomato fruits during cold storage, possibly by regulating the antioxidant enzyme system and <i>SlCBF1</i> expression.https://www.mdpi.com/2076-3921/11/1/115tomato fruitcold storage<i>SlCYP90B3</i>antioxidant enzymesmembranous lipolytic enzymes<i>SlCBF1</i> |
| spellingShingle | Songshen Hu Tonglin Wang Zhiyong Shao Fanliang Meng Hao Chen Qiaomei Wang Jirong Zheng Lihong Liu Brassinosteroid Biosynthetic Gene <i>SlCYP90B3</i> Alleviates Chilling Injury of Tomato (<i>Solanum lycopersicum</i>) Fruits during Cold Storage Antioxidants tomato fruit cold storage <i>SlCYP90B3</i> antioxidant enzymes membranous lipolytic enzymes <i>SlCBF1</i> |
| title | Brassinosteroid Biosynthetic Gene <i>SlCYP90B3</i> Alleviates Chilling Injury of Tomato (<i>Solanum lycopersicum</i>) Fruits during Cold Storage |
| title_full | Brassinosteroid Biosynthetic Gene <i>SlCYP90B3</i> Alleviates Chilling Injury of Tomato (<i>Solanum lycopersicum</i>) Fruits during Cold Storage |
| title_fullStr | Brassinosteroid Biosynthetic Gene <i>SlCYP90B3</i> Alleviates Chilling Injury of Tomato (<i>Solanum lycopersicum</i>) Fruits during Cold Storage |
| title_full_unstemmed | Brassinosteroid Biosynthetic Gene <i>SlCYP90B3</i> Alleviates Chilling Injury of Tomato (<i>Solanum lycopersicum</i>) Fruits during Cold Storage |
| title_short | Brassinosteroid Biosynthetic Gene <i>SlCYP90B3</i> Alleviates Chilling Injury of Tomato (<i>Solanum lycopersicum</i>) Fruits during Cold Storage |
| title_sort | brassinosteroid biosynthetic gene i slcyp90b3 i alleviates chilling injury of tomato i solanum lycopersicum i fruits during cold storage |
| topic | tomato fruit cold storage <i>SlCYP90B3</i> antioxidant enzymes membranous lipolytic enzymes <i>SlCBF1</i> |
| url | https://www.mdpi.com/2076-3921/11/1/115 |
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