The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensis
Abstract Background Angiostrongylus cantonensis is a human zoonotic nematode parasite. Our previous studies found that PAS-5 and Galectin-1 (Gal-1) proteins of A. cantonensis could be strongly recognized by sera from mice infected with A. cantonensis. In this study, we further evaluated the potentia...
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| Format: | Article |
| Language: | English |
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BMC
2018-05-01
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| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13071-018-2894-5 |
| _version_ | 1818966584139448320 |
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| author | Lan-Zhu Yan Xiao-Meng Shi Yan-Wen Zu Yuan-Yuan Shen Xi-Xi Chen Meng-Jing Zhao Xing-Pan Li Bao-Long Yan Hui-Cong Huang |
| author_facet | Lan-Zhu Yan Xiao-Meng Shi Yan-Wen Zu Yuan-Yuan Shen Xi-Xi Chen Meng-Jing Zhao Xing-Pan Li Bao-Long Yan Hui-Cong Huang |
| author_sort | Lan-Zhu Yan |
| collection | DOAJ |
| description | Abstract Background Angiostrongylus cantonensis is a human zoonotic nematode parasite. Our previous studies found that PAS-5 and Galectin-1 (Gal-1) proteins of A. cantonensis could be strongly recognized by sera from mice infected with A. cantonensis. In this study, we further evaluated the potential roles of these two proteins in the induction of immune response in mice. Methods Mice were immunized with recombinant PAS-5 or Gal-1 and then challenged with 30 infective A. cantonensis larvae following the last immunization. We then examined the infected mice for changes in serum antibodies and cytokines by ELISA, CD4+ T cells and CD4+CD25+FoxP3+ regulatory T cells (Tregs) by flow cytometry, and tissue damage severity by hematoxylin-eosin (H&E) staining. Results Compared with control mice, the PAS-5-immunized mice exhibited increased levels of serum antibodies and cytokines (except for IL-10) at different time points post-infection. PAS-5 immunization promoted significant proliferation of CD4+ T cells, and caused more damage in the brain tissue. Vaccination with Gal-1 inhibited the production of antibodies (except for IgG1) and IFN-γ, but promoted the expression of IL-4 and IL-10. Gal-1 immunization results in significant increases in the levels of CD4+CD25+FoxP3+ Tregs, and mild inflammatory changes. Conclusions Taken together, our findings show that PAS-5 enhances, but Gal-1 inhibits the immune response in the early stage of A. cantonensis infections. |
| first_indexed | 2024-12-20T13:35:13Z |
| format | Article |
| id | doaj.art-800efef31a8a4e20a919c3bd45eceb32 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-20T13:35:13Z |
| publishDate | 2018-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-800efef31a8a4e20a919c3bd45eceb322022-12-21T19:38:59ZengBMCParasites & Vectors1756-33052018-05-011111810.1186/s13071-018-2894-5The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensisLan-Zhu Yan0Xiao-Meng Shi1Yan-Wen Zu2Yuan-Yuan Shen3Xi-Xi Chen4Meng-Jing Zhao5Xing-Pan Li6Bao-Long Yan7Hui-Cong Huang8Department of Parasitology, School of Basic Medical Sciences, Wenzhou Medical UniversityDepartment of Parasitology, School of Basic Medical Sciences, Wenzhou Medical UniversityDepartment of Parasitology, School of Basic Medical Sciences, Wenzhou Medical UniversitySchool of the Second Clinical Medical Sciences, Wenzhou Medical UniversitySchool of the First Clinical Medical Sciences, Wenzhou Medical UniversitySchool of the First Clinical Medical Sciences, Wenzhou Medical UniversityDepartment of Parasitology, School of Basic Medical Sciences, Wenzhou Medical UniversityDepartment of Parasitology, School of Basic Medical Sciences, Wenzhou Medical UniversityDepartment of Parasitology, School of Basic Medical Sciences, Wenzhou Medical UniversityAbstract Background Angiostrongylus cantonensis is a human zoonotic nematode parasite. Our previous studies found that PAS-5 and Galectin-1 (Gal-1) proteins of A. cantonensis could be strongly recognized by sera from mice infected with A. cantonensis. In this study, we further evaluated the potential roles of these two proteins in the induction of immune response in mice. Methods Mice were immunized with recombinant PAS-5 or Gal-1 and then challenged with 30 infective A. cantonensis larvae following the last immunization. We then examined the infected mice for changes in serum antibodies and cytokines by ELISA, CD4+ T cells and CD4+CD25+FoxP3+ regulatory T cells (Tregs) by flow cytometry, and tissue damage severity by hematoxylin-eosin (H&E) staining. Results Compared with control mice, the PAS-5-immunized mice exhibited increased levels of serum antibodies and cytokines (except for IL-10) at different time points post-infection. PAS-5 immunization promoted significant proliferation of CD4+ T cells, and caused more damage in the brain tissue. Vaccination with Gal-1 inhibited the production of antibodies (except for IgG1) and IFN-γ, but promoted the expression of IL-4 and IL-10. Gal-1 immunization results in significant increases in the levels of CD4+CD25+FoxP3+ Tregs, and mild inflammatory changes. Conclusions Taken together, our findings show that PAS-5 enhances, but Gal-1 inhibits the immune response in the early stage of A. cantonensis infections.http://link.springer.com/article/10.1186/s13071-018-2894-5Angiostrongylus cantonensisEarly infectionPAS-5Galectin-1Immune response |
| spellingShingle | Lan-Zhu Yan Xiao-Meng Shi Yan-Wen Zu Yuan-Yuan Shen Xi-Xi Chen Meng-Jing Zhao Xing-Pan Li Bao-Long Yan Hui-Cong Huang The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensis Parasites & Vectors Angiostrongylus cantonensis Early infection PAS-5 Galectin-1 Immune response |
| title | The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensis |
| title_full | The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensis |
| title_fullStr | The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensis |
| title_full_unstemmed | The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensis |
| title_short | The opposite roles of PAS-5 and Galectin-1 in immune response during the early infection of Angiostrongylus cantonensis |
| title_sort | opposite roles of pas 5 and galectin 1 in immune response during the early infection of angiostrongylus cantonensis |
| topic | Angiostrongylus cantonensis Early infection PAS-5 Galectin-1 Immune response |
| url | http://link.springer.com/article/10.1186/s13071-018-2894-5 |
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